[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=1774279 [NCBI, ExPASy, EBI, Israel, Japan] Lyerly D.M., Barroso L.A., Wilkins T.D.; "Identification of the latex test-reactive protein of Clostridium difficile as glutamate dehydrogenase."; J. Clin. Microbiol. 29:2639-2642(1991).

Comments

CATALYTIC ACTIVITY: L-glutamate + H₂O + NAD⁺ = 2-oxoglutarate + NH₃ + NADH.
SUBUNIT: Homotetramer.
SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M65250; AAA62756.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S28829; S28829.

3D structure databases

HSSP

O74024; 1EUZ. [HSSP ENTRY / PDB]

ModBase

P27346.

Ontologies

GO:0005488;	Molecular function: binding (inferred from electronic annotation from InterPro).
GO:0004352;	Molecular function: glutamate dehydrogenase activity (inferred from electronic annotation from EC).
GO:0006520;	Biological process: amino acid metabolic process (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR006095; Glu/Leu/Phe/Val_DHase.
IPR006096; Glu/Leu/Phe/Val_DHase_C.
IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
IPR014362; Glu_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR11606:SF2; GLFV_DH; 1.

Pfam

PF00208; ELFV_dehydrog; 1.
PF02812; ELFV_dehydrog_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000185; Glu_DH; 1.

PRINTS

PR00082; GLFDHDRGNASE.

PROSITE

PS00074; GLFV_DEHYDROGENASE; 1.

ProtoNet

P27346.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 421`	`421`	`NAD-specific glutamate dehydrogenase.`	`PRO_0000182737`
`ACT_SITE`	`107 107`		`By similarity.`

Sequence information

Length: 421 AA [This is the length of the unprocessed precursor]

Molecular weight: 46016 Da [This is the MW of the unprocessed precursor]

CRC64: 31F0A09802F4AD94 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSGKDVNVFE MAQSQVKNAC DKLGMEPAVY ELLKEPMRVI EVSIPVKMDD GSIKTFKGFR 

        70         80         90        100        110        120 
SQHNDAVGPT KGGIRFHQNV SRDEVKALSI WMTFKCSVTG IPYGGGKGGI IVDPSTLSQG 

       130        140        150        160        170        180 
ELERLSRGYI DGIYKLIGEK VDVPAPDVNT NGQIMSWMVD EYNKLTGQSS IGVITGKPVE 

       190        200        210        220        230        240 
FGGSLGRTAA TGFGVAVTAR EAAAKLGIDM KKAKIAVQGI GNVGSYTVLN CEKLGGTVVA 

       250        260        270        280        290        300 
MAEWCKSEGS YAIYNENGLD GQAMLDYMKE HGNLLNFPGA KRISLEEFWA SDVDIVIPAA 

       310        320        330        340        350        360 
LENSITKEVA ESIKAKLVCE AANGPTTPEA DEVFAERGIV LTPDILTNAG GVTVSYFEWV 

       370        380        390        400        410        420 
QNLYGYYWSE EEVEQKEEIA MVKAFESIWK IKEEYNVTMR EAAYMHSIKK VAEAMKLRGW 


Y

P27346 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools