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UniProtKB/Swiss-Prot entry P09232


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PRTB_YEAST
Primary accession number P09232
Secondary accession numbers None
Integrated into Swiss-Prot on July 1, 1989
Sequence was last modified on July 1, 1989 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 93)
Name and origin of the protein
Protein name Cerevisin [Precursor]
Synonyms EC 3.4.21.48
Vacuolar protease B
Proteinase YSCB
Gene name
Name: PRB1
OrderedLocusNames: YEL060C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 281-295.
STRAIN=ATCC 204510 / AB320;
PubMed=3325823 [NCBI, ExPASy, EBI, Israel, Japan]
Moehle C.M., Tizard R., Lemmon S.K., Smart J., Jones E.W.;
"Protease B of the lysosomelike vacuole of the yeast Saccharomyces cerevisiae is homologous to the subtilisin family of serine proteases.";
Mol. Cell. Biol. 7:4390-4399(1987).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169868 [NCBI, ExPASy, EBI, Israel, Japan]
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
Nature 387:78-81(1997).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 605-635.
STRAIN=ATCC 204508 / S288c;
Saunders W.S., He L., Loo K.K., Hoyt M.;
Submitted (MAR-1992) to the EMBL/GenBank/DDBJ databases.
[4]
PROTEOLYTIC PROCESSING.
PubMed=1744078 [NCBI, ExPASy, EBI, Israel, Japan]
Nebes V.L., Jones E.W.;
"Activation of the proteinase B precursor of the yeast Saccharomyces cerevisiae by autocatalysis and by an internal sequence.";
J. Biol. Chem. 266:22851-22857(1991).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0701622104; PubMed=17563356 [NCBI, ExPASy, EBI, Israel, Japan]
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.";
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M18097; AAA34901.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U18795; AAB65027.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z11859; CAA77886.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M90522; AAA34495.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A29358; A29358.
RefSeq NP_010854.1; -.
3D structure databases
HSSP P06873; 1IC6. [HSSP ENTRY / SWISS-3DIMAGE / PDB]
ModBase P09232.
Protein-protein interaction databases
DIP DIP:2544N; -.
IntAct P09232; 19.
Protein family/group databases
MEROPS S08.052; -.
Organism-specific databases
CYGD YEL060c; -.
SGD S000000786; PRB1.
Yeast-GFP YEL060C.
Gene expression databases
ArrayExpress P09232; -.
GermOnline YEL060C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0000328; Cellular component: fungal-type vacuole lumen (traceable author statement from SGD).
GO:0042802; Molecular function: identical protein binding (inferred from electronic annotation from InterPro).
GO:0004252; Molecular function: serine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0009267; Biological process: cellular response to starvation (inferred from mutant phenotype from SGD).
GO:0043086; Biological process: negative regulation of catalytic activity (inferred from electronic annotation from InterPro).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
GO:0030435; Biological process: sporulation resulting in formation of a cellular spore (inferred from mutant phenotype from SGD).
GO:0007039; Biological process: vacuolar protein catabolic process (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR000209; Pept_S8_S53.
IPR015500; Peptidase_S8_subtilisin-rel.
IPR010259; Prot_inh_S8A.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.200; Pept_S8_S53; 1.
PANTHER PTHR10795; SubtilSerProt; 1.
Pfam PF00082; Peptidase_S8; 1.
PF05922; Subtilisin_N; 1.
Pfam graphical view of domain structure.
PRINTS PR00723; SUBTILISIN.
PROSITE PS00136; SUBTILASE_ASP; 1.
PS00137; SUBTILASE_HIS; 1.
PS00138; SUBTILASE_SER; 1.
Proteomic databases
PeptideAtlas P09232; -.
Proteomics databases
PRIDE P09232; -.
Genome annotation databases
Ensembl YEL060C; Saccharomyces cerevisiae. [Contig view]
GeneID 856649; -.
GenomeReviews U00092_GR; YEL060C.
KEGG sce:YEL060C; -.
NMPDR fig|4932.3.peg.1902; -.
Phylogenomic databases
HOGENOM P09232; -.
Other
LinkHub P09232; -.
NextBio 982625; -.
ProtoNet P09232.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; Glycoprotein; Hydrolase; Phosphoprotein; Protease; Serine protease; Signal; Vacuole; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    19  19     Potential. 
PROPEP   20   280  261      PRO_0000027136
CHAIN   281   635  355     Cerevisin. PRO_0000027137
ACT_SITE   325   325        Charge relay system (By similarity). 
ACT_SITE   357   357        Charge relay system (By similarity). 
ACT_SITE   519   519        Charge relay system (By similarity). 
MOD_RES   58    58        Phosphoserine. 
CARBOHYD   594   594        N-linked (GlcNAc...) (Probable). 
DISULFID   460   491        Potential. 
CONFLICT   622   622        F -> K (in Ref. 3; CAA77886/AAA34495). 
Sequence information
Length: 635 AA [This is the length of the unprocessed precursor] Molecular weight: 69621 Da [This is the MW of the unprocessed precursor] CRC64: C346C2B1C7DDDC48 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKLENTLFTL GALGSISAAL VIPNLENAAD HHELINKEDH HERPRKVEFT KDDDEEPSDS 

        70         80         90        100        110        120 
EDKEHGKFHK KGRKGQDKES PEFNGKRASG SHGSAHEGGK GMKPKHESSN DDDNDDKKKK 

       130        140        150        160        170        180 
PHHKGGCHEN KVEEKKMKGK KVKGKKHHEK TLEKGRHHNR LAPLVSTAQF NPDAISKIIP 

       190        200        210        220        230        240 
NRYIIVFKRG APQEEIDFHK ENVQQAQLQS VENLSAEDAF FISTKDTSLS TSEAGGIQDS 

       250        260        270        280        290        300 
FNIDNLFSGY IGYFTQEIVD LIRQNPLVDF VERDSIVEAT EFDTQNSAPW GLARISHRER 

       310        320        330        340        350        360 
LNLGSFNKYL YDDDAGRGVT SYVIDTGVNI NHKDFEKRAI WGKTIPLNDE DLDGNGHGTH 

       370        380        390        400        410        420 
CAGTIASKHY GVAKNANVVA VKVLRSNGSG TMSDVVKGVE YAAKAHQKEA QEKKKGFKGS 

       430        440        450        460        470        480 
TANMSLGGGK SPALDLAVNA AVEVGIHFAV AAGNENQDAC NTSPASADKA ITVGASTLSD 

       490        500        510        520        530        540 
DRAYFSNWGK CVDVFAPGLN ILSTYIGSDD ATATLSGTSM ASPHVAGLLT YFLSLQPGSD 

       550        560        570        580        590        600 
SEFFELGQDS LTPQQLKKKL IHYSTKDILF DIPEDTPNVL IYNGGGQDLS AFWNDTKKSH 

       610        620        630 
SSGFKQELNM DEFIGSKTDL IFDQVRDILD KLNII 

P09232 in FASTA format

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