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UniProtKB/Swiss-Prot entry P0AB67


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PNTB_ECOLI
Primary accession number P0AB67
Secondary accession numbers P07002 P76890
Integrated into Swiss-Prot on April 1, 1988
Sequence was last modified on November 8, 2005 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 34)
Name and origin of the protein
Protein name NAD(P) transhydrogenase subunit beta
Synonyms EC 1.6.1.2
Nicotinamide nucleotide transhydrogenase subunit beta
Pyridine nucleotide transhydrogenase subunit beta
Gene name
Name: pntB
OrderedLocusNames: b1602, JW1594
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3525165 [NCBI, ExPASy, EBI, Israel, Japan]
Clarke D.M., Loo T.W., Gillam S., Bragg P.D.;
"Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli.";
Eur. J. Biochem. 158:647-653(1986).
[2]
SEQUENCE REVISION.
PubMed=1633824 [NCBI, ExPASy, EBI, Israel, Japan]
Ahmad S., Glavas N.A., Bragg P.D.;
"A mutation at Gly314 of the beta subunit of the Escherichia coli pyridine nucleotide transhydrogenase abolishes activity and affects the NADP(H)-induced conformational change.";
Eur. J. Biochem. 207:733-739(1992).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.6.363; PubMed=9097039 [NCBI, ExPASy, EBI, Israel, Japan]
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.";
DNA Res. 3:363-377(1996).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[6]
PROTEIN SEQUENCE OF 266-277 AND 364-373.
DOI=10.1016/0167-4838(91)90106-A; PubMed=1932078 [NCBI, ExPASy, EBI, Israel, Japan]
Tong R.C., Glavas N.A., Bragg P.D.;
"Topological analysis of the pyridine nucleotide transhydrogenase of Escherichia coli using proteolytic enzymes.";
Biochim. Biophys. Acta 1080:19-28(1991).
[7]
TOPOLOGY [LARGE SCALE ANALYSIS].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.1109730; PubMed=15919996 [NCBI, ExPASy, EBI, Israel, Japan]
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
"Global topology analysis of the Escherichia coli inner membrane proteome.";
Science 308:1321-1323(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X04195; CAB37090.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X66086; CAA46885.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC74674.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA15336.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S24381; DEECXB.
RefSeq AP_002223.1; -.
NP_416119.1; -.
3D structure databases
PDB
2BRU; NMR; -; C=286-462.[ExPASy / RCSB / EBI]
PDBsum 2BRU; -.
ModBase P0AB67.
Enzyme and pathway databases
BioCyc EcoCyc:PNTB-MON; -.
MetaCyc:PNTB-MON; -.
Organism-specific databases
EchoBASE EB0738; -.
EcoGene EG10745; pntB.
Ontologies
GO
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0008750; Molecular function: NAD(P)+ transhydrogenase (AB-specific) activity (inferred from electronic annotation from InterPro).
GO:0050661; Molecular function: NADP binding (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR012136; NADH_DH_b.
IPR004003; NADP_transhyd_b.
Graphical view of domain structure.
Pfam PF02233; PNTB; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000204; PNTB; 1.
Genome annotation databases
GeneID 946144; -.
GenomeReviews AP009048_GR; JW1594.
U00096_GR; b1602.
KEGG ecj:JW1594; -.
eco:b1602; -.
Phylogenomic databases
HOGENOM P0AB67; -.
Genome annotation databases
CMR P0AB67; b1602.
Other
ProtoNet P0AB67.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Cell inner membrane; Cell membrane; Complete proteome; Direct protein sequencing; Membrane; NAD; NADP; Oxidoreductase; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   462  462     NAD(P) transhydrogenase subunit beta. PRO_0000199023
TOPO_DOM   1     3  3     Periplasmic (Potential). 
TRANSMEM   4    24  21     Potential. 
TOPO_DOM   25    45  21     Cytoplasmic (Potential). 
TRANSMEM   46    66  21     Potential. 
TOPO_DOM   67    82  16     Periplasmic (Potential). 
TRANSMEM   83   103  21     Potential. 
TOPO_DOM   104   115  12     Cytoplasmic (Potential). 
TRANSMEM   116   136  21     Potential. 
TOPO_DOM   137   164  28     Periplasmic (Potential). 
TRANSMEM   165   185  21     Potential. 
TOPO_DOM   186   188  3     Cytoplasmic (Potential). 
TRANSMEM   189   209  21     Potential. 
TOPO_DOM   210   215  6     Periplasmic (Potential). 
TRANSMEM   216   236  21     Potential. 
TOPO_DOM   237   239  3     Cytoplasmic (Potential). 
TRANSMEM   240   260  21     Potential. 
TOPO_DOM   261   308  48     Periplasmic (Potential). 
TRANSMEM   309   329  21     Potential. 
TOPO_DOM   330   462  133     Cytoplasmic (Potential). 
HELIX   297   305  9      
STRAND   307   312  6      
HELIX   316   319  4      
TURN   320   322  3      
HELIX   323   336  14      
STRAND   339   344  6      
STRAND   346   353  8      
HELIX   355   362  8      
TURN   366   368  3      
STRAND   369   372  4      
HELIX   376   381  6      
STRAND   383   387  5      
HELIX   391   394  4      
HELIX   396   398  3      
STRAND   404   406  3      
STRAND   417   423  7      
STRAND   425   427  3      
TURN   437   439  3      
STRAND   440   447  8      
HELIX   451   461  11      
Sequence information
Length: 462 AA [This is the length of the unprocessed precursor] Molecular weight: 48723 Da [This is the MW of the unprocessed precursor] CRC64: D11747291D271A2F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSGGLVTAAY IVAAILFIFS LAGLSKHETS RQGNNFGIAG MAIALIATIF GPDTGNVGWI 

        70         80         90        100        110        120 
LLAMVIGGAI GIRLAKKVEM TEMPELVAIL HSFVGLAAVL VGFNSYLHHD AGMAPILVNI 

       130        140        150        160        170        180 
HLTEVFLGIF IGAVTFTGSV VAFGKLCGKI SSKPLMLPNR HKMNLAALVV SFLLLIVFVR 

       190        200        210        220        230        240 
TDSVGLQVLA LLIMTAIALV FGWHLVASIG GADMPVVVSM LNSYSGWAAA AAGFMLSNDL 

       250        260        270        280        290        300 
LIVTGALVGS SGAILSYIMC KAMNRSFISV IAGGFGTDGS STGDDQEVGE HREITAEETA 

       310        320        330        340        350        360 
ELLKNSHSVI ITPGYGMAVA QAQYPVAEIT EKLRARGINV RFGIHPVAGR LPGHMNVLLA 

       370        380        390        400        410        420 
EAKVPYDIVL EMDEINDDFA DTDTVLVIGA NDTVNPAAQD DPKSPIAGMP VLEVWKAQNV 

       430        440        450        460 
IVFKRSMNTG YAGVQNPLFF KENTHMLFGD AKASVDAILK AL 

P0AB67 in FASTA format

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