ID   ORF2A_CMVB              Reviewed;         857 AA.
AC   Q66136;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-NOV-2008, entry version 38.
DE   RecName: Full=RNA-directed RNA polymerase 2A;
DE            Short=protein 2A;
DE            EC=2.7.7.48;
GN   Name=RNA2; ORFNames=ORF2a;
OS   Cucumber mosaic virus (strain B) (CMV).
OC   Viruses; ssRNA positive-strand viruses, no DNA stage; Bromoviridae;
OC   Cucumovirus.
OX   NCBI_TaxID=117119;
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=3562; Spinacia oleracea (Spinach).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Jang H., Choi Y., Kim C.;
RL   Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-directed RNA polymerase.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- MISCELLANEOUS: The virus has a tripartite genome (RNA1, RNA2 and
CC       RNA3) and an encapsidated subgenomic RNA (RNA4) from which the
CC       coat protein is expressed. Virions are icosahedral, not enveloped,
CC       with 32 capsomers per nucleocapsid.
CC   -!- SIMILARITY: Belongs to the cucumoviruses/bromoviruses 2A family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; U59740; AAB03187.1; -; mRNA.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR001788; RNA-dep_RNA_pol_vir-typ.
DR   InterPro; IPR007094; RNA_pol_PSvir.
DR   Pfam; PF00978; RdRP_2; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   2: Evidence at transcript level;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA replication;
KW   RNA-directed RNA polymerase; Transferase.
FT   CHAIN         1    857       RNA-directed RNA polymerase 2A.
FT                                /FTId=PRO_0000083272.
FT   DOMAIN      511    624       RdRp catalytic.
SQ   SEQUENCE   857 AA;  96787 MW;  1A73576286E29547 CRC64;
     MAFPAPAFSL ANLLNGSYGV DTPEDMERLR SEQREEAAAA CRNYRPLPAV DVSESVTEDA
     HSLQTPDGAP AEAVSDEFVT YGAEDYLEKS DDELLVAFET MVKPMRIGQL WCPAFNKCSF
     ISSIAMARAL LLAPRTSHRT MKCFEDLVAA IYTKSDFYYS EECEADDVQM DISSRDVPGY
     SFEPWSRTSG FEPPPICEAC DMIMYQCPCF DFNALKKPCA ERTFADDYVI EGLDGVVDNA
     TLLSNLGPFL VPVKCQYEKC PTPTIAIPPN LNRATDRVDI NLVQSICDST LPTHSNYDDS
     FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK
     RNADVPELGD SVNLSRLSKA VAERFFISYI NGNSLASSNF VNVVSNFHDY MEKWKSSGLS
     YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKSITS QFSPLFTALF
     ERFQRCLRER IILPVGKISS LEMAGFDVKN KYCLEIDLSK FDKSQGEFHL LIQEHILNGL
     GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAF TYFGNTIVTM AEFAWCYDTD
     QFEKLLFSGD DSLGFSQLPP VGDSSKFTTL YNMEAKVMEP SVPYICSKFL LSDEFGNTFS
     VPDPLREVQR LGTKKIPYSD NDEFLFAHFM QFVDRLKFLD RMSQSCIDQL SIFFELKYKK
     SGEEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFC ISEFRVERVN SNKQRKKHGI
     ERRCNDKRRT PTGSYGGGEE AETKVSQTES TGTRSQKSQR ESAFKSQTVP LPTVLSSGRS
     GTDRVIPPCE RGEGTRA
//