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UniProtKB/Swiss-Prot entry Q9M591


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CRD1_ARATH
Primary accession number Q9M591
Secondary accession numbers O04051 Q38892 Q8GUS3 Q9M1K4
Integrated into Swiss-Prot on August 16, 2005
Sequence was last modified on August 16, 2005 (Sequence version 2)
Annotations were last modified on    December 16, 2008 (Entry version 51)
Name and origin of the protein
Protein name Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic [Precursor]
Synonyms Mg-protoporphyrin IX monomethyl ester oxidative cyclase
EC 1.14.13.81
Copper response defect 1 protein
Dicarboxylate diiron protein
AtZIP
Gene name
Name: CRD1
Synonyms: AT103, CHL27, ZIP
OrderedLocusNames: At3g56940
ORFNames: F24I3.20
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1104/pp.116.1.27; PubMed=9449833 [NCBI, ExPASy, EBI, Israel, Japan]
Zheng C.C., Porat R., Lu P., O'Neill S.D.;
"PNZIP is a novel mesophyll-specific cDNA that is regulated by phytochrome and the circadian rhythm and encodes a protein with a leucine zipper motif.";
Plant Physiol. 116:27-35(1998).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1093/emboj/19.10.2139; PubMed=10811605 [NCBI, ExPASy, EBI, Israel, Japan]
Moseley J.L., Quinn J., Eriksson M., Merchant S.;
"The Crd1 gene encodes a putative di-iron enzyme required for photosystem I accumulation in copper deficiency and hypoxia in Chlamydomonas reinhardtii.";
EMBO J. 19:2139-2151(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048706; PubMed=11130713 [NCBI, ExPASy, EBI, Israel, Japan]
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
Nature 408:820-822(2000).
[4]
NUCLEOTIDE SEQUENCE [MRNA] OF 1-76.
Liu N., Zheng C.;
"Cloning and characteristics of AT103 gene promoter.";
Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [MRNA] OF 215-313.
STRAIN=cv. Columbia;
PubMed=9339905 [NCBI, ExPASy, EBI, Israel, Japan]
Pih K.T., Jang H.J., Kang S.G., Piao H.L., Hwang I.;
"Isolation of molecular markers for salt stress responses in Arabidopsis thaliana.";
Mol. Cells 7:567-571(1997).
[6]
FUNCTION, ENZYME ACTIVITY, AND SUBCELLULAR LOCATION.
DOI=10.1073/pnas.2136793100; PubMed=14673103 [NCBI, ExPASy, EBI, Israel, Japan]
Tottey S., Block M.A., Allen M., Westergren T., Albrieux C., Scheller H.V., Merchant S., Jensen P.E.;
"Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide.";
Proc. Natl. Acad. Sci. U.S.A. 100:16119-16124(2003).
Comments
  • FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME).
  • CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O.
  • CATALYTIC ACTIVITY: 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O.
  • CATALYTIC ACTIVITY: 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O.
  • COFACTOR: Iron (By similarity).
  • PATHWAY: Porphyrin biosynthesis; chlorophyll biosynthesis.
  • SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane; Peripheral membrane protein. Plastid, chloroplast thylakoid membrane; Peripheral membrane protein.
  • SIMILARITY: Belongs to the acsF family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U38232; AAB18942.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF236101; AAF63476.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL138655; CAB72164.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY170319; AAO11785.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U75599; AAB51703.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T47754; T47754.
RefSeq NP_191253.1; -.
UniGene At.48778
3D structure databases
ModBase Q9M591.
Enzyme and pathway databases
BioCyc MetaCyc:AT3G56940-MON; -.
Organism-specific databases
TAIR At3g56940; -.
Gene expression databases
GermOnline AT3G56940; Arabidopsis thaliana.
Ontologies
GO
GO:0009706; Cellular component: chloroplast inner membrane (inferred from direct assay from TAIR).
GO:0009535; Cellular component: chloroplast thylakoid membrane (inferred from direct assay from TAIR).
GO:0003677; Molecular function: DNA binding (traceable author statement from TAIR).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0048529; Molecular function: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity (inferred from electronic annotation from EC).
GO:0015995; Biological process: chlorophyll biosynthetic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0015979; Biological process: photosynthesis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.
Pfam PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR02029; AcsF; 1.
Proteomics databases
PRIDE Q9M591; -.
Genome annotation databases
GeneID 824861; -.
GenomeReviews BA000014_GR; AT3G56940.
KEGG ath:AT3G56940; -.
NMPDR fig|3702.1.peg.17023; -.
Other
ProtoNet Q9M591.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Chlorophyll biosynthesis; Chloroplast; Complete proteome; Iron; Membrane; Metal-binding; NADP; Oxidoreductase; Photosynthesis; Plastid; Plastid inner membrane; Thylakoid; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    36  36     Chloroplast (Potential). 
CHAIN   37   409  373     Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic. PRO_0000000598
CONFLICT   14    14        F -> I (in Ref. 1). 
CONFLICT   157   157        L -> S (in Ref. 1; AAB18942). 
CONFLICT   216   219        TYLS -> RAAR (in Ref. 5). 
CONFLICT   299   299        C -> W (in Ref. 2; AAF63476). 
CONFLICT   310   312        LNT -> FKH (in Ref. 5). 
Sequence information
Length: 409 AA [This is the length of the unprocessed precursor] Molecular weight: 47631 Da [This is the MW of the unprocessed precursor] CRC64: B8808079C07B8C68 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAAEMALVKP ISKFSSPKLS NPSKFLSGRR FSTVIRMSAS SSPPPPTTAT SKSKKGTKKE 

        70         80         90        100        110        120 
IQESLLTPRF YTTDFEEMEQ LFNTEINKNL NEAEFEALLQ EFKTDYNQTH FVRNKEFKEA 

       130        140        150        160        170        180 
ADKLQGPLRQ IFVEFLERSC TAEFSGFLLY KELGRRLKKT NPVVAEIFSL MSRDEARHAG 

       190        200        210        220        230        240 
FLNKGLSDFN LALDLGFLTK ARKYTFFKPK FIFYATYLSE KIGYWRYITI YRHLKENPEF 

       250        260        270        280        290        300 
QCYPIFKYFE NWCQDENRHG DFFSALMKAQ PQFLNDWQAK LWSRFFCLSV YVTMYLNDCQ 

       310        320        330        340        350        360 
RTNFYEGIGL NTKEFDMHVI IETNRTTARI FPAVLDVENP EFKRKLDRMV VSYEKLLAIG 

       370        380        390        400 
ETDDASFIKT LKRIPLVTSL ASEILAAYLM PPVESGSVDF AEFEPNLVY 

Q9M591 in FASTA format

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