NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
STRAIN=Sprague-Dawley;
DOI=10.1074/jbc.270.19.11595; PubMed=7744798 [NCBI, ExPASy, EBI, Israel, Japan]
Bhattacharyya K.K., Brake P.B., Eltom S.E., Otto S.A., Jefcoate C.R.;
"Identification of a rat adrenal cytochrome P450 active in polycyclic hydrocarbon metabolism as rat CYP1B1. Demonstration of a unique tissue-specific pattern of hormonal and aryl hydrocarbon receptor-linked regulation.";
J. Biol. Chem. 270:11595-11602(1995).

[2]

NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Sprague-Dawley;
TISSUE=Liver;
DOI=10.1093/carcin/16.6.1319; PubMed=7788849 [NCBI, ExPASy, EBI, Israel, Japan]
Walker N.J., Gastel J.A., Costa L.T., Clark G.C., Lucier G.W., Sutter T.R.;
"Rat CYP1B1: an adrenal cytochrome P450 that exhibits sex-dependent expression in livers and kidneys of TCDD-treated animals.";
Carcinogenesis 16:1319-1327(1995).

Comments

FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
CATALYTIC ACTIVITY: RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
COFACTOR: Heme group (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
INDUCTION: By polycyclic aromatic hydrocarbons (PAH) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X83867; CAA58748.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U09540; AAA79864.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

I48130; I48130.

NP_037072.1; -.

3D structure databases

HSSP

P00179; 1DT6. [HSSP ENTRY / PDB]

ModBase

Q64678.

Organism-specific databases

RGD

2460; Cyp1b1.

Gene expression databases

ArrayExpress

Q64678; -.

GermOnline

ENSRNOG00000040287; Rattus norvegicus.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005792;	Cellular component: microsome (inferred from electronic annotation from UniProtKB-KW).
GO:0050381;	Molecular function: unspecific monooxygenase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

Genome annotation databases

Ensembl

ENSRNOG00000040287; Rattus norvegicus. [Contig view]

GeneID

25426; -.

KEGG

rno:25426; -.

Phylogenomic databases

HOVERGEN

Q64678; -.

Other

NextBio

606603; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 543`	`543`	`Cytochrome P450 1B1.`	`PRO_0000051662`
`METAL`	`470 470`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 543 AA [This is the length of the unprocessed precursor]

Molecular weight: 60557 Da [This is the MW of the unprocessed precursor]

CRC64: 8D144B0000D5F095 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MATSLSADSP QQLSSLSTQQ TILLLLVSVL AIVHLGQWLL RQWRRKPWSS PPGPFPWPLI 

        70         80         90        100        110        120 
GNAASVGRAS HLYFARLARR YGDVFQIRLG SCPVVVLNGE SAIHQALVQQ GGVFADRPPF 

       130        140        150        160        170        180 
ASFRVVSGGR SLAFGHYSER WKERRRAAYG TMRAFSTRHP RSRGLLEGHA LGEARELVAV 

       190        200        210        220        230        240 
LVRRCAGGAC LDPTQPIIVA VANVMSAVCF GCRYNHDDAE FLELLSHNEE FGRTVGAGSL 

       250        260        270        280        290        300 
VDVMPWLQLF PNPVRTIFRE FEQINRNFSN FVLDKFLRHR ESLVPGAAPR DMMDAFILSA 

       310        320        330        340        350        360 
EKKATGDPGD SPSGLDLEDV PATITDIFGA SQDTLSTALL WLLILFTRYP DVQARVQAEL 

       370        380        390        400        410        420 
DQVVGRDRLP CMSDQPNLPY VMAFLYESMR FTSFLPVTLP HATTANTFVL GYYIPKNTVV 

       430        440        450        460        470        480 
FVNQWSVNHD PAKWSNPEDF DPARFLDKDG FINKALASSV MIFSVGKRRC IGEELSKTLL 

       490        500        510        520        530        540 
FLFISILAHQ CNFKANQNEP SNMSFSYGLS IKPKSFKIHV SLRESMKLLD SAVEKLQAEE 


ACQ

Q64678 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools