ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P0ACE0


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name MBHM_ECOLI
Primary accession number P0ACE0
Secondary accession numbers P37181 Q2M9K1
Integrated into Swiss-Prot on November 22, 2005
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    December 16, 2008 (Entry version 29)
Name and origin of the protein
Protein name Hydrogenase-2 large chain [Precursor]
Synonyms HYD2
EC 1.12.99.6
Membrane-bound hydrogenase 2 large subunit
NiFe hydrogenase
Gene name
Name: hybC
OrderedLocusNames: b2994, JW2962
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12 / TG1;
PubMed=8021226 [NCBI, ExPASy, EBI, Israel, Japan]
Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T., Peck H.D. Jr., Przybyla A.E.;
"Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.";
J. Bacteriol. 176:4416-4423(1994).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[4]
PROTEIN SEQUENCE OF 2-7.
PubMed=9738917 [NCBI, ExPASy, EBI, Israel, Japan]
Sargent F., Ballantine S.P., Rugman P.A., Palmer T., Boxer D.H.;
"Reassignment of the gene encoding the Escherichia coli hydrogenase 2 small subunit -- identification of a soluble precursor of the small subunit in a hypB mutant.";
Eur. J. Biochem. 255:746-754(1998).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U09177; AAA21591.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U28377; AAA69161.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC76030.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE77055.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR C55516; C55516.
RefSeq AP_003549.1; -.
NP_417468.1; -.
3D structure databases
HSSP P21852; 1UBL. [HSSP ENTRY / PDB]
ModBase P0ACE0.
Enzyme and pathway databases
BioCyc EcoCyc:HYBC-MON; -.
MetaCyc:HYBC-MON; -.
Organism-specific databases
EchoBASE EB1749; -.
EcoGene EG11801; hybC.
Ontologies
GO
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0008901; Molecular function: ferredoxin hydrogenase activity (inferred from electronic annotation from InterPro).
GO:0033748; Molecular function: hydrogenase (acceptor) activity (inferred from electronic annotation from EC).
GO:0016151; Molecular function: nickel ion binding (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.
Pfam PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.
PROSITE PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.
Genome annotation databases
GeneID 945182; -.
GenomeReviews AP009048_GR; JW2962.
U00096_GR; b2994.
KEGG ecj:JW2962; -.
eco:b2994; -.
Phylogenomic databases
HOGENOM P0ACE0; -.
Genome annotation databases
CMR P0ACE0; b2994.
Other
ProtoNet P0ACE0.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell membrane; Complete proteome; Direct protein sequencing; Membrane; Metal-binding; Nickel; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   552  551     Hydrogenase-2 large chain. PRO_0000013407
PROPEP   553   567  15      PRO_0000013408
METAL   61    61        Nickel (Potential). 
METAL   64    64        Nickel (Potential). 
METAL   546   546        Nickel (Potential). 
METAL   549   549        Nickel (Potential). 
SITE   552   553  2     Cleavage; by hybD. 
Sequence information
Length: 567 AA [This is the length of the unprocessed precursor] Molecular weight: 62491 Da [This is the MW of the unprocessed precursor] CRC64: A19E6F513390F29E [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSQRITIDPV TRIEGHLRID CEIENGVVSK AWASGTMWRG MEEIVKNRDP RDAWMIVQRI 

        70         80         90        100        110        120 
CGVCTTTHAL SSVRAAESAL NIDVPVNAQY IRNIILAAHT THDHIVHFYQ LSALDWVDIT 

       130        140        150        160        170        180 
SALQADPTKA SEMLKGVSTW HLNSPEEFTK VQNKIKDLVA SGQLGIFANG YWGHPAMKLP 

       190        200        210        220        230        240 
PEVNLIAVAH YLQALECQRD ANRVVALLGG KTPHIQNLAV GGVANPINLD GLGVLNLERL 

       250        260        270        280        290        300 
MYIKSFIDKL SDFVEQVYKV DTAVIAAFYP EWLTRGKGAV NYLSVPEFPT DSKNGSFLFP 

       310        320        330        340        350        360 
GGYIENADLS SYRPITSHSD EYLIKGIQES AKHSWYKDEA PQAPWEGTTI PAYDGWSDDG 

       370        380        390        400        410        420 
KYSWVKSPTF YGKTVEVGPL ANMLVKLAAG RESTQNKLNE IVAIYQKLTG NTLEVAQLHS 

       430        440        450        460        470        480 
TLGRIIGRTV HCCELQDILQ NQYSALITNI GKGDHTTFVK PNIPATGEFK GVGFLEAPRG 

       490        500        510        520        530        540 
MLSHWMVIKD GIISNYQAVV PSTWNSGPRN FNDDVGPYEQ SLVGTPVADP NKPLEVVRTI 

       550        560 
HSFDPCMACA VHVVDADGNE VVSVKVL 

P0ACE0 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by au flag APAF Australia Mirror sites: Brazil  Canada  China  Korea  Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!