[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Spleen; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[2]	FUNCTION, AND INTERACTION WITH BET1; GOSR2 AND STX5. DOI=10.1074/jbc.M007684200; PubMed=11035026 [NCBI, ExPASy, EBI, Israel, Japan] Xu D., Joglekar A.P., Williams A.L., Hay J.C.; "Subunit structure of a mammalian ER/Golgi SNARE complex."; J. Biol. Chem. 275:39631-39639(2000).
[3]	SUBCELLULAR LOCATION. DOI=10.1091/mbc.E03-08-0625; PubMed=14742712 [NCBI, ExPASy, EBI, Israel, Japan] Volchuk A., Ravazzola M., Perrelet A., Eng W.S., Di Liberto M., Varlamov O., Fukasawa M., Engel T., Sollner T.H., Rothman J.E., Orci L.; "Countercurrent distribution of two distinct SNARE complexes mediating transport within the Golgi stack."; Mol. Biol. Cell 15:1506-1518(2004).

Comments

FUNCTION: SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
SUBUNIT: Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B as subunit of either complex.
SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein. Golgi apparatus membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein (Potential). Melanosome (By similarity).
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity).
SIMILARITY: Belongs to the synaptobrevin family.
SIMILARITY: Contains 1 longin domain.
SIMILARITY: Contains 1 v-SNARE coiled-coil homology domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC098725; AAH98725.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI00193522; -.

NP_001020857.1; -.

3D structure databases

SMR

Q4KM74; 1-126, 2-127.

ModBase

Q4KM74.

Organism-specific databases

RGD

1309988; Sec22b.

Gene expression databases

ArrayExpress

Q4KM74; -.

GermOnline

ENSRNOG00000018673; Rattus norvegicus.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0033116;	Cellular component: ER-Golgi intermediate compartment membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0000139;	Cellular component: Golgi membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0042470;	Cellular component: melanosome (inferred from electronic annotation from UniProtKB-SubCell).
GO:0015031;	Biological process: protein transport (inferred from electronic annotation from UniProtKB-KW).
GO:0016192;	Biological process: vesicle-mediated transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR010908; Longin.
IPR001388; Synaptobrevin.
Graphical view of domain structure.

Pfam

PF00957; Synaptobrevin; 1.
Pfam graphical view of domain structure.

PRINTS

PR00219; SYNAPTOBREVN.

PROSITE

PS50859; LONGIN; 1.
PS50892; V_SNARE; 1.
PROSITE graphical view of domain structure (profiles).

Proteomic databases

PRIDE

Q4KM74; -.

Genome annotation databases

Ensembl

ENSRNOG00000018673; Rattus norvegicus. [Contig view]

GeneID

310710; -.

KEGG

rno:310710; -.

Phylogenomic databases

HOVERGEN

Q4KM74; -.

OMA

Q4KM74; NQAKMLF.

Other

662501; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Coiled coil; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; Membrane; Phosphoprotein; Protein transport; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 215`	`214`	`Vesicle-trafficking protein SEC22b.`	`PRO_0000253048`
`TOPO_DOM`	`2 194`	`193`	`Cytoplasmic (Potential).`
`TRANSMEM`	`195 215`	`21`	`Anchor for type IV membrane protein (Potential).`
`DOMAIN`	`6 119`	`114`	`Longin.`
`DOMAIN`	`134 194`	`61`	`v-SNARE coiled-coil homology.`
`MOD_RES`	`35 35`		`Phosphoserine (By similarity).`
`MOD_RES`	`48 48`		`Phosphoserine (By similarity).`
`MOD_RES`	`137 137`		`Phosphoserine (By similarity).`
`MOD_RES`	`168 168`		`Phosphoserine (By similarity).`

Sequence information

Length: 215 AA [This is the length of the unprocessed precursor]

Molecular weight: 24741 Da [This is the MW of the unprocessed precursor]

CRC64: 29B4C55961C5A044 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVLLTMIARV ADGLPLAASM QEDEQSGRDL QQYQSQAKQL FRKLNEQSPT RCTLEAGAMT 

        70         80         90        100        110        120 
FHYIIEQGVC YLVLCEAAFP KKLAFAYLED LHSEFDEQHG KKVPTVSRPY SFIEFDTFIQ 

       130        140        150        160        170        180 
KTKKLYIDSR ARRNLGSINT ELQDVQRIMV ANIEEVLQRG EALSALDSKA NNLSSLSKKY 

       190        200        210 
RQDAKYLNMR STYAKLAAVA VFFIMLIVYV RFWWL

Q4KM74 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools