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UniProtKB/Swiss-Prot entry P08660


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name AK3_ECOLI
Primary accession number P08660
Secondary accession number Q2M6T0
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on October 1, 1993 (Sequence version 2)
Annotations were last modified on    December 16, 2008 (Entry version 83)
Name and origin of the protein
Protein name Lysine-sensitive aspartokinase 3
Synonyms EC 2.7.2.4
Lysine-sensitive aspartokinase III
Aspartate kinase III
Gene name
Name: lysC
Synonyms: apk
OrderedLocusNames: b4024, JW3984
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
PubMed=3003049 [NCBI, ExPASy, EBI, Israel, Japan]
Cassan M., Parsot C., Cohen G.N., Patte J.-C.;
"Nucleotide sequence of lysC gene encoding the lysine-sensitive aspartokinase III of Escherichia coli K12. Evolutionary pathway leading to three isofunctional enzymes.";
J. Biol. Chem. 261:1052-1057(1986).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1093/nar/21.23.5408; PubMed=8265357 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.;
"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.";
Nucleic Acids Res. 21:5408-5417(1993).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
DOI=10.1093/nar/11.18.6157; PubMed=6312411 [NCBI, ExPASy, EBI, Israel, Japan]
Cassan M., Ronceray J., Patte J.-C.;
"Nucleotide sequence of the promoter region of the E. coli lysC gene.";
Nucleic Acids Res. 11:6157-6166(1983).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M11812; AAA24095.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00006; AAC43118.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC76994.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE78026.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X00008; CAA24910.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR G65209; KIECD3.
RefSeq AP_004525.1; -.
NP_418448.1; -.
3D structure databases
PDB
2J0W; X-ray; 2.50 A; A=1-449.[ExPASy / RCSB / EBI]
2J0X; X-ray; 2.80 A; A/B=1-449.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 2J0W; -.
2J0X; -.
ModBase P08660.
Enzyme and pathway databases
BioCyc EcoCyc:ASPKINIII-MON; -.
MetaCyc:ASPKINIII-MON; -.
Organism-specific databases
EchoBASE EB0545; -.
EcoGene EG10550; lysC.
Ontologies
GO
GO:0016597; Molecular function: amino acid binding (inferred from electronic annotation from InterPro).
GO:0004072; Molecular function: aspartate kinase activity (inferred from electronic annotation from InterPro).
GO:0009085; Biological process: lysine biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR002912; ACT_bd.
IPR001048; Asp/Glu/Uridylate_kinase.
IPR005260; Asp_kin_monofn.
IPR001341; Asp_kin_reg.
Graphical view of domain structure.
Gene3D G3DSA:3.40.1160.10; Aa_kinase; 1.
Pfam PF00696; AA_kinase; 1.
PF01842; ACT; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00656; asp_kin_monofn; 1.
TIGR00657; asp_kinases; 1.
PROSITE PS00324; ASPARTOKINASE; 1.
Genome annotation databases
GeneID 948531; -.
GenomeReviews AP009048_GR; JW3984.
U00096_GR; b4024.
KEGG ecj:JW3984; -.
eco:b4024; -.
Phylogenomic databases
HOGENOM P08660; -.
Other
LinkHub P08660; -.
Genome annotation databases
CMR P08660; b4024.
Other
ProtoNet P08660.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Amino-acid biosynthesis; Complete proteome; Kinase; Lysine biosynthesis; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   449  449     Lysine-sensitive aspartokinase 3. PRO_0000066676
DOMAIN   309   386  78     ACT. 
REGION   1   245  245     Aspartokinase. 
REGION   246   449  204     Interface. 
CONFLICT   58    58        G -> C (in Ref. 1; AAA24095). 
CONFLICT   401   401        G -> A (in Ref. 1; AAA24095). 
HELIX   68    82  15      
STRAND   87    90  4      
HELIX   93   103  11      
HELIX   109   119  11      
HELIX   122   133  12      
TURN   150   152  3      
HELIX   161   168  8      
STRAND   176   179  4      
STRAND   181   186  6      
STRAND   190   192  3      
STRAND   196   198  3      
HELIX   201   212  12      
STRAND   216   220  5      
STRAND   222   231  10      
HELIX   236   245  10      
HELIX   250   264  15      
STRAND   269   277  9      
Sequence information
Length: 449 AA [This is the length of the unprocessed precursor] Molecular weight: 48532 Da [This is the MW of the unprocessed precursor] CRC64: 5B41CE3A6E4D984B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSEIVVSKFG GTSVADFDAM NRSADIVLSD ANVRLVVLSA SAGITNLLVA LAEGLEