RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Human parainfluenza 2 virus (strain Toshiba) (HPIV-2)

[TaxID: 11214]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Rubulavirus.

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1093/nar/19.10.2739; PubMed=1645865 [NCBI, ExPASy, EBI, Israel, Japan]
Kawano M., Okamoto K., Bando H., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y.;
"Characterizations of the human parainfluenza type 2 virus gene encoding the L protein and the intergenic sequences.";
Nucleic Acids Res. 19:2739-2746(1991).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the paramyxoviruses L protein family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X57559; CAA40788.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S16664; S16664.

RefSeq

NP_598406.1; -.

3D structure databases

ModBase

P26676.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0019012;	Cellular component: virion (inferred from electronic annotation from UniProtKB-KW).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004482;	Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003723;	Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003968;	Molecular function: RNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0006370;	Biological process: mRNA capping (inferred from electronic annotation from UniProtKB-KW).
GO:0006410;	Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000830; RNA_pol_ParamyxoV; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P26676.

Genome annotation databases

GeneID

935189; -.

Other

DrugBank

DB00811; Ribavirin.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2262`	`2262`	`Large structural protein.`	`PRO_0000142734`
`DOMAIN`	`661 844`	`184`	`RdRp catalytic.`
`NP_BIND`	`1810 1819`	`10`	`ATP (Potential).`

Sequence information

Length: 2262 AA [This is the length of the unprocessed precursor]

Molecular weight: 256383 Da [This is the MW of the unprocessed precursor]

CRC64: 82468303F4453B48 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAASSEILLP EVHLNSPIVK HKLIYYLLLG HFPHDLDISE ISPLHNNDWD QIAREESNLA 

        70         80         90        100        110        120 
ERLGVAKSEL IKRVPAFRAT RWRSHAAVLI WPSCIPFLVK FLPHSKLQPV EQWYKLINAS 

       130        140        150        160        170        180 
CNTISDSIDR CMENISIKLT GKNNLFSRSR GTAGAGKNSK ITLNDIQSIW ESNKWQPNVS 

       190        200        210        220        230        240 
LWLTIKYQMR QLIMHQSSRQ PTDLVHIVDT RSGLIVITPE LVICFDRLNS VLMYFTFEMT 

       250        260        270        280        290        300 
LMVSDMFEGR MNVTALCTIS HYLSPLGPRI DRLFSIVDEL AQLLGDTVYK VIASLESLVY 

       310        320        330        340        350        360 
GCLQLKDPVV ELAGSFHSFI TQEIIDILIG SKALDKDESI TVTTQLLDIF SNLSPDLIAE 

       370        380        390        400        410        420 
MLCLMRLWGH PTLTAAQVGK VRESMCAGKL LDFPTIMKTL AFFHTILING YRRKKNGMWP 

       430        440        450        460        470        480 
PLILPKNASK SLIEFQHDNA EISYEYTLKH WKEISLIEFR KCFDFDPGEE LSIFMKDKAI 

       490        500        510        520        530        540 
SAPRSDWMSV FRRSLIKQRH QRHHIPMPNP FNRRLLLNFL EDDSFDPVAE LRYVTGGEYL 

       550        560        570        580        590        600 
QDDTFCASYS LKEKEIKPDG RIFAKLTNRM RSCQVIAEAI LANHAGTLMK ENGVVLNQLS 

       610        620        630        640        650        660 
LTKSLLTMSQ IGIISEKAKR YTRDNISSQG FHTIKTDSKN KRKSKTASSY LTDPDDTFEL 

       670        680        690        700        710        720 
SACFITTDLA KYCLQWRYQT IIHFARTLNR MYGVPHLFEW IHLRLIRSTL YVGDPFNPPA 

       730        740        750        760        770        780 
ATDAFDLDKV LNGDIFIVSK GGIEGLCQKM WTMISISVII LSSAESKTRV MSMVQGDNQA 

       790        800        810        820        830        840 
IAVTTRVPRS LPSIQKKELA YAASKLFFER LRANNYGLGH QLKAQETIIS STFFIYSKRV 

       850        860        870        880        890        900 
FYQGRILTQA LKNASKLCLT ADVLGECTQA SCSNSATTIM RLTENGVEKD TCYKLNIYQS 

       910        920        930        940        950        960 
IRQLTYDLIF PQYSIPGETI SEIFLQHPRL ISRIVLLPSQ LGGLNYLACS RLFNRNIGDP 

       970        980        990       1000       1010       1020 
LGTAVADLKR LIKCGALESW ILYNLLARKP GKGSWATLAA DPYSLNQEYL YPPTTILKRH 

      1030       1040       1050       1060       1070       1080 
TQNTLMEICR NPMLKGVFTD NAKEEENLLA KFLLDRDIVL PRVAHIIIDQ SSIGRKKQIQ 

      1090       1100       1110       1120       1130       1140 
GFFDTTRTIM RRSFEIKPLS TKKTLSVIEY NTNYLSYNYP VILNPLPIPG YLNYITDQTC 

      1150       1160       1170       1180       1190       1200 
SIDISRSLRK LSWSSLLNGR TLEGLETPDP IEVVNGFLIV GTGDCDFCMQ GDDKFTWFFL 

      1210       1220       1230       1240       1250       1260 
PMGIIIDGNP ETNPPIRVPY IGSRTEERRV ASMAYIKGAT HSLKAALRGA GVYIWAFGDT 

      1270       1280       1290       1300       1310       1320 
VVNWNDALDI ANTRVKISLE QLQTLTPLPT SANITHRLDD GATTLKFTPA SSYAFSSYTH 

      1330       1340       1350       1360       1370       1380 
ISNDQQYLEI DQRVVDSNII YQQLMITGLG IIETYHNPPI RTSTQEITLH LHTSSSCCVR 

      1390       1400       1410       1420       1430       1440 
SVDGCLICES NGEVPQITVP YTNTFVYDPD PLADYEIAHL DYLSYQAKIG STDYYSLTDK 

      1450       1460       1470       1480       1490       1500 
IDLLAHLTAK QMINSIIGLD ETVSIVNDAV ILSDYTNNWI SECSYTKIDL VFKLMAWNFL 

      1510       1520       1530       1540       1550       1560 
LELAFQMYYL RISSWTNIFD YTYMTLRRIP GTALNNIAAT ISHPKLLRRA MNLDIITPIH 

      1570       1580       1590       1600       1610       1620 
APYLASLDYV KLSIDAIQWG VKQVLADLSN GIDLEILILS EDSMEISDRA MNLIARKLTL 

      1630       1640       1650       1660       1670       1680 
LALVKGENYT FPKIKGMPPE EKCLVLTEYL AMCYQNTHHL DPDLQKYLYN LTNPKLTAFP 

      1690       1700       1710       1720       1730       1740 
SNNFYLTRKI LNQIRESDEG QYIITSYYES FEQLETDIIL HSTLTAPYDN SENSNKVRFI 

      1750       1760       1770       1780       1790       1800 
PFDIFPHPES LEKYPLPVDH DSQSAISTLI PGPPSHHVLR PLGVSSTAWY KGISYCRYLE 

      1810       1820       1830       1840       1850       1860 
TQKIQTGDHL YLAEGSGASM SLLELLFPGD TVYYNSLFSS GENPPQRNYA PLPTQFVQSV 

      1870       1880       1890       1900       1910       1920 
PYKLWQADLA DDSNLIKDFV PLWNGNGAVT DLSTKDAVAF IIHKVGAEKA SLVHIDLEST 

      1930       1940       1950       1960       1970       1980 
ANINQQTLSR SQIHSLIIAT TVLKRGGILI YKTSWLPFSR FSQLAGLLWC FFDRIHLIRS 

      1990       2000       2010       2020       2030       2040 
SYSDPHSHEV YLVCRLAADF RTIGFSAALV TATTLHNDGF TTIHPDVVCS YWQHHLENVG 

      2050       2060       2070       2080       2090       2100 
RVGKVIDEIL DGLATNFFAG DNGLILRCGG TPSSRKWLEI DQLASFDLVQ DALVTLITIH 

      2110       2120       2130       2140       2150       2160 
LKEIIEVQSS HTEDYTSLLF TPYNIGAAGK VRTIIKLILE RSLMYTVRNW LVLPSSIRDS 

      2170       2180       2190       2200       2210       2220 
VRQDLELGSF RLMSILSEQT FLKKTPTKKY LLDQLTRTYI STFFNSHSVL PLHRPYQKQI 

      2230       2240       2250       2260 
WKALGSVIYC SETVDIPLIK DIQIEDINDF EDIERGIDGE EL

P26676 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools