[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE. DOI=10.1093/nar/10.23.7701; PubMed=6296787 [NCBI, ExPASy, EBI, Israel, Japan] Deutch A.H., Smith C.J., Rushlow K.E., Kretschmer P.J.; "Escherichia coli delta 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification."; Nucleic Acids Res. 10:7701-7714(1982).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / MG1655 / ATCC 47076; Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.; "Sequence of minutes 4-25 of Escherichia coli."; Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / MG1655 / ATCC 47076; DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan] Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.; "The complete genome sequence of Escherichia coli K-12."; Science 277:1453-1474(1997).
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan] Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."; Mol. Syst. Biol. 2:E1-E5(2006).

Comments

CATALYTIC ACTIVITY: L-proline + NAD(P)⁺ = 1-pyrroline-5-carboxylate + NAD(P)H.
PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1 .
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the pyrroline-5-carboxylate reductase family.

Copyright

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Cross-references

Sequence databases

EMBL

J01665; AAA86433.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U73857; AAB18110.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC73489.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE76167.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A00385; RDECC.

RefSeq

AP_001037.1; -.
NP_414920.1; -.

3D structure databases

ModBase

P0A9L8.

Enzyme and pathway databases

BioCyc

EcoCyc:PYRROLINECARBREDUCT-MON; -.
MetaCyc:PYRROLINECARBREDUCT-MON; -.

Organism-specific databases

EchoBASE

EB0762; -.

EcoGene

EG10769; proC.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0005488;	Molecular function: binding (inferred from electronic annotation from InterPro).
GO:0004735;	Molecular function: pyrroline-5-carboxylate reductase activity (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006561;	Biological process: proline biosynthetic process (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR016040; NAD(P)-bd.
IPR004455; NADP_OxRdtase_F420.
IPR000304; P5CR.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR11645; P5CR; 1.

Pfam

PF03807; F420_oxidored; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000193; Pyrrol-5-carb_rd; 1.

TIGRFAMs

TIGR00112; proC; 1.

PROSITE

PS00521; P5CR; 1.

Genome annotation databases

GeneID

945034; -.

GenomeReviews

AP009048_GR; JW0377.
U00096_GR; b0386.

KEGG

ecj:JW0377; -.
eco:b0386; -.

Phylogenomic databases

HOGENOM

P0A9L8; -.

Other

LinkHub

P0A9L8; -.

Genome annotation databases

CMR

P0A9L8; b0386.

Other

ProtoNet

P0A9L8.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Amino-acid biosynthesis; Complete proteome; Cytoplasm; Direct protein sequencing; NADP; Oxidoreductase; Proline biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 269`	`269`		`Pyrroline-5-carboxylate reductase.`	`PRO_0000187289`

Sequence information

Length: 269 AA [This is the length of the unprocessed precursor]

Molecular weight: 28145 Da [This is the MW of the unprocessed precursor]

CRC64: C5E2CED36BCC508D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEKKIGFIGC GNMGKAILGG LIASGQVLPG QIWVYTPSPD KVAALHDQFG INAAESAQEV 

        70         80         90        100        110        120 
AQIADIIFAA VKPGIMIKVL SEITSSLNKD SLVVSIAAGV TLDQLARALG HDRKIIRAMP 

       130        140        150        160        170        180 
NTPALVNAGM TSVTPNALVT PEDTADVLNI FRCFGEAEVI AEPMIHPVVG VSGSSPAYVF 

       190        200        210        220        230        240 
MFIEAMADAA VLGGMPRAQA YKFAAQAVMG SAKMVLETGE HPGALKDMVC SPGGTTIEAV 

       250        260 
RVLEEKGFRA AVIEAMTKCM EKSEKLSKS

P0A9L8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools