Cucumis sativus (Cucumber) [TaxID: 3659]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Spinacia oleracea (Spinach) [TaxID: 3562]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
PubMed=7561763 [NCBI, ExPASy, EBI, Israel, Japan]
McGarvey P.B., Tousignant M., Geletka L., Cellini F., Kaper J.M.;
"The complete sequence of a cucumber mosaic virus from Ixora that is deficient in the replication of satellite RNAs.";
J. Gen. Virol. 76:2257-2270(1995).

Comments

FUNCTION: RNA-directed RNA polymerase.
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
MISCELLANEOUS: The virus has a tripartite genome (RNA1, RNA2 and RNA3) and an encapsidated subgenomic RNA (RNA4) from which the coat protein is expressed. Virions are icosahedral, not enveloped, with 32 capsomers per nucleocapsid.
SIMILARITY: Belongs to the cucumoviruses/bromoviruses 2A family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U20218; AAC54616.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A71392; A71392.

3D structure databases

ModBase

Q66117.

Ontologies

GO:0000166;	Molecular function: nucleotide binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006410;	Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001788; RNA-dep_RNA_pol_vir-typ.
IPR007094; RNA_pol_PSvir.
Graphical view of domain structure.

Pfam

PF00978; RdRP_2; 1.
Pfam graphical view of domain structure.

PROSITE

PS50507; RDRP_SSRNA_POS; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 858`	`858`	`RNA-directed RNA polymerase 2A.`	`PRO_0000083275`
`DOMAIN`	`511 624`	`114`	`RdRp catalytic.`

Sequence information

Length: 858 AA [This is the length of the unprocessed precursor]

Molecular weight: 97045 Da [This is the MW of the unprocessed precursor]

CRC64: AE0151CA566B4005 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAFPAPAFSL ANLLNGTYGV DTPEEVERVR SEQREEAAAA CRNYKPLPAV DVGASVTEDA 

        70         80         90        100        110        120 
HSLRTPDGAP SEEVSVEFVT YGAEDYLEKS DDELFVAFET MVKPMCIGQL WCPAFNKCSF 

       130        140        150        160        170        180 
ISSIAMARAL LLVPNTPNRT MKCFEDLVAA IYTKSDFYYD DECEADDVQI NISSRDVPGY 

       190        200        210        220        230        240 
SFEPWSRTSG FEPPPICEAC NMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVDNA 

       250        260        270        280        290        300 
TLLSNLGPFL VPVKCQYKQY PTPTVAIPPS LNRATDRVDI NLVQSICDST LPTHSNYDDS 

       310        320        330        340        350        360 
FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK 

       370        380        390        400        410        420 
RNADVPELGD SVNLPRLSKA VAERFFIAYI NGNSLATSNF VNVVSNFHDY MEKWKSSGLS 

       430        440        450        460        470        480 
YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKGITS QFSPLFTALF 

       490        500        510        520        530        540 
ERFQRCLRER VILPVGKISS LEMSGFDVKN KYCLEIDLSK FDKSQGEFHL MIQEHILNGL 

       550        560        570        580        590        600 
GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAF TYFGNTIVTM AEFAWCYDTD 

       610        620        630        640        650        660 
QFEKLLFPGD DSLGFSVLPP VGDPSKFTTL FNMEAKVMEP AVPYICSKFL LSDEFGNTFS 

       670        680        690        700        710        720 
VPDPLREVQR LGTKKIPYSD NDEFLFAHFM SFVDRLKFLD RMTQSCIDQL SLFFELKYRK 

       730        740        750        760        770        780 
SGAEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFS CVELRIERSS FIKQRKKKDG 

       790        800        810        820        830        840 
IERRRNDKRR TPTSPHGGGE ETETKVSQEE STGTMLQKSQ RESAFKSQTI PFPTVLSSRR 

       850 
FGIDRDVPPR ECGGIVRV

Q66117 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools