NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(94)00905-8; PubMed=7867952 [NCBI, ExPASy, EBI, Israel, Japan]
Sundstroem L., Jansson C., Bremer K., Heikkila E., Olsson-Liljequist B., Skoeld O.;
"A new dhfrVIII trimethoprim-resistance gene, flanked by IS26, whose product is remote from other dihydrofolate reductases in parsimony analysis.";
Gene 154:7-14(1995).

Comments

FUNCTION: Confers high-level trimethoprim resistance.
CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH.
PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1 .
SUBUNIT: Homodimer (By similarity).
MISCELLANEOUS: The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
SIMILARITY: Belongs to the dihydrofolate reductase family.
SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U10186; AAA68493.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

I60308; I60308.

RefSeq

YP_001569075.1; -.

3D structure databases

HSSP

P00381; 3DFR. [HSSP ENTRY / PDB]

ModBase

P0ABQ7.

Ontologies

GO:0004146;	Molecular function: dihydrofolate reductase activity (inferred from electronic annotation from InterPro).
GO:0050661;	Molecular function: NADP binding (inferred from electronic annotation from InterPro).
GO:0006545;	Biological process: glycine biosynthetic process (inferred from electronic annotation from InterPro).
GO:0009165;	Biological process: nucleotide biosynthetic process (inferred from electronic annotation from InterPro).
GO:0006730;	Biological process: one-carbon compound metabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0046677;	Biological process: response to antibiotic (inferred from electronic annotation from UniProtKB-KW).
GO:0031427;	Biological process: response to methotrexate (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR012259; DHFR.
IPR001796; DHFR_reg.
Graphical view of domain structure.

PANTHER

PTHR11549:SF1; DHFR; 1.

Pfam

PF00186; DHFR_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00070; DHFR.

PROSITE

PS00075; DHFR_1; 1.
PS51330; DHFR_2; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

GeneID

5759757; -.

Other

ProtoNet

P0ABQ7.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Antibiotic resistance; Methotrexate resistance; NADP; One-carbon metabolism; Oxidoreductase; Plasmid; Trimethoprim resistance.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 169`	`169`	`Dihydrofolate reductase type 8.`	`PRO_0000186425`
`DOMAIN`	`3 169`	`167`	`DHFR.`

Sequence information

Length: 169 AA [This is the length of the unprocessed precursor]

Molecular weight: 19021 Da [This is the MW of the unprocessed precursor]

CRC64: 76E1ABA0C0DE30CC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MIELHAILAA TANGCIGKDN ALPWPPLKGD LARFKKLTMG KVVIMGRKTY ESLPVKLEGR 

        70         80         90        100        110        120 
TCIVMTRQAL ELPGVRDANG AIFVNNVSDA MRFAQEESVG DVAYVIGGAE IFKRLALMIT 

       130        140        150        160 
QIELTFVKRL YEGDTYVDLA EMVKDYEQNG MEEHDLHTYF TYRKKELTE

P0ABQ7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools