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UniProtKB/Swiss-Prot entry P09118

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name URIC_RAT
Primary accession number P09118
Secondary accession numbers None
Integrated into Swiss-Prot on July 1, 1989
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    December 16, 2008 (Entry version 89)
Name and origin of the protein
Protein name Uricase
Synonyms EC 1.7.3.3
Urate oxidase
Gene name
Name: Uox
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE.
STRAIN=Sprague-Dawley;
TISSUE=Liver;
DOI=10.1016/0014-5793(90)80789-L; PubMed=2338140 [NCBI, ExPASy, EBI, Israel, Japan]
Motojima K., Goto S.;
"Organization of rat uricase chromosomal gene differs greatly from that of the corresponding plant gene.";
FEBS Lett. 264:156-158(1990).
[2]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Sprague-Dawley;
TISSUE=Liver;
PubMed=3182808 [NCBI, ExPASy, EBI, Israel, Japan]
Motojima K., Kanaya S., Goto S.;
"Cloning and sequence analysis of cDNA for rat liver uricase.";
J. Biol. Chem. 263:16677-16681(1988).
[3]
 NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Liver;
DOI=10.1016/0006-291X(89)92820-9; PubMed=2920046 [NCBI, ExPASy, EBI, Israel, Japan]
Alvares K., Nemali M.R., Reddy P.G., Wang X.D., Rao M.S., Reddy J.K.;
"The nucleotide sequence of a full length cDNA clone encoding rat liver urate oxidase.";
Biochem. Biophys. Res. Commun. 158:991-995(1989).
[4]
 NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
TISSUE=Liver;
PubMed=3267221 [NCBI, ExPASy, EBI, Israel, Japan]
Ito M., Suzuki M., Takagi Y.;
"Nucleotide sequence of cDNA and predicted amino acid sequence of rat liver uricase.";
Eur. J. Biochem. 173:459-463(1988).
[5]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(91)90055-G; PubMed=1999285 [NCBI, ExPASy, EBI, Israel, Japan]
Wang X.D., Kawano H., Alvares K., Reddy P.G., Getto H., Rao M.S., Reddy J.K.;
"Rat urate oxidase: cloning and structural analysis of the gene and 5'-flanking region.";
Gene 97:223-229(1991).
[6]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0888-7543(91)90013-5; PubMed=1783398 [NCBI, ExPASy, EBI, Israel, Japan]
Ito M., Nakamura M., Ogawa H., Kato S., Takagi Y.;
"Structural analysis of the gene encoding rat uricase.";
Genomics 11:905-913(1991).
[7]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Liver;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[8]
 NUCLEOTIDE SEQUENCE [MRNA] OF 11-303.
TISSUE=Liver;
PubMed=3194410 [NCBI, ExPASy, EBI, Israel, Japan]
Reddy P.G., Nemali M.R., Reddy M.K., Reddy M.N., Yuan P.M., Yuen S., Laffler T.G., Shiroza T., Kuramitsu H.K., Usuda N., Chisholm R.L., Rao M.S., Reddy J.K.;
"Isolation and sequence determination of a cDNA clone for rat peroxisomal urate oxidase: liver-specific expression in the rat.";
Proc. Natl. Acad. Sci. U.S.A. 85:9081-9085(1988).
[9]
 MUTAGENESIS OF LYS-164.
DOI=10.1016/S0006-291X(05)81464-0; PubMed=1520291 [NCBI, ExPASy, EBI, Israel, Japan]
Ito M., Kato S., Nakmura M., Go M., Takagi Y.;
"Identification of an amino acid residue involved in the substrate-binding site of rat liver uricase by site-directed mutagenesis.";
Biochem. Biophys. Res. Commun. 187:101-107(1992).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
J03959; AAA42318.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X13098; CAA31490.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M24396; AAA42317.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X07497; CAA30378.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M63593; AAA61699.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M63583; AAA61699.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M63586; AAA61699.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M63587; AAA61699.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M63588; AAA61699.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M63590; AAA61699.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M63591; AAA61699.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M63592; AAA61699.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC088112; AAH88112.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR I54070; RDRTU.
