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UniProtKB/Swiss-Prot entry Q9ZNZ7


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GLTB1_ARATH
Primary accession number Q9ZNZ7
Secondary accession number P93649
Integrated into Swiss-Prot on January 11, 2001
Sequence was last modified on January 11, 2001 (Sequence version 2)
Annotations were last modified on    December 16, 2008 (Entry version 88)
Name and origin of the protein
Protein name Ferredoxin-dependent glutamate synthase 1, chloroplastic [Precursor]
Synonyms EC 1.4.7.1
Fd-GOGAT 1
Gene name
Name: GLU1
Synonyms: GLS1
OrderedLocusNames: At5g04140
ORFNames: F21E1_60
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
STRAIN=cv. Columbia;
PubMed=9057836 [NCBI, ExPASy, EBI, Israel, Japan]
Suzuki A., Rothstein S.;
"Structure and regulation of ferredoxin-dependent glutamase synthase from Arabidopsis thaliana. Cloning of cDNA expression in different tissues of wild-type and gltS mutant strains, and light induction.";
Eur. J. Biochem. 243:708-718(1997).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
STRAIN=cv. Columbia;
DOI=10.1105/tpc.10.5.741; PubMed=9596633 [NCBI, ExPASy, EBI, Israel, Japan]
Coschigano K.T., Melo-Oliveira R., Lim J., Coruzzi G.M.;
"Arabidopsis gls mutants and distinct Fd-GOGAT genes. Implications for photorespiration and primary nitrogen assimilation.";
Plant Cell 10:741-752(1998).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048507; PubMed=11130714 [NCBI, ExPASy, EBI, Israel, Japan]
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
Nature 408:823-826(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Y09667; CAA70862.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U39287; AAC78551.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL391716; CAC05496.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_568134.1; -.
NP_850763.1; -.
UniGene At.21961
3D structure databases
HSSP P55038; 1OFD. [HSSP ENTRY / PDB]
ModBase Q9ZNZ7.
Organism-specific databases
TAIR At5g04140; -.
Gene expression databases
ArrayExpress Q9ZNZ7; -.
GermOnline AT5G04140; Arabidopsis thaliana.
Ontologies
GO
GO:0048046; Cellular component: apoplast (inferred from direct assay from TAIR).
GO:0009941; Cellular component: chloroplast envelope (inferred from direct assay from TAIR).
GO:0009570; Cellular component: chloroplast stroma (inferred from direct assay from TAIR).
GO:0016020; Cellular component: membrane (inferred from direct assay from TAIR).
GO:0051538; Molecular function: 3 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0016041; Molecular function: glutamate synthase (ferredoxin) activity (inferred from electronic annotation from EC).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006537; Biological process: glutamate biosynthetic process (inferred from electronic annotation from InterPro).
GO:0006541; Biological process: glutamine metabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0009853; Biological process: photorespiration (traceable author statement from TAIR).
GO:0009416; Biological process: response to light stimulus (inferred from expression pattern from TAIR).
QuickGo view.
Family and domain databases
InterPro IPR013785; Aldolase_TIM.
IPR000583; GATase_2.
IPR002932; Glu_synth_centr_C.
IPR006982; Glu_synth_centr_N.
IPR002489; Glu_synthase_C.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.70; Aldolase_TIM; 2.
G3DSA:2.160.20.60; Glu_synthase_C; 1.
Pfam PF00310; GATase_2; 1.
PF04898; Glu_syn_central; 1.
PF01645; Glu_synthase; 1.
PF01493; GXGXG; 1.
Pfam graphical view of domain structure.
PROSITE PS51278; GATASE_TYPE_2; 1.
PROSITE graphical view of domain structure (profiles).
Proteomics databases
PRIDE Q9ZNZ7; -.
Proteomic databases
ProMEX Q9ZNZ7; -.
Genome annotation databases
GeneID 830292; -.
GenomeReviews BA000015_GR; AT5G04140.
KEGG ath:AT5G04140; -.
NMPDR fig|3702.1.peg.22509; -.
Other
ProtoNet Q9ZNZ7.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3Fe-4S; Alternative splicing; Amino-acid biosynthesis; Chloroplast; Complete proteome; FAD; Flavoprotein; FMN; Glutamate biosynthesis; Glutamine amidotransferase; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Plastid; Transit peptide.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
TRANSIT   1    131  131     Chloroplast (By similarity). 
CHAIN   132   1648  1517     Ferredoxin-dependent glutamate synthase 1, chloroplastic. PRO_0000011614
DOMAIN   132    531  400     Glutamine amidotransferase type-2. 
NP_BIND   1210   1267  58     FMN (By similarity). 
ACT_SITE   132    132        For GATase activity (By similarity). 
METAL   1263   1263        Iron-sulfur (3Fe-4S) (By similarity). 
METAL   1269   1269        Iron-sulfur (3Fe-4S) (By similarity). 
METAL   1274   1274        Iron-sulfur (3Fe-4S) (By similarity). 
VAR_SEQ   94    119        Missing (in isoform Short). VSP_001764
CONFLICT   122    122        L -> W (in Ref. 1; AAC78551). 
CONFLICT   237    237        G -> R (in Ref. 1; AAC78551). 
CONFLICT   411    411        S -> C (in Ref. 2; CAA70862). 
CONFLICT   647    650        EGLV -> KVWF (in Ref. 2; CAA70862). 
CONFLICT   760    760        A -> T (in Ref. 2; CAA70862). 
CONFLICT   1499   1500        IF -> DI (in Ref. 2; CAA70862). 
CONFLICT   1611   1611        E -> K (in Ref. 1; AAC78551). 
Sequence information
Length: 1648 AA [This is the length of the unprocessed precursor] Molecular weight: 179920 Da [This is the MW of the unprocessed precursor] CRC64: 58790CE711DCBF6A [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAMQSLSPVP KLLSTTPSSV LSSDKNFFFV DFVGLYCKSK RTRRRLRGDS SSSSRSSSSL 

        70         80         90        100        110        120 
SRLSSVRAVI DLERVHGVSE KDLSSPSALR PQVRFFTDIN FTNTQRAKFH PLWGSFKQVA 

       130        140        150        160        170        180 
NLEDILSERG ACGVGFIANL DNIPSHGVVK DALIALGCME HRGGCGADND SGDGSGLMSS 

       190        200        210        220        230        240 
IPWDFFNVWA KEQSLAPFDK LHTGVGMIFL PQDDTFMQEA KQVIENIFEK EGLQVLGWRE 

       250        260        270        280        290        300 
VPVNVPIVGK NARETMPNIQ QVFVKIAKED STDDIERELY ICRKLIERAV ATESWGTELY 

       310        320        330        340        350        360 
FCSLSNQTIV YKGMLRSEAL GLFYLDLQNE LYESPFAIYH RRYSTNTSPR WPLAQPMRFL 

       370        380        390        400        410        420 
GHNGEINTIQ GNLNWMQSRE ASLKAAVWNG RENEIRPFGN PRGSDSANLD SAAEIMIRSG 

       430        440        450        460        470        480 
RTPEEALMIL VPEAYKNHPT LSVKYPEVVD FYDYYKGQME AWDGPALLLF SDGKTVGACL 

       490        500        510        520        530        540 
DRNGLRPARY WRTSDNFVYV ASEVGVVPVD EAKVTMKGRL GPGMMIAVDL VNGQVYENTE 

       550        560        570        580        590        600 
VKKRISSFNP YGKWIKENSR FLKPVNFKSS TVMENEEILR SQQAFGYSSE DVQMVIESMA 

       610        620        630        640        650        660 
SQGKEPTFCM GDDIPLAGLS QRPHMLYDYF KQRFAQVTNP AIDPLREGLV MSLEVNIGKR 

       670        680        690        700        710        720 
GNILELGPEN ASQVILSNPV LNEGALEELM KDQYLKPKVL STYFDIRKGV EGSLQKALYY 

       730        740        750        760        770        780 
LCEAADDAVR SGSQLLVLSD RSDRLEPTRP SIPIMLAVGA VHQHLIQNGL RMSASIVADT 

       790        800        810        820        830        840 
AQCFSTHHFA CLVGYGASAV CPYLALETCR QWRLSNKTVA FMRNGKIPTV TIEQAQKNYT 

       850        860        870        880        890        900 
KAVNAGLLKI LSKMGISLLS SYCGAQIFEI YGLGQDVVDL AFTGSVSKIS GLTFDELARE 

       910        920        930        940        950        960 
TLSFWVKAFS EDTTKRLENF GFIQFRPGGE YHSNNPEMSK LLHKAVREKS ETAYAVYQQH 

       970        980        990       1000       1010       1020 
LSNRPVNVLR DLLEFKSDRA PIPVGKVEPA VAIVQRFCTG GMSLGAISRE THEAIAIAMN 

      1030       1040       1050       1060       1070       1080 
RIGGKSNSGE GGEDPIRWKP LTDVVDGYSP TLPHLKGLQN GDIATSAIKQ VASGRFGVTP 

      1090       1100       1110       1120       1130       1140 
TFLVNADQLE IKVAQGAKPG EGGQLPGKKV SAYIARLRSS KPGVPLISPP PHHDIYSIED 

      1150       1160       1170       1180       1190       1200 
LAQLIFDLHQ INPNAKVSVK LVAEAGIGTV ASGVAKGNAD IIQISGHDGG TGASPISSIK 

      1210       1220       1230       1240       1250       1260 
HAGGPWELGL TETHQTLIAN GLRERVILRV DGGLKSGVDV LMAAAMGADE YGFGSLAMIA 

      1270       1280       1290       1300       1310       1320 
TGCVMARICH TNNCPVGVAS QREELRARFP GVPGDLVNYF LYVAEEVRGI LAQLGYNSLD 

      1330       1340       1350       1360       1370       1380 
DIIGRTELLR PRDISLVKTQ HLDLSYLLSS VGTPSLSSTE IRKQEVHTNG PVLDDDILAD 

      1390       1400       1410       1420       1430       1440 
PLVIDAIENE KVVEKTVKIC NVDRAACGRV AGVIAKKYGD TGFAGQVNLT FLGSAGQSFG 

      1450       1460       1470       1480       1490       1500 
CFLIPGMNIR LIGESNDYVG KGMAGGEIVV TPVEKIGFVP EEATIVGNTC LYGATGGQIF 

      1510       1520       1530       1540       1550       1560 
ARGKAGERFA VRNSLAEAVV EGTGDHCCEY MTGGCVVVLG KVGRNVAAGM TGGLAYLLDE 

      1570       1580       1590       1600       1610       1620 
DDTLLPKINR EIVKIQRVTA PAGELQLKSL IEAHVEKTGS SKGATILNEW EKYLPLFWQL 

      1630       1640 
VPPSEEDTPE ASAAYVRTST GEVTFQSA 

Q9ZNZ7 in FASTA format

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