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UniProtKB/Swiss-Prot entry P0ACE1


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MBHM_ECO57
Primary accession number P0ACE1
Secondary accession number P37181
Integrated into Swiss-Prot on November 22, 2005
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    December 16, 2008 (Entry version 25)
Name and origin of the protein
Protein name Hydrogenase-2 large chain [Precursor]
Synonyms HYD2
EC 1.12.99.6
Membrane-bound hydrogenase 2 large subunit
NiFe hydrogenase
Gene name
Name: hybC
OrderedLocusNames: Z4348, ECs3879
From
Escherichia coli O157:H7 [TaxID: 83334] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
DOI=10.1038/35054089; PubMed=11206551 [NCBI, ExPASy, EBI, Israel, Japan]
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.;
"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
Nature 409:529-533(2001).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
DOI=10.1093/dnares/8.1.11; PubMed=11258796 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S., Shiba T., Hattori M., Shinagawa H.;
"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12.";
DNA Res. 8:11-22(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE005174; AAG58131.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000007; BAB37302.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR G85958; G85958.
G91113; G91113.
RefSeq NP_289572.1; -.
NP_311906.1; -.
3D structure databases
HSSP P21852; 1UBL. [HSSP ENTRY / PDB]
ModBase P0ACE1.
Enzyme and pathway databases
BioCyc ECOL83334:ECS3879-MON; -.
Ontologies
GO
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0008901; Molecular function: ferredoxin hydrogenase activity (inferred from electronic annotation from InterPro).
GO:0033748; Molecular function: hydrogenase (acceptor) activity (inferred from electronic annotation from EC).
GO:0016151; Molecular function: nickel ion binding (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.
Pfam PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.
PROSITE PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.
Genome annotation databases
GeneID 916295; -.
958476; -.
GenomeReviews AE005174_GR; Z4348.
BA000007_GR; ECs3879.
KEGG ece:Z4348; -.
ecs:ECs3879; -.
Phylogenomic databases
HOGENOM P0ACE1; -.
Genome annotation databases
CMR P0ACE1; Z4348.
Other
ProtoNet P0ACE1.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell membrane; Complete proteome; Membrane; Metal-binding; Nickel; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed (By similarity). 
CHAIN   2   552  551     Hydrogenase-2 large chain. PRO_0000042990
PROPEP   553   567  15      PRO_0000042991
METAL   61    61        Nickel (Potential). 
METAL   64    64        Nickel (Potential). 
METAL   546   546        Nickel (Potential). 
METAL   549   549        Nickel (Potential). 
SITE   552   553  2     Cleavage; by hybD (By similarity). 
Sequence information
Length: 567 AA [This is the length of the unprocessed precursor] Molecular weight: 62491 Da [This is the MW of the unprocessed precursor] CRC64: A19E6F513390F29E [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSQRITIDPV TRIEGHLRID CEIENGVVSK AWASGTMWRG MEEIVKNRDP RDAWMIVQRI 

        70         80         90        100        110        120 
CGVCTTTHAL SSVRAAESAL NIDVPVNAQY IRNIILAAHT THDHIVHFYQ LSALDWVDIT 

       130        140        150        160        170        180 
SALQADPTKA SEMLKGVSTW HLNSPEEFTK VQNKIKDLVA SGQLGIFANG YWGHPAMKLP 

       190        200        210        220        230        240 
PEVNLIAVAH YLQALECQRD ANRVVALLGG KTPHIQNLAV GGVANPINLD GLGVLNLERL 

       250        260        270        280        290        300 
MYIKSFIDKL SDFVEQVYKV DTAVIAAFYP EWLTRGKGAV NYLSVPEFPT DSKNGSFLFP 

       310        320        330        340        350        360 
GGYIENADLS SYRPITSHSD EYLIKGIQES AKHSWYKDEA PQAPWEGTTI PAYDGWSDDG 

       370        380        390        400        410        420 
KYSWVKSPTF YGKTVEVGPL ANMLVKLAAG RESTQNKLNE IVAIYQKLTG NTLEVAQLHS 

       430        440        450        460        470        480 
TLGRIIGRTV HCCELQDILQ NQYSALITNI GKGDHTTFVK PNIPATGEFK GVGFLEAPRG 

       490        500        510        520        530        540 
MLSHWMVIKD GIISNYQAVV PSTWNSGPRN FNDDVGPYEQ SLVGTPVADP NKPLEVVRTI 

       550        560 
HSFDPCMACA VHVVDADGNE VVSVKVL 

P0ACE1 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
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