[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Brain cortex; Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.; "Isolation and characterization of cDNA for macaque neurological disease genes."; Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity).
CATALYTIC ACTIVITY: NADH + 2 ferricytochrome b5 = NAD⁺ + H⁺ + 2 ferrocytochrome b5.
COFACTOR: FAD (By similarity).
SUBUNIT: Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2 (By similarity).
SUBCELLULAR LOCATION: Isoform 1: Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side (By similarity). Mitochondrion outer membrane; Lipid-anchor; Cytoplasmic side (By similarity).
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm. Note=Produces the soluble form found in erythrocytes (By similarity).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative promoter usage.

Name	1
Synonyms	M
Isoform ID	Q60HG4-1
This is the isoform sequence displayed in this entry.

Name	2
Synonyms	S
Isoform ID	Q60HG4-2
Features which should be applied to build the isoform sequence: VSP_013359.

SIMILARITY: Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
SIMILARITY: Contains 1 FAD-binding FR-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB125163; BAD51951.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

SMR

Q60HG4; 30-300, 31-301.

ModBase

Q60HG4.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005741;	Cellular component: mitochondrial outer membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0004128;	Molecular function: cytochrome-b5 reductase activity (inferred from electronic annotation from EC).
GO:0006695;	Biological process: cholesterol biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001834; Cyt_B5_reductase.
IPR001709; FPN_cyt_redctse.
IPR008333; OxRdtase_FAD-bd.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.

Pfam

PF00970; FAD_binding_6; 1.
PF00175; NAD_binding_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00406; CYTB5RDTASE.
PR00371; FPNCR.

PROSITE

PS51384; FAD_FR; 1.
PROSITE graphical view of domain structure (profiles).

Phylogenomic databases

HOVERGEN

Q60HG4; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Acetylation; Alternative promoter usage; Cholesterol biosynthesis; Cytoplasm; Endoplasmic reticulum; FAD; Flavoprotein; Lipid synthesis; Lipoprotein; Membrane; Mitochondrion; Mitochondrion outer membrane; Myristate; NAD; Oxidoreductase; Phosphoprotein; Steroid biosynthesis; Sterol biosynthesis.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 301`	`300`	`NADH-cytochrome b5 reductase 3 membrane-bound form.`	`PRO_0000019395`
`CHAIN`	`27 301`	`275`	`NADH-cytochrome b5 reductase 3 soluble form.`	`PRO_0000019397`
`DOMAIN`	`40 152`	`113`	`FAD-binding FR-type.`
`NP_BIND`	`132 147`	`16`	`FAD (By similarity).`
`NP_BIND`	`171 206`	`36`	`FAD (By similarity).`
`MOD_RES`	`42 42`		`N6-acetyllysine (By similarity).`
`MOD_RES`	`120 120`		`N6-acetyllysine (By similarity).`
`MOD_RES`	`130 130`		`Phosphotyrosine (By similarity).`
`LIPID`	`2 2`		`N-myristoyl glycine (By similarity).`
`VAR_SEQ`	`1 23`		`Missing (in isoform 2).`	`VSP_013359`

Sequence information

Length: 301 AA [This is the length of the unprocessed precursor]

Molecular weight: 34142 Da [This is the MW of the unprocessed precursor]

CRC64: FEAF8CF96B88DDEF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGAQLSTLGH VVLSPVWFLY SLLMKLFRCS TPAITLESPD IKYSLRLIDR EIISHDTRRF 

        70         80         90        100        110        120 
RFALPSPQHI LGLPVGQHIY LSARIDGNLV IRPYTPVSSD DDKGFVDLVI KVYFKDTHPK 

       130        140        150        160        170        180 
FPAGGKMSQY LESMQIGDTI EFRGPNGLLV YQGKGKFAIR PDKKSNPVIK TVKSVGMIAG 

       190        200        210        220        230        240 
GTGITPMLQV IRAIMKDPDD HTVCHLLFAN QTEKDILLRP ELEELRNEHS ARFKLWYTLD 

       250        260        270        280        290        300 
RAPEAWDYSQ GFVNEEMIRD HLPPPEEEPL VLMCGPPPMI QYACLPNLDR VGHPKERCFA 


F

Q60HG4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools