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UniProtKB/Swiss-Prot entry P63027


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name VAMP2_HUMAN
Primary accession number P63027
Secondary accession number P19065
Integrated into Swiss-Prot on August 31, 2004
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    June 16, 2009 (Entry version 52)
Name and origin of the protein
Protein name Vesicle-associated membrane protein 2
Synonyms VAMP-2
Synaptobrevin-2
Gene name
Name: VAMP2
Synonyms: SYB2
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1976629 [NCBI, ExPASy, EBI, Israel, Japan]
Archer B.T. III, Oezcelik T., Jahn R., Francke U., Suedhof T.C.;
"Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2.";
J. Biol. Chem. 265:17267-17273(1990).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Peripheral blood;
Nabokina S.M., Lazo P.A., Mollinedo F.;
"Expression of VAMP genes in human neutrophils.";
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
TISSUE=Blood;
Taruscio D., Zoraqi K.G., Falbo V.;
"Genomic structure of human SYB2 gene.";
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
[4]
TISSUE SPECIFICITY.
PubMed=8760387 [NCBI, ExPASy, EBI, Israel, Japan]
Jagadish M.N., Fernandez C.S., Hewish D.R., Macaulay S.L., Gough K.H., Grusovin J., Verkuylen A., Cosgrove L., Alafaci A., Frenkel M.J., Ward C.W.;
"Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2.";
Biochem. J. 317:945-954(1996).
[5]
TOPOLOGY.
PubMed=7835332 [NCBI, ExPASy, EBI, Israel, Japan]
Kutay U., Ahnert-Hilger G., Hartmann E., Wiedenmann B., Rapoport T.A.;
"Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane.";
EMBO J. 14:217-223(1995).
[6]
IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
Colinge J., Superti-Furga G., Bennett K.L.;
Submitted (OCT-2008) to UniProtKB.
[7]
X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 34-89 IN COMPLEX WITH BOTB.
DOI=10.1038/77997; PubMed=10932255 [NCBI, ExPASy, EBI, Israel, Japan]
Hanson M.A., Stevens R.C.;
"Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 A resolution.";
Nat. Struct. Biol. 7:687-692(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M36205; AAA60604.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M36201; AAA60604.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M36202; AAA60604.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M36203; AAA60604.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M36204; AAA60604.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ225044; CAA12385.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF135372; AAF15551.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00553138; -.
PIR B38315; B38315.
UniGene Hs.25348
3D structure databases
SMR P63027; 25-93.
DisProt DP00069; -.
ModBase P63027.
Protein-protein interaction databases
IntAct P63027; 2.
Protein family/group databases
TCDB 1.F.1.1.1; synaptosomal vesicle fusion pore (SVF-Pore) family.
PTM databases
PhosphoSite P63027; -.
Enzyme and pathway databases
Pathway_Interaction_DB botulinumtoxinpathway; Effects of Botulinum toxin.
insulin_glucose_pathway; Insulin-mediated glucose transport.
Reactome REACT_11123; Membrane Trafficking.
REACT_11184; Botulinum neurotoxicity.
REACT_13685; Synaptic Transmission.
Organism-specific databases
GeneCards GC17M007987; -.
H-InvDB HIX0020382; -.
HGNC HGNC:12643; VAMP2.
GenAtlas VAMP2.
MIM 185881; gene. [NCBI / EBI]
PharmGKB PA37267; -.
Gene expression databases
ArrayExpress P63027; -.
CleanEx HS_VAMP2; -.
GermOnline ENSG00000179036; Homo sapiens.
Ontologies
GO
GO:0030054; Cellular component: cell junction (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005887; Cellular component: integral to plasma membrane (traceable author statement from ProtInc).
GO:0030141; Cellular component: secretory granule (inferred from electronic annotation from UniProtKB-SubCell).
GO:0030672; Cellular component: synaptic vesicle membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0019717; Cellular component: synaptosome (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from UniProtKB).
GO:0016192; Biological process: vesicle-mediated transport (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001388; Synaptobrevin.
IPR016444; Synaptobrevin_met/fun.
Graphical view of domain structure.
Pfam PF00957; Synaptobrevin; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF005409; Synaptobrevin_euk; 1.
PRINTS PR00219; SYNAPTOBREVN.
ProDom PD001229; Synaptobrevin; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00417; SYNAPTOBREVIN; 1.
PS50892; V_SNARE; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE P63027; -.
Genome annotation databases
Ensembl ENSG00000179036; Homo sapiens. [Contig view]
Phylogenomic databases
HOGENOM P63027; -.
HOVERGEN P63027; -.
Other
DrugBank DB00042; Botulinum Toxin Type B.
SOURCE VAMP2; Homo sapiens.
ProtoNet P63027.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Cell junction; Coiled coil; Cytoplasmic vesicle; Membrane; Phosphoprotein; Synapse; Synaptosome; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed (By similarity). 
CHAIN   2   116  115     Vesicle-associated membrane protein 2. PRO_0000206723
TOPO_DOM   2    94  93     Cytoplasmic (Potential). 
TRANSMEM   95   114  20     Anchor for type IV membrane protein (Potential). 
TOPO_DOM   115   116  2     Vesicular (Potential). 
DOMAIN   31    91  61     v-SNARE coiled-coil homology. 
MOD_RES   2     2        N-acetylserine (By similarity). 
MOD_RES   75    75        Phosphoserine (By similarity). 
Sequence information
Length: 116 AA [This is the length of the unprocessed precursor] Molecular weight: 12649 Da [This is the MW of the unprocessed precursor] CRC64: 98C679C4F6F1B5A8 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL 

        70         80         90        100        110 
SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG VICAIILIII IVYFSS 

P63027 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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