3-beta-hydroxy-Delta(5)-steroid dehydrogenase
     (EC 1.1.1.145)
     (3-beta-hydroxy-5-ene steroid dehydrogenase)
     (Progesterone reductase)
Steroid Delta-isomerase
     (EC 5.3.3.1)
     (Delta-5-3-ketosteroid isomerase)

Gene name

Name:

HSD3B1

From

Macaca mulatta (Rhesus macaque)

[TaxID: 9544]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.

Protein existence

2: Evidence at transcript level;

References

[1]

NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Ovary;
DOI=10.1016/0303-7207(91)90224-G; PubMed=2050270 [NCBI, ExPASy, EBI, Israel, Japan]
Simard J., Melner M.H., Breton N., Low K.G., Zhao H.-F., Periman L.M., Labrie F.;
"Characterization of macaque 3 beta-hydroxy-5-ene steroid dehydrogenase/delta 5-delta 4 isomerase: structure and expression in steroidogenic and peripheral tissues in primate.";
Mol. Cell. Endocrinol. 75:101-110(1991).

Comments

FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
CATALYTIC ACTIVITY: A 3-beta-hydroxy-Delta⁵-steroid + NAD⁺ = a 3-oxo-Delta⁵-steroid + NADH.
CATALYTIC ACTIVITY: A 3-oxo-Delta⁵-steroid = a 3-oxo-Delta⁴-steroid.
PATHWAY: Lipid metabolism; steroid biosynthesis .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
TISSUE SPECIFICITY: Adrenal glands, testes and ovaries.
SIMILARITY: Belongs to the 3-beta-HSD family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M67467; AAA36847.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A54325; A54325.

RefSeq

XP_001113717.1; -.
XP_001113744.1; -.
XP_001113769.1; -.

UniGene

Mmu.15109

3D structure databases

ModBase

P27365.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0031966;	Cellular component: mitochondrial membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0003854;	Molecular function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity (inferred from electronic annotation from InterPro).
GO:0005488;	Molecular function: binding (inferred from electronic annotation from InterPro).
GO:0004769;	Molecular function: steroid delta-isomerase activity (inferred from electronic annotation from EC).
GO:0006700;	Biological process: C21-steroid hormone biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR002225; 3Beta_OHSteriod_DHase/Estase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF01073; 3Beta_HSD; 1.
Pfam graphical view of domain structure.

ProtoNet

P27365.

Genome annotation databases

Ensembl

ENSMMUG00000016568; Macaca mulatta. [Contig view]

GeneID

712686; -.

Phylogenomic databases

HOVERGEN

P27365; -.

Other

LinkHub

P27365; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Isomerase; Membrane; Mitochondrion; Multifunctional enzyme; NAD; Oxidoreductase; Steroidogenesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 373`	`372`	`3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1.`	`PRO_0000087776`
`TRANSMEM`	`288 308`	`21`	`Potential.`

Sequence information

Length: 373 AA [This is the length of the unprocessed precursor]

Molecular weight: 42005 Da [This is the MW of the unprocessed precursor]

CRC64: 9B6C172F319B5BB9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTGWSCLVTG AGGFLGQRIV RLLVEEKELK EIRVLDKAFR PELREEFSKL QNKTKLTVLE 

        70         80         90        100        110        120 
GDILDEPFLK RACQDVSVVI HTACIIDVFG VTHRESIMNV NVKGTQLLLE ACVQASVPVF 

       130        140        150        160        170        180 
IYTSTLEVAG PNSYKEIIQN GHEEEPLENT WPAPYPYSKK LAEKAVLAAN GWTLKNGGTL 

       190        200        210        220        230        240 
YTCALRPMYI YGEGGPFLSA SINEALNNNG ILSSVGKFST VNPVYVGNVA WAHILALRAL 

       250        260        270        280        290        300 
RDPKKAPSVQ GQFYYISDDT PHQSYDNLNY ILSKEFGLCL DSRWSLPLAL MYWIGFLLEV 

       310        320        330        340        350        360 
VSFLLSPVYS YQPPFNRHTV TLSNSVFTFS YKKAQRDLAY KPLYSWEEAK QKTVEWVGSL 

       370 
VDRHKETLKS KTQ

P27365 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools