ID LUXB2_PHOLU Reviewed; 324 AA. AC P23147; DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1991, sequence version 1. DT 25-NOV-2008, entry version 45. DE RecName: Full=Alkanal monooxygenase beta chain; DE EC=1.14.14.3; DE AltName: Full=Bacterial luciferase beta chain; GN Name=luxB; OS Photorhabdus luminescens (Xenorhabdus luminescens). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Photorhabdus. OX NCBI_TaxID=29488; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=Hw; RX MEDLINE=91139581; PubMed=1995589; RA Xi L., Cho K.W., Tu S.C.; RT "Cloning and nucleotide sequences of lux genes and characterization of RT luciferase of Xenorhabdus luminescens from a human wound."; RL J. Bacteriol. 173:1399-1405(1991). CC -!- FUNCTION: Light-emitting reaction in luminous bacteria. The CC specific role of the beta subunit is unknown, but it is absolutely CC required for bioluminescence activity. CC -!- CATALYTIC ACTIVITY: RCHO + reduced FMN + O(2) = RCOOH + FMN + CC H(2)O + light. CC -!- SUBUNIT: Heterodimer of an alpha and a beta chain. CC -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase CC family. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; M62917; AAA63566.1; -; Genomic_DNA. DR HSSP; P07739; 1BSL. DR GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:EC. DR GO; GO:0008218; P:bioluminescence; IEA:InterPro. DR GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW. DR InterPro; IPR011251; Luciferase-like_bac. DR InterPro; IPR002103; Luciferase_bac. DR InterPro; IPR016048; Luciferase_mOase. DR Gene3D; G3DSA:3.20.20.30; Luciferase_like; 1. DR Pfam; PF00296; Bac_luciferase; 1. DR PRINTS; PR00089; LUCIFERASE. DR PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1. PE 3: Inferred from homology; KW Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase; KW Photoprotein. FT CHAIN 1 324 Alkanal monooxygenase beta chain. FT /FTId=PRO_0000220178. SQ SEQUENCE 324 AA; 37115 MW; E7D81324D344A975 CRC64; MKFGLFFLNF INSTTIQEQS IARMQEITEY VDKLNFEQIL VCENHFSDNG VVGAPLTVSG FLLGLTEKIK IGSLNHVITT HHPVRIAEEA CLLDQLSEGR FILGFSDCER KDEMPFFNRP EQYQQQLFEE CYDIINDALT TGYCNPNGDF YNFPKISMNP HAYTQNGPRK YVTATSCHVV EWAAKKGIPL IFKWDDSNEV KHEYAKRYQA IAGEYGVDLA EIDHQLMILV NYSEDSEKAK EETRAFISDY ILAMHPNENF EKKLEEIITE NSVGDYMECT TAAKLAMEKC GTKGILLSFE SMSDFTHQIN AIDIVNDNIK KYHM //