[1]	PROTEIN SEQUENCE. PubMed=3768144 [NCBI, ExPASy, EBI, Israel, Japan] Dodt J., Machleidt W., Seemueller U., Maschler R., Fritz H.; "Isolation and characterization of hirudin isoinhibitors and sequence analysis of hirudin PA."; Biol. Chem. Hoppe-Seyler 367:803-811(1986).

Comments

FUNCTION: Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: Belongs to the protease inhibitor I14 (hirudin) family [view classification].

Copyright

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Cross-references

Sequence databases

PIR

A24350; A24350.

3D structure databases

SMR

P09944; 1-62.

ModBase

P09944.

Protein family/group databases

MEROPS

I14.001; -.

Ontologies

GO:0005576;	Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-KW).
GO:0004867;	Molecular function: serine-type endopeptidase inhibitor activity (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR000429; Prot_inh_hirudin.
Graphical view of domain structure.

Gene3D

G3DSA:2.70.10.10; Prot_inh_hirudin; 1.

Pfam

PF00713; Hirudin; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF001640; Hirudin; 1.

PRINTS

PR00777; HIRUDIN.

ProDom

PD004216; Prot_inh_hirudin; 1.
[Domain structure / List of seq. sharing at least 1 domain]

Other

P09944; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Glycoprotein; Protease inhibitor; Secreted; Serine protease inhibitor; Sulfation.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 66`	`66`	`Hirudin-PA.`	`PRO_0000195640`
`REGION`	`1 3`	`3`	`Interaction with thrombin active site (By similarity).`
`REGION`	`55 66`	`12`	`Interaction with fibrinogen-binding exosite of thrombin (By similarity).`
`MOD_RES`	`64 64`		`Sulfotyrosine.`
`CARBOHYD`	`45 45`		`O-linked (GalNAc...) (By similarity).`
`DISULFID`	`6 14`		`By similarity.`
`DISULFID`	`16 28`		`By similarity.`
`DISULFID`	`22 39`		`By similarity.`

Sequence information

Length: 66 AA [This is the length of the unprocessed precursor]

Molecular weight: 7026 Da [This is the MW of the unprocessed precursor]

CRC64: FA1B80B7F4FEA317 [This is a checksum on the sequence]

        10         20         30         40         50         60 
ITYTDCTESG QNLCLCEGSN VCGKGNKCIL GSQGKDNQCV TGEGTPKPQS HNQGDFEPIP 


EDAYDE

P09944 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools