ID   LDH2_LACLC              Reviewed;         314 AA.
AC   P0C2T6; Q7WYP6;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 1.
DT   25-NOV-2008, entry version 13.
DE   RecName: Full=L-lactate dehydrogenase 2;
DE            Short=L-LDH 2;
DE            EC=1.1.1.27;
GN   Name=ldhB;
OS   Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OC   Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Lactococcus.
OX   NCBI_TaxID=1359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Gaspar P., Coelho P., Neves A.R., Shearman C.A., Gasson M.J.,
RA   Soares C.M., Baptisa A.M., Ramos A., Santos H.;
RT   "Characterization of a lactate dehydrogenase encoded by the ldhB gene
RT   of Lactococcus lactis.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-
CC       lactate from pyruvate: step 1/1.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
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DR   EMBL; AY236961; AAP49572.1; -; Genomic_DNA.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0019642; P:anaerobic glycolysis; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00488; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DHase.
DR   InterPro; IPR011304; L-lactate_DHase.
DR   InterPro; IPR001236; Lactate/malate_DHase.
DR   InterPro; IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR   PROSITE; PS00064; L_LDH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase; Phosphoprotein.
FT   CHAIN         1    314       L-lactate dehydrogenase 2.
FT                                /FTId=PRO_0000168355.
FT   NP_BIND      14     42       NAD (By similarity).
FT   ACT_SITE    178    178       Proton acceptor (By similarity).
FT   BINDING      91     91       Substrate (By similarity).
FT   BINDING     123    123       NAD or substrate (By similarity).
FT   BINDING     154    154       Substrate (By similarity).
FT   BINDING     232    232       Substrate (By similarity).
FT   MOD_RES     223    223       Phosphotyrosine (By similarity).
SQ   SEQUENCE   314 AA;  34398 MW;  9D5114712ACB75A4 CRC64;
     MKITSRKVVV IGTGFVGTSI AYSMINQGLV NELVLIDVNQ DKAEGEALDL LDGISWAQEN
     VIVRAGNYKD CENADIVVIT AGVNQKPGQS RLDLVNTNAK IMRSIVTQVM DSGFDGIFVI
     ASNPVDILTY VAWETSGLDQ SRIVGTGTTL DTTRFRKELA TKLEIDPRSV HGYIIGEHGD
     SEVAVWSHTT IGGKPILEFI VKNKKIGLED LSNLSNKVKN AAYEIIDKKQ ATYYGIGMST
     ARIVKAILNN EQVILPVSAY LRGEYGQEGV FTGVPSVVNQ NGVREIIELN IDAYEMKQFE
     KSVSQLKEVI ESIK
//