FUNCTION: Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which spontaneously decomposes to form allantoin.
CATALYTIC ACTIVITY: Urate + O₂ + H₂O = 5-hydroxyisourate + H₂O₂.
ENZYME REGULATION: Repressed by 20-hydroxyecdysone.
PATHWAY: Purine metabolism; uric acid degradation; (S)-allantoin from uric acid: step 1/3 .
SUBCELLULAR LOCATION: Peroxisome.
TISSUE SPECIFICITY: Malpighian tubules.
DEVELOPMENTAL STAGE: Third instar larvae and adult.
SIMILARITY: Belongs to the uricase family.

Copyright

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Cross-references

Sequence databases

EMBL

X57114; CAA40397.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S29133; S29133.

3D structure databases

HSSP

Q00511; 1UOX. [HSSP ENTRY / PDB]

ModBase

P23194.

Organism-specific databases

FlyBase

FBgn0013101; Dvir\Uro.

Ontologies

GO:0005777;	Cellular component: peroxisome (inferred from electronic annotation from UniProtKB-KW).
GO:0004846;	Molecular function: urate oxidase activity (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006144;	Biological process: purine base metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR002042; Uricase.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.270.10; Uricase; 1.

PANTHER

PTHR10395:SF1; Uricase; 1.

Pfam

PF01014; Uricase; 2.
Pfam graphical view of domain structure.

PRINTS

PR00093; URICASE.

ProDom

PD003367; Uricase; 2.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR03383; urate_oxi; 1.

PS00366; URICASE; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Oxidoreductase; Peroxisome; Purine metabolism.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 342`	`342`	`Uricase.`	`PRO_0000165994`
`MOTIF`	`340 342`	`3`	`Microbody targeting signal (Potential).`
`ACT_SITE`	`221 221`		`Charge relay system (By similarity).`
`ACT_SITE`	`270 270`		`Charge relay system (By similarity).`

Sequence information

Length: 342 AA [This is the length of the unprocessed precursor]

Molecular weight: 38724 Da [This is the MW of the unprocessed precursor]

CRC64: 4DE10D5485294723 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MFATPLRQLS SLKRSGIAGQ EQAQLYEITN KGYGKDAVKV MHINRKGPVH SIQELEVGTH 

        70         80         90        100        110        120 
LKLYSNKDYM LGNNSDVVAT DSQKNTVYLL AKKHGIESPE KFALILAKHF LSTYAHVEEV 

       130        140        150        160        170        180 
HVHVEAYPWQ RMTQDVSDNI GKGYCENNCN SRSNGNCQLH NHAFIFTPTA HDYCDVILTR 

       190        200        210        220        230        240 
QDPKQTVISG IKGLRVLKTT QSSFVNFVDD EFRTLADQYD RIFSTVVECS WEYSDTESVN 

       250        260        270        280        290        300 
FLHAWETVKD IVVRNFAGDP SVGIPSPSVQ HTLYLSEKQV LDVLPQVSVV SMTMPNKHYF 

       310        320        330        340 
NFDTKPFQQL VPGENNEVFI PTDKPHGTIY AQLSRKSLKS HL

P23194 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools