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UniProtKB/Swiss-Prot entry P0AE52


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name BCP_ECOLI
Primary accession number P0AE52
Secondary accession number P23480
Integrated into Swiss-Prot on December 6, 2005
Sequence was last modified on December 6, 2005 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 26)
Name and origin of the protein
Protein name Putative peroxiredoxin bcp
Synonyms EC 1.11.1.15
Thioredoxin reductase
Bacterioferritin comigratory protein
Gene name
Name: bcp
OrderedLocusNames: b2480, JW2465
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
PubMed=2016588 [NCBI, ExPASy, EBI, Israel, Japan]
Andrews S.C., Harrison P.M., Guest J.R.;
"A molecular analysis of the 53.3 minute region of the Escherichia coli linkage map.";
J. Gen. Microbiol. 137:361-367(1991).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/4.2.91; PubMed=9205837 [NCBI, ExPASy, EBI, Israel, Japan]
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.";
DNA Res. 4:91-113(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
STRAIN=K12;
PubMed=9537378 [NCBI, ExPASy, EBI, Israel, Japan]
Ghrist A.C., Stauffer G.V.;
"Promoter characterization and constitutive expression of the Escherichia coli gcvR gene.";
J. Bacteriol. 180:1803-1807(1998).
[6]
PROTEIN SEQUENCE OF 1-15.
STRAIN=K12 / EMG2;
DOI=10.1002/elps.1150180807; PubMed=9298646 [NCBI, ExPASy, EBI, Israel, Japan]
Link A.J., Robison K., Church G.M.;
"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.";
Electrophoresis 18:1259-1313(1997).
[7]
PROTEIN SEQUENCE OF 1-11.
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
Frutiger S., Hughes G.J., Pasquali C., Hochstrasser D.F.;
Submitted (FEB-1996) to UniProtKB.
[8]
ACTIVE SITE CYS-46.
DOI=10.1074/jbc.275.4.2924; PubMed=10644761 [NCBI, ExPASy, EBI, Israel, Japan]
Jeong W., Cha M.K., Kim I.H.;
"Thioredoxin-dependent hydroperoxide peroxidase activity of bacterioferritin comigratory protein (BCP) as a new member of the thiol-specific antioxidant protein (TSA)/Alkyl hydroperoxide peroxidase C (AhpC) family.";
J. Biol. Chem. 275:2924-2930(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M63654; AAB88562.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC75533.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA16358.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF023337; AAC46233.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B49749; B49749.
RefSeq AP_003066.1; -.
NP_416975.1; -.
3D structure databases
ModBase P0AE52.
Enzyme and pathway databases
BioCyc EcoCyc:EG10108-MON; -.
MetaCyc:EG10108-MON; -.
2D gel databases
SWISS-2DPAGE P0AE52; -.
Organism-specific databases
EchoBASE EB0106; -.
EcoGene EG10108; bcp.
Ontologies
GO
GO:0051920; Molecular function: peroxiredoxin activity (inferred from electronic annotation from EC).
GO:0045454; Biological process: cell redox homeostasis (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR000866; AhpC-TSA.
IPR012335; Thioredoxin_fold.
Graphical view of domain structure.
Gene3D G3DSA:3.40.30.10; Thioredoxin_fold; 1.
Pfam PF00578; AhpC-TSA; 1.
Pfam graphical view of domain structure.
PROSITE PS51352; THIOREDOXIN_2; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
GeneID 946949; -.
GenomeReviews AP009048_GR; JW2465.
U00096_GR; b2480.
KEGG ecj:JW2465; -.
eco:b2480; -.
Phylogenomic databases
HOGENOM P0AE52; -.
Genome annotation databases
CMR P0AE52; b2480.
Other
ProtoNet P0AE52.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; Oxidoreductase; Peroxidase; Redox-active center.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom  To Length Description FTId
CHAIN   1   156  156     Putative peroxiredoxin bcp. PRO_0000135134
DOMAIN   4   156  153     Thioredoxin. 
ACT_SITE   46    46        Cysteine sulfenic acid (-SOH) intermediate. 
Sequence information
Length: 156 AA [This is the length of the unprocessed precursor] Molecular weight: 17634 Da [This is the MW of the unprocessed precursor] CRC64: C7D267A671409EFC [This is a checksum on the sequence]
        10         20         30         40         50         60 
MNPLKAGDIA PKFSLPDQDG EQVNLTDFQG QRVLVYFYPK AMTPGCTVQA CGLRDNMDEL 

        70         80         90        100        110        120 
KKAGVDVLGI STDKPEKLSR FAEKELLNFT LLSDEDHQVC EQFGVWGEKS FMGKTYDGIH 

       130        140        150 
RISFLIDADG KIEHVFDDFK TSNHHDVVLN WLKEHA 

P0AE52 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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