[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=K12; DOI=10.1093/nar/16.22.10803; PubMed=2849753 [NCBI, ExPASy, EBI, Israel, Japan] Sands J.F., Regnier P., Cummings H.S., Grunberg-Manago M., Hershey J.W.B.; "The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping."; Nucleic Acids Res. 16:10803-10816(1988).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / MG1655 / ATCC 47076; DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan] Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.; "The complete genome sequence of Escherichia coli K-12."; Science 277:1453-1474(1997).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan] Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."; Mol. Syst. Biol. 2:E1-E5(2006).
[4]	PARTIAL PROTEIN SEQUENCE OF 2-12. STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; Frutiger S., Hughes G.J., Tonella L., Hochstrasser D.F.; Submitted (SEP-1996) to UniProtKB.
[5]	IDENTIFICATION. PubMed=7535280 [NCBI, ExPASy, EBI, Israel, Japan] Dammel C.S., Noller H.F.; "Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA."; Genes Dev. 9:626-637(1995).
[6]	FUNCTION. STRAIN=MW100; PubMed=9422595 [NCBI, ExPASy, EBI, Israel, Japan] Bylund G.O., Wipemo L.C., Lundberg L.A., Wikstroem P.M.; "RimM and RbfA are essential for efficient processing of 16S rRNA in Escherichia coli."; J. Bacteriol. 180:73-82(1998).

Comments

FUNCTION: Essential for efficient processing of 16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. Could be some accessory protein needed for efficient assembly of the 30S subunit. May interact with the 5'-terminal helix region of 16S rRNA. Has affinity for free ribosomal 30S subunits but not for 70S ribosomes.
FUNCTION: Overexpression suppresses rimM deletion mutants.
INTERACTION:
P18843:nadE; NbExp=1; IntAct=EBI-1131774, EBI-548960;
SUBCELLULAR LOCATION: Cytoplasm (Potential).
SIMILARITY: Belongs to the rbfA family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X13270; CAA31634.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X13775; CAA32020.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U18997; AAA57970.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC76201.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE77213.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S01915; Q9EC15.

RefSeq

AP_003712.1; -.
NP_417636.1; -.

3D structure databases

PDB

1KKG; NMR; -; A=1-108.

[ExPASy / RCSB / EBI]

PDBsum

1KKG; -.

ModBase

P0A7G2.

Protein-protein interaction databases

IntAct

P0A7G2; 2.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11178-MON; -.

2D gel databases

SWISS-2DPAGE

P0A7G2; -.

Organism-specific databases

EchoBASE

EB1165; -.

EcoGene

EG11178; rbfA.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0005515;	Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006364;	Biological process: rRNA processing (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00003; -; 1.
PBIL [Tree]

InterPro

IPR000238; Rib_bd_factA.
Graphical view of domain structure.

Pfam

PF02033; RBFA; 1.
Pfam graphical view of domain structure.

ProDom

PD007327; Rib_bind_factA; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR00082; rbfA; 1.

PROSITE

PS01319; RBFA; 1.

Genome annotation databases

GeneID

947685; -.

GenomeReviews

AP009048_GR; JW3136.
U00096_GR; b3167.

KEGG

ecj:JW3136; -.
eco:b3167; -.

Phylogenomic databases

HOGENOM

P0A7G2; -.

Other

LinkHub

P0A7G2; -.

Genome annotation databases

CMR

P0A7G2; b3167.

Other

ProtoNet

P0A7G2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; rRNA processing.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 133`	`132`	`Ribosome-binding factor A.`	`PRO_0000102658`
`STRAND`	`5 7`	`3`
`HELIX`	`8 22`	`15`
`STRAND`	`25 29`	`5`
`HELIX`	`30 32`	`3`
`STRAND`	`41 44`	`4`
`TURN`	`45 48`	`4`
`STRAND`	`49 55`	`7`
`HELIX`	`58 60`	`3`
`HELIX`	`62 74`	`13`
`HELIX`	`76 86`	`11`
`STRAND`	`94 99`	`6`
`STRAND`	`104 106`	`3`

Sequence information

Length: 133 AA [This is the length of the unprocessed precursor]

Molecular weight: 15154 Da [This is the MW of the unprocessed precursor]

CRC64: C8EE4FBFD8C01F08 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAKEFGRPQR VAQEMQKEIA LILQREIKDP RLGMMTTVSG VEMSRDLAYA KVYVTFLNDK 

        70         80         90        100        110        120 
DEDAVKAGIK ALQEASGFIR SLLGKAMRLR IVPELTFFYD NSLVEGMRMS NLVTSVVKHD 

       130 
EERRVNPDDS KED

P0A7G2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools