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[1]
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NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, AND TISSUE SPECIFICITY.
TISSUE=Oocyte;
DOI=10.1042/BJ20021243; PubMed=12374568 [NCBI, ExPASy, EBI, Israel, Japan]
Andersen C.B.,
Sakaue H.,
Nedachi T.,
Kovacina K.S.,
Clayberger C.,
Conti M.,
Roth R.A.;
"Protein kinase B/Akt is essential for the insulin- but not progesterone-stimulated resumption of meiosis in Xenopus oocytes.";
Biochem. J. 369:227-238(2003).
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- FUNCTION: General protein kinase capable of phosphorylating several known proteins. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase (By similarity). Required for insulin-stimulated meiotic reinitiation during oocyte maturation.
- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
- ENZYME REGULATION: Activated in response to insulin. Three specific sites, one in the kinase domain (Thr-309) and the two other ones in the C-terminal regulatory region (Ser-474 and Tyr-475), need to be phosphorylated for its full activation.
- SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1) (By similarity).
- TISSUE SPECIFICITY: Expressed in the oocyte.
- DOMAIN: Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
- SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
- SIMILARITY: Contains 1 AGC-kinase C-terminal domain.
- SIMILARITY: Contains 1 PH domain.
- SIMILARITY: Contains 1 protein kinase domain.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 481 AA [This is the length of the unprocessed precursor] |
Molecular weight: 56042 Da [This is the MW of the unprocessed precursor] |
CRC64: FF56CFB9A6454303 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MNEVAIVKEG WLHKRGEYIK TWRPRYFLLK SDGTFIGYKE RPQDVDQLET PLNNFSVAKC
70 80 90 100 110 120
QLMKTERPKP NTFIIRCLQW TTVIERTFHV DSPEEREEWI QVIQHVADNL KKQEEEMMEV
130 140 150 160 170 180
RSGDSPSDNS GAEEMEVSHS KPKHKVTMNE FEYLKLLGKG TFGKVILVKE KATGRYYAMK
190 200 210 220 230 240
ILKKEVIVAK DEVAHTLTEN RVLQNSRHPF LTALKYSFQT HDRLCFVMEY ANGGELFFHL
250 260 270 280 290 300
SRERIFSEDR ARFYGAEIVS ALDYLHSEKN VVYRDLKLEN LMLDKDGHIK ITDFGLCKEG
310 320 330 340 350 360
IKDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM YEMMCGRLPF YNQDHEKLFE
370 380 390 400 410 420
LILMEEIRFP RTLLPEAKSL LSGLLKKDPK QRLGGGPDDA KEIMQHKFFA GIVWQDVYEK
430 440 450 460 470 480
KLVPPFKPQV TSETDTRYFD EEFTAQMITI TPPDQDDNFE FVDNERRPHF PQFSYSASGN
A
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Q98TY9 in FASTA format |
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