[1]	NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY. TISSUE=Brain; PubMed=2472388 [NCBI, ExPASy, EBI, Israel, Japan] Elferink L.A., Trimble W.S., Scheller R.H.; "Two vesicle-associated membrane protein genes are differentially expressed in the rat central nervous system."; J. Biol. Chem. 264:11061-11064(1989).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Lung; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[3]	PROTEIN SEQUENCE OF 32-47 AND 60-83, AND MASS SPECTROMETRY. STRAIN=Sprague-Dawley; TISSUE=Hippocampus; Lubec G., Chen W.-Q.; Submitted (JAN-2009) to UniProtKB.
[4]	X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 1-97 IN COMPLEX WITH STX1A AND SNAP25. DOI=10.1038/26412; PubMed=9759724 [NCBI, ExPASy, EBI, Israel, Japan] Sutton R.B., Fasshauer D., Jahn R., Brunger A.T.; "Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution."; Nature 395:347-353(1998).
[5]	X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 30-94 IN COMPLEX WITH STX1A; CPLX1 AND SNAP25, AND STRUCTURE BY NMR. DOI=10.1016/S0896-6273(02)00583-4; PubMed=11832227 [NCBI, ExPASy, EBI, Israel, Japan] Chen X., Tomchick D.R., Kovrigin E., Arac D., Machius M., Suedhof T.C., Rizo J.; "Three-dimensional structure of the complexin/SNARE complex."; Neuron 33:397-409(2002).
[6]	X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 29-90 IN COMPLEX WITH STX1A AND SNAP25. DOI=10.1074/jbc.M211889200; PubMed=12496247 [NCBI, ExPASy, EBI, Israel, Japan] Ernst J.A., Brunger A.T.; "High resolution structure, stability, and synaptotagmin binding of a truncated neuronal SNARE complex."; J. Biol. Chem. 278:8630-8636(2003).

Comments

FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity).
SUBUNIT: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with VAPA and VAPB (By similarity).
INTERACTION:
O35394:Rabac1; NbExp=1; IntAct=EBI-520880, EBI-2028510;
P32851:Stx1a; NbExp=2; IntAct=EBI-520880, EBI-539720;
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Note=Neuronal synaptic vesicles.
TISSUE SPECIFICITY: Nervous system specific. A higher level expression is seen in the brain as compared to the spinal cord.
SIMILARITY: Belongs to the synaptobrevin family.
SIMILARITY: Contains 1 v-SNARE coiled-coil homology domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M24105; AAA42321.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC074003; AAH74003.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI00204447; -.

B34288; B34288.

NP_036795.1; -.

3D structure databases

PDB

1KIL; X-ray; 2.30 A; A=28-92.	[ExPASy / RCSB / EBI]
1N7S; X-ray; 1.45 A; A=28-89.	[ExPASy / RCSB / EBI]
1SFC; X-ray; 2.40 A; A/E/I=1-96.	[ExPASy / RCSB / EBI]
2BU0; Model; -; A=25-88.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1KIL; -.
1N7S; -.
1SFC; -.
2BU0; -.

ModBase

P63045.

Protein-protein interaction databases

IntAct

P63045; 13.

PTM databases

PhosphoSite

P63045; -.

Organism-specific databases

RGD

3949; Vamp2.

Gene expression databases

ArrayExpress

P63045; -.

GermOnline

ENSRNOG00000006989; Rattus norvegicus.

Ontologies

GO:0030054;	Cellular component: cell junction (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005792;	Cellular component: microsome (inferred from direct assay from RGD).
GO:0030672;	Cellular component: synaptic vesicle membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0070044;	Cellular component: synaptobrevin 2-SNAP-25-syntaxin-1a complex (inferred from direct assay from MGI).
GO:0070032;	Cellular component: synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex (inferred from direct assay from MGI).
GO:0019717;	Cellular component: synaptosome (inferred from direct assay from RGD).
GO:0017022;	Molecular function: myosin binding (inferred from physical interaction from RGD).
GO:0032403;	Molecular function: protein complex binding (inferred from physical interaction from RGD).
GO:0017075;	Molecular function: syntaxin-1 binding (inferred from direct assay from MGI).
GO:0006461;	Biological process: protein complex assembly (inferred from direct assay from RGD).
GO:0015031;	Biological process: protein transport (inferred from mutant phenotype from RGD).
GO:0017157;	Biological process: regulation of exocytosis (traceable author statement from UniProtKB).
GO:0009749;	Biological process: response to glucose stimulus (inferred from direct assay from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR001388; Synaptobrevin.
IPR016444; Synaptobrevin_met/fun.
Graphical view of domain structure.

Pfam

PF00957; Synaptobrevin; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF005409; Synaptobrevin_euk; 1.

PRINTS

PR00219; SYNAPTOBREVN.

ProDom

PD001229; Synaptobrevin; 1.
[Domain structure / List of seq. sharing at least 1 domain]

PROSITE

PS00417; SYNAPTOBREVIN; 1.
PS50892; V_SNARE; 1.
PROSITE graphical view of domain structure (profiles).

Proteomic databases

PRIDE

P63045; -.

Genome annotation databases

Ensembl

ENSRNOG00000006989; Rattus norvegicus. [Contig view]

GeneID

24803; -.

KEGG

rno:24803; -.

Phylogenomic databases

HOVERGEN

P63045; -.

Other

604468; -.

P63045; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Acetylation; Cell junction; Coiled coil; Cytoplasmic vesicle; Direct protein sequencing; Membrane; Phosphoprotein; Synapse; Synaptosome; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 116`	`115`	`Vesicle-associated membrane protein 2.`	`PRO_0000206726`
`TOPO_DOM`	`2 94`	`93`	`Cytoplasmic (Potential).`
`TRANSMEM`	`95 114`	`20`	`Anchor for type IV membrane protein (Potential).`
`TOPO_DOM`	`115 116`	`2`	`Vesicular (Potential).`
`DOMAIN`	`31 91`	`61`	`v-SNARE coiled-coil homology.`
`MOD_RES`	`2 2`		`N-acetylserine (By similarity).`
`MOD_RES`	`75 75`		`Phosphoserine (By similarity).`
`HELIX`	`29 88`	`60`

Sequence information

Length: 116 AA [This is the length of the unprocessed precursor]

Molecular weight: 12691 Da [This is the MW of the unprocessed precursor]

CRC64: 4A0D0D56B5409D0A [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSATAATVPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL 

        70         80         90        100        110 
SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG VICAIILIII IVYFST

P63045 in FASTA format

View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools