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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC RNA], AND SEQUENCE REVISION.
Yamashiro T.,
Shameem G.,
Lai C.-J.;
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
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[2]
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NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Durbin A.P.,
Karron R.A.,
Sun W.,
Vaughn D.W.,
Reynolds M.J.,
Perreault J.R.,
Men R.H.,
Lai C.-J.,
Elkins W.R.,
Chanock R.M.,
Murphy B.R.,
Whitehead S.S.;
"A live attenuated dengue virus type 4 vaccine candidate with a 30 nucleotide deletion in the 3' untranslated region is highly attenuated and immunogenic in humans.";
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
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[3]
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NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-777.
DOI=10.1016/0042-6822(86)90169-8; PubMed=3022479 [NCBI, ExPASy, EBI, Israel, Japan]
Zhao B.,
Mackow E.,
Buckler-White A.,
Markoff L.,
Chancock R.M.,
Lai C.-J.,
Makino Y.;
"Cloning full-length dengue type 4 viral DNA sequences: analysis of genes coding for structural proteins.";
Virology 155:77-88(1986).
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[4]
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NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 775-3387.
DOI=10.1016/0042-6822(87)90458-2; PubMed=3039728 [NCBI, ExPASy, EBI, Israel, Japan]
Mackow E.,
Makino Y.,
Zhao B.,
Zhang Y.M.,
Markoff L.,
Buckler-White A.,
Guiler M.,
Chanock R.,
Lai C.-J.;
"The nucleotide sequence of dengue type 4 virus: analysis of genes coding for nonstructural proteins.";
Virology 159:217-228(1987).
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[5]
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PROTEOLYTIC PROCESSING OF THE M PROTEIN.
STRAIN=814669;
PubMed=2501515 [NCBI, ExPASy, EBI, Israel, Japan]
Markoff L.;
"In vitro processing of dengue virus structural proteins: cleavage of the pre-membrane protein.";
J. Virol. 63:3345-3352(1989).
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- FUNCTION: Protein C packages viral RNA to form a viral nucleocapsid, and promotes virion budding (By similarity).
- FUNCTION: prM acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is matured in the last step of virion assembly, presumably to avoid catastrophic activation of the viral fusion peptide induced by the acidic pH of the trans-Golgi network. After cleavage by host furin, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated (By similarity).
- FUNCTION: Envelope protein E binds cell surface receptor and is involved in membrane fusion between virion and target cell. Synthesized as an homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).
- FUNCTION: Non-structural protein 1 is slowly secreted from mammalian cells, but not from mosquito cells. Secreted form elicits protective immune response and plays an essential role in RNA replication. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome (By similarity).
- FUNCTION: Non-structural protein 2B is a required cofactor for the serine protease function of NS3 (By similarity).
- FUNCTION: Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction (By similarity).
- FUNCTION: Non-structural protein 4A plays a role in RNA replication. Enhances inhibition of cell antiviral response by non-structural protein 4B (By similarity).
- FUNCTION: Non-structural protein 4B prevent the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways (By similarity).
- FUNCTION: RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and assure the capping of genomes in the cytoplasm. May be involved in methylation of 5'RNA cap structure (By similarity).
- CATALYTIC ACTIVITY: Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
- SUBUNIT: prM and envelope protein E form heterodimers in the endoplasmic reticulum and Golgi. Envelope protein E forms homodimers. NS1 forms homodimers as well as homohexamers when secreted. NS1 may interact with NS4A. NS3 and NS2B form an heterodimer. NS3 interacts with unphosphorylated NS5 (By similarity).
- SUBCELLULAR LOCATION: Protein C: Virion (By similarity).
- SUBCELLULAR LOCATION: Peptide pr: Secreted (By similarity).
- SUBCELLULAR LOCATION: Small envelope protein M: Virion membrane; Single-pass type I membrane protein (By similarity).
- SUBCELLULAR LOCATION: Envelope protein E: Virion membrane; Single-pass type I membrane protein (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 1: Secreted. Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 2A-alpha: Endoplasmic reticulum membrane (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 2A: Endoplasmic reticulum membrane (By similarity).
- SUBCELLULAR LOCATION: Serine protease subunit NS2B: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
- SUBCELLULAR LOCATION: Serine protease subunit NS3: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 4A: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 4B: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=The C-terminal transmembrane domain of non-structural protein 4B is presumably reoriented after cleavage on the lumenal side (By similarity).
- SUBCELLULAR LOCATION: RNA-directed RNA polymerase NS5: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus (By similarity).
- DOMAIN: Transmembrane domains of the small envelope protein M and envelope protein E contains an endoplasmic reticulum retention signals (By similarity).
- PTM: Specific enzymatic cleavages in vivo yield mature proteins. The nascent protein C contains a C-terminal hydrophobic domain that act as a signal sequence for translocation of prM into the lumen of the ER. Mature protein C is cleaved at a site upstream of this hydrophobic domain by NS3. prM is cleaved in post-Golgi vesicles by a host furin, releasing the mature small envelope protein M, and peptide pr. Non-structural protein 2A-alpha, a C-terminally truncated form of non-structural protein 2A, results from partial cleavage by NS3 (By similarity).
- PTM: RNA-directed RNA polymerase NS5 is phosphorylated on serines residues. This phosphorylation may trigger NS5 nuclear localization (By similarity).
- PTM: Envelope protein E and non-structural protein 1 are N-glycosylated (By similarity).
- MISCELLANEOUS: The virion is assembled in the endoplasmic reticulum lumen, transported by vesicles to the Golgi, then transported again to the cell membrane where it is released outside the cell.
- SIMILARITY: Contains 1 helicase ATP-binding domain.
- SIMILARITY: Contains 1 helicase C-terminal domain.
- SIMILARITY: Contains 1 peptidase S7 domain [view classification].
- SIMILARITY: Contains 1 RdRp catalytic domain.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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