NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L-CABP1 AND S-CABP1), AND MYRISTOYLATION AT GLY-2.
TISSUE=Retina;
DOI=10.1074/jbc.275.2.1247; PubMed=10625670 [NCBI, ExPASy, EBI, Israel, Japan]
Haeseleer F., Sokal I., Verlinde C.L.M.J., Erdjument-Bromage H., Tempst P., Pronin A.N., Benovic J.L., Fariss R.N., Palczewski K.;
"Five members of a novel Ca(2+)-binding protein (CABP) subfamily with similarity to calmodulin.";
J. Biol. Chem. 275:1247-1260(2000).

[2]

NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CALBRAIN).
TISSUE=Cerebellum;
DOI=10.1074/jbc.274.6.3610; PubMed=9920909 [NCBI, ExPASy, EBI, Israel, Japan]
Yamaguchi K., Yamaguchi F., Miyamoto O., Sugimoto K., Konishi R., Hatase O.;
"Calbrain, a novel two EF-hand calcium-binding protein that suppresses Ca2+/calmodulin-dependent protein kinase II activity in the brain.";
J. Biol. Chem. 274:3610-3616(1999).

Comments

INTERACTION:
Q13936:CACNA1C; NbExp=2; IntAct=EBI-907894, EBI-1038838;
P06623:CNP (xeno); NbExp=1; IntAct=EBI-907894, EBI-907934;
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=L-CaBP1 is associated most likely with the cytoskeletal structures, whereas S-CaBP1 is localized at or near the plasma membrane.

ALTERNATIVE PRODUCTS: 3 named isoforms [FASTA] produced by alternative splicing. Experimental confirmation may be lacking for some isoforms.

Name	L-CaBP1
Isoform ID	Q9NZU7-1
This is the isoform sequence displayed in this entry.

Name	S-CaBP1
Isoform ID	Q9NZU7-2
Features which should be applied to build the isoform sequence: VSP_000731.

Name	Calbrain
Isoform ID	Q9NZU7-3
Note: It is currently uncertain whether calbrain represent a spliced isoform.
Features which should be applied to build the isoform sequence: VSP_000732.

TISSUE SPECIFICITY: Retina and brain. Calbrain was found exclusively in brain where it is abundant in the hippocampus, habenular area in the epithalamus and in the cerebellum.
SIMILARITY: Contains 4 EF-hand domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF169149; AAF25783.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF169148; AAF25782.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X94700; CAA64361.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_112482.1; -.

UniGene

Hs.458482

3D structure databases

HSSP

P02588; 1PON. [HSSP ENTRY / PDB]

ModBase

Q9NZU7.

Protein-protein interaction databases

IntAct

Q9NZU7; -.

Organism-specific databases

HIX0011074; -.

HGNC:1384; CABP1.

605563; gene. [NCBI / EBI]

PharmGKB

PA26000; -.

GeneCards

Q9NZU7.

Gene expression databases

ArrayExpress

Q9NZU7; -.

CleanEx

HS_CABP1; -.

GermOnline

ENSG00000157782; Homo sapiens.

Ontologies

GO:0014069;	Cellular component: postsynaptic density (inferred from direct assay from UniProtKB).
GO:0005509;	Molecular function: calcium ion binding (traceable author statement from ProtInc).
GO:0048306;	Molecular function: calcium-dependent protein binding (inferred from physical interaction from UniProtKB).
GO:0004857;	Molecular function: enzyme inhibitor activity (traceable author statement from ProtInc).
QuickGo view.

Family and domain databases

InterPro

IPR015754; Ca_binding_pro.
IPR011992; EF-Hand_type.
IPR002048; EF_hand_Ca_bd.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.238.10; EF-Hand_type; 1.

PANTHER

PTHR23050:SF21; Ca_binding_pro; 1.

Pfam

PF00036; efhand; 3.
Pfam graphical view of domain structure.

ProDom

PD000012; EF-hand; 2.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00054; EFh; 3.
SMART graphical view of domain structure.

PROSITE

PS00018; EF_HAND_1; 3.
PS50222; EF_HAND_2; 4.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q9NZU7.

Genome annotation databases

Ensembl

ENSG00000157782; Homo sapiens. [Contig view]

GeneID

9478; -.

Phylogenomic databases

HOVERGEN

Q9NZU7; -.

Other

CABP1; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Calcium; Cell membrane; Cytoplasm; Cytoskeleton; Lipoprotein; Membrane; Myristate; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 227`	`226`	`Calcium-binding protein 1.`	`PRO_0000073513`
`DOMAIN`	`82 117`	`36`	`EF-hand 1.`
`DOMAIN`	`118 153`	`36`	`EF-hand 2.`
`DOMAIN`	`159 194`	`36`	`EF-hand 3.`
`DOMAIN`	`196 227`	`32`	`EF-hand 4.`
`CA_BIND`	`95 106`	`12`	`1 (Potential).`
`CA_BIND`	`172 183`	`12`	`2 (Potential).`
`CA_BIND`	`209 220`	`12`	`3 (Potential).`
`LIPID`	`2 2`		`N-myristoyl glycine.`
`VAR_SEQ`	`1 157`		`Missing (in isoform Calbrain).`	`VSP_000732`
`VAR_SEQ`	`16 75`		`Missing (in isoform S-CaBP1).`	`VSP_000731`

Sequence information

Length: 227 AA [This is the length of the unprocessed precursor]

Molecular weight: 25979 Da [This is the MW of the unprocessed precursor]

CRC64: FF3F7A9B575486AB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGNCVKYPLR NLSRKMCQEE QTSYMVVQTS EEGLAADAEL PGPLLMLAQN CAVMHNLLGP 

        70         80         90        100        110        120 
ACIFLRKGFA ENRQPDRSLR PEEIEELREA FREFDKDKDG YINCRDLGNC MRTMGYMPTE 

       130        140        150        160        170        180 
MELIELSQQI NMNLGGHVDF DDFVELMGPK LLAETADMIG VKELRDAFRE FDTNGDGEIS 

       190        200        210        220 
TSELREAMRK LLGHQVGHRD IEEIIRDVDL NGDGRVDFEE FVRMMSR

Q9NZU7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools