[1]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=cv. Columbia; TISSUE=Leaf; DOI=10.1023/A:1010612506070; PubMed=11442063 [NCBI, ExPASy, EBI, Israel, Japan] Figueroa P., Leon G., Elorza A., Holuigue L., Jordana X.; "Three different genes encode the iron-sulphur subunit of succinate dehydrogenase in Arabidopsis thaliana."; Plant Mol. Biol. 46:241-250(2001).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=cv. Columbia; DOI=10.1093/dnares/5.3.203; PubMed=9734815 [NCBI, ExPASy, EBI, Israel, Japan] Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.; "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."; DNA Res. 5:203-216(1998).
[3]	DEVELOPMENTAL STAGE. DOI=10.1093/pcp/pci218; PubMed=16249327 [NCBI, ExPASy, EBI, Israel, Japan] Elorza A., Roschzttardtz H., Gomez I., Mouras A., Holuigue L., Araya A., Jordana X.; "A nuclear gene for the iron-sulfur subunit of mitochondrial complex II is specifically expressed during Arabidopsis seed development and germination."; Plant Cell Physiol. 47:14-21(2006).

Comments

FUNCTION: Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (By similarity).
CATALYTIC ACTIVITY: Succinate + ubiquinone = fumarate + ubiquinol.
COFACTOR: Binds 1 2Fe-2S cluster (By similarity).
COFACTOR: Binds 1 3Fe-4S cluster (By similarity).
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle .
SUBUNIT: Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b composed of a large and a small subunit (By similarity).
SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side (By similarity).
DEVELOPMENTAL STAGE: Expressed during seed development. Detected during seed maturation and decreased during germination.
SIMILARITY: Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
SIMILARITY: Contains 1 2Fe-2S ferredoxin-type domain.
SIMILARITY: Contains 1 4Fe-4S ferredoxin-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ278912; CAC19857.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB013395; BAB11652.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_680465.2; -.

UniGene

At.45474

3D structure databases

HSSP

P07014; 1NEK. [HSSP ENTRY / PDB]

ModBase

Q9FJP9.

Organism-specific databases

GeneFarm

2172; 175.

TAIR

At5g65165; -.

Ontologies

GO:0005743;	Cellular component: mitochondrial inner membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0051537;	Molecular function: 2 iron, 2 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051538;	Molecular function: 3 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051539;	Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008177;	Molecular function: succinate dehydrogenase (ubiquinone) activity (inferred from electronic annotation from EC).
GO:0022900;	Biological process: electron transport chain (inferred from electronic annotation from UniProtKB-KW).
GO:0006810;	Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
GO:0006099;	Biological process: tricarboxylic acid cycle (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR006058; 2Fe2S_fd_BS.
IPR001450; 4Fe4S_Fe_S_bd.
IPR012675; b-grasp_ferredoxin-like.
IPR001041; Ferredoxin.
IPR012285; Fum_reductase_C.
IPR004489; Succ_DHase/fum_Rdtase_Fe-S.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.20.30; Ferredoxin_fold; 1.
G3DSA:1.10.1060.10; Fum_reductase_C; 1.

TIGRFAMs

TIGR00384; dhsB; 1.

PROSITE

PS00197; 2FE2S_FER_1; 1.
PS51085; 2FE2S_FER_2; FALSE_NEG.
PS00198; 4FE4S_FER_1; 1.
PS51379; 4FE4S_FER_2; 1.
PROSITE graphical view of domain structure (profiles).

Proteomics databases

PRIDE

Q9FJP9; -.

Genome annotation databases

GeneID

836640; -.

GenomeReviews

BA000015_GR; AT5G65165.

KEGG

ath:AT5G65165; -.

NMPDR

fig|3702.1.peg.28594; -.

Other

ProtoNet

Q9FJP9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; 3Fe-4S; 4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Transit peptide; Transport; Tricarboxylic acid cycle.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 22`	`22`	`Mitochondrion (Potential).`
`CHAIN`	`23 309`	`287`	`Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial.`	`PRO_0000247597`
`DOMAIN`	`69 160`	`92`	`2Fe-2S ferredoxin-type.`
`DOMAIN`	`202 232`	`31`	`4Fe-4S ferredoxin-type.`
`METAL`	`120 120`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`125 125`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`128 128`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`140 140`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`212 212`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`215 215`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`218 218`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`222 222`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`270 270`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`276 276`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`280 280`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`BINDING`	`227 227`		`Ubiquinone; shared with DHSD (By similarity).`

Sequence information

Length: 309 AA [This is the length of the unprocessed precursor]

Molecular weight: 34983 Da [This is the MW of the unprocessed precursor]

CRC64: F69189C234D87A1D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSSVLRLLGR RICNPAAEKV RLSSSLSGGG DFPILNGHKA AQDLSKDTLK SQDITKEKEG 

        70         80         90        100        110        120 
QHKEVKKEFK IYRWNPDKPN SKPFLQSFFV DLSSCGPMVL DVLQKIKAED DASLSYRRSC 

       130        140        150        160        170        180 
REGICGSCSM NIDGTNTVAC LKPINPNTSK PTIITPLPHM YVIKDLVVDL TNFYQQYKSM 

       190        200        210        220        230        240 
EPWLKTRKPP KDGREHRQSP KDRKKLDGLY ECILCACCTT SCPSYWWNPE EFPGPAALLQ 

       250        260        270        280        290        300 
AYRWISDSRD EYREERLQAI TESETKVYRC RAIKNCTATC PKGLNPASAI LKMKSKHLLS 


DPLVRTESV

Q9FJP9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools