[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Hereford; TISSUE=Ascending colon; NIH - Mammalian Gene Collection (MGC) project; Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity).
CATALYTIC ACTIVITY: NADH + 2 ferricytochrome b5 = NAD⁺ + H⁺ + 2 ferrocytochrome b5.
COFACTOR: FAD (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein (Potential).
SIMILARITY: Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
SIMILARITY: Contains 1 FAD-binding FR-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC104584; AAI04585.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001029690.1; -.

UniGene

Bt.997

3D structure databases

ModBase

Q3MHW9.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0004128;	Molecular function: cytochrome-b5 reductase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0016126;	Biological process: sterol biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001834; Cyt_B5_reductase.
IPR001709; FPN_cyt_redctse.
IPR008333; OxRdtase_FAD-bd.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.

Pfam

PF00970; FAD_binding_6; 1.
PF00175; NAD_binding_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00406; CYTB5RDTASE.
PR00371; FPNCR.

PROSITE

PS51384; FAD_FR; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSBTAG00000019927; Bos taurus. [Contig view]

GeneID

516287; -.

KEGG

bta:516287; -.

Phylogenomic databases

HOVERGEN

Q3MHW9; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

FAD; Flavoprotein; Lipid synthesis; Membrane; NAD; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 305`	`305`	`NADH-cytochrome b5 reductase 1.`	`PRO_0000287544`
`TRANSMEM`	`8 28`	`21`	`Potential.`
`DOMAIN`	`44 156`	`113`	`FAD-binding FR-type.`
`NP_BIND`	`136 166`	`31`	`FAD (By similarity).`
`NP_BIND`	`175 210`	`36`	`FAD (By similarity).`

Sequence information

Length: 305 AA [This is the length of the unprocessed precursor]

Molecular weight: 33861 Da [This is the MW of the unprocessed precursor]

CRC64: BC12CA38311828F4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGLQPSPVLL ASLGVGLLTL LGVALGAYLV RRSRRPPVTL LDPNEKYQLR LLDKTTVNHN 

        70         80         90        100        110        120 
TKRFRFALPT AHHVLGLPVG KHVYLSARID GSLVIRPYTP VTSDEDQGYV DLVIKVYLKG 

       130        140        150        160        170        180 
VHPKFPEGGK MSQYLDSLKI GDVVEFRGPS GLLTYAGKGK FNIQPNKKAP PEARVARNLG 

       190        200        210        220        230        240 
MIAGGTGITP MLQLIRAILK DPEDPTQCFL LFANQTEKDI ILREDLEELQ ARHPGRFKLW 

       250        260        270        280        290        300 
FTLDHPPEGW AYSKGFVSVD MIREHLPAPG EDVLLLLCGP PPMVQLACHP SLDKLGYSPK 


MRFTY

Q3MHW9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools