Accession	MF_01646
Dates	26-MAR-2008 (Created) 7-JUN-2008 (Last updated, Version 3)

Data class

Protein

Predictors

HAMAP; MF_01646; [distribution of match scores in UniProtKB];[seed alignment for MF_01646]

Identifier

CYSG

Protein name

RecName: Full=Siroheme synthase; RecName: Full=Uroporphyrinogen-III C-methyltransferase; Short=Urogen III methylase; EC=2.1.1.107; AltName: Full=SUMT; AltName: Full=Uroporphyrinogen III methylase; Short=UROM; RecName: Full=Precorrin-2 dehydrogenase; EC=1.3.1.76; RecName: Full=Sirohydrochlorin ferrochelatase; EC=4.99.1.4;

Gene name

cysG

FUNCTION: Multifunctional enzyme that catalyze the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consist of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme (By similarity).

CATALYTIC ACTIVITY: S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.

CATALYTIC ACTIVITY: S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

CATALYTIC ACTIVITY: Precorrin-2 + NAD(+) = sirohydrochlorin + NADH.

CATALYTIC ACTIVITY: Siroheme + 2 H(+) = sirohydrochlorin + Fe(2+).

PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.

PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

PATHWAY: Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.

PATHWAY: Porphyrin metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.

PATHWAY: Porphyrin metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

SIMILARITY: Belongs to the precorrin methyltransferase family.

PROSITE	PS00839; SUMT_1; 1; PS00840; SUMT_2; 1;
Pfam	PF00590; TP_methylase; 1;
TIGRFAMs	TIGR01469; cobA_cysG_Cterm; 1; TIGR01470; cysG_Nterm; 1;

Cobalamin biosynthesis, Lyase, Methyltransferase, Multifunctional enzyme, NAD, Oxidoreductase, Porphyrin biosynthesis, S-adenosyl-L-methionine, Transferase.

GO:0004325; Molecular function: ferrochelatase activity.
GO:0004851; Molecular function: uroporphyrin-III C-methyltransferase activity.
GO:0043115; Molecular function: precorrin-2 dehydrogenase activity.
GO:0006779; Biological process: porphyrin biosynthetic process.
GO:0009236; Biological process: cobalamin biosynthetic process.
GO:0019354; Biological process: siroheme biosynthetic process.

From: CYSG_ECOLI (P0AEA8)
Key		From		To		Description		Condition		FTGroup
REGION		218		457		Uroporphyrinogen-III C-methyltransferase

Size range:	457-549 amino acids
Related UniRules:	None
Template:	P0AEA8 (CYSG_ECOLI)
Scope:	Bacteria; Proteobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in AERHH, AERS4, ENTS8, ERWCT, HALHL, KLEP7, SERP5, YERE8, YERP3, YERPN, YERPP, YERPS
Plasmid encoded:	None