RefSeq NP_446220.1; -.
UniGene Rn.11330
3D structure databases
HSSP Q00511; 1UOX. [HSSP ENTRY / PDB]
ModBase P09118.
PTM databases
PhosphoSite P09118; -.
Organism-specific databases
RGD 621369; Uox.
Gene expression databases
ArrayExpress P09118; -.
GermOnline ENSRNOG00000016339; Rattus norvegicus.
Ontologies
GO
GO:0005777; Cellular component: peroxisome (inferred from direct assay from HGNC).
GO:0004846; Molecular function: urate oxidase activity (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006144; Biological process: purine base metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR002042; Uricase.
Graphical view of domain structure.
Gene3D G3DSA:3.10.270.10; Uricase; 1.
PANTHER PTHR10395:SF1; Uricase; 1.
Pfam PF01014; Uricase; 2.
Pfam graphical view of domain structure.
PRINTS PR00093; URICASE.
ProDom PD003367; Uricase; 2.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR03383; urate_oxi; 1.
PROSITE PS00366; URICASE; 1.
Proteomics databases
PRIDE P09118; -.
Genome annotation databases
Ensembl ENSRNOG00000016339; Rattus norvegicus. [Contig view]
GeneID 114768; -.
KEGG rno:114768; -.
Phylogenomic databases
HOVERGEN P09118; -.
Other
NextBio 618867; -.
ProtoNet P09118.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Direct protein sequencing; Oxidoreductase; Peroxisome; Phosphoprotein; Purine metabolism.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   303  302     Uricase. PRO_0000165990
MOTIF   301   303  3     Microbody targeting signal (Potential). 
ACT_SITE   187   187        Charge relay system (By similarity). 
ACT_SITE   235   235        Charge relay system (By similarity). 
MOD_RES   2     2        N-acetylalanine (By similarity). 
MOD_RES   36    36        N6-acetyllysine (By similarity). 
MOD_RES   39    39        Phosphoserine (By similarity). 
MOD_RES   55    55        N6-acetyllysine (By similarity). 
MOD_RES   118   118        N6-acetyllysine (By similarity). 
MOD_RES   122   122        N6-acetyllysine (By similarity). 
MOD_RES   164   164        N6-acetyllysine (By similarity). 
MOD_RES   185   185        N6-acetyllysine (By similarity). 
MOD_RES   220   220        N6-acetyllysine (By similarity). 
MOD_RES   231   231        Phosphoserine (By similarity). 
MOD_RES   277   277        N6-acetyllysine (By similarity). 
MOD_RES   290   290        N6-acetyllysine (By similarity). 
MOD_RES   301   301        Phosphoserine (By similarity). 
MUTAGEN   164   164        K->E,I: Loss of activity. 
CONFLICT   55    55        K -> D (in Ref. 6). 
CONFLICT   111   111        Y -> H (in Ref. 3; AAA42317 and 6). 
Sequence information
Length: 303 AA [This is the length of the unprocessed precursor] Molecular weight: 34934 Da [This is the MW of the unprocessed precursor] CRC64: 14CFF2F4E18C7BBB [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAHYHDDYGK NDEVEFVRTG YGKDMVKVLH IQRDGKYHSI KEVATSVQLT LRSKKDYLHG 

        70         80         90        100        110        120 
DNSDIIPTDT IKNTVHVLAK FKGIKSIETF AMNICEHFLS SFSHVTRAHV YVEEVPWKRF 

       130        140        150        160        170        180 
EKNGVKHVHA FIHTPTGTHF CDVEQVRNGP PIIHSGIKDL KVLKTTQSGF EGFIKDQFTT 

       190        200        210        220        230        240 
LPEVKDRCFA TQVYCKWRYQ NRDVDFEATW GAVRDIVLKK FAGPYDRGEY SPSVQKTLYD 

       250        260        270        280        290        300 
IQVLTLSQLP EIEDMEISLP NIHYFNIDMS KMGLINKEEV LLPLDNPYGK ITGTVRRKLP 


SRL
P09118 in FASTA format

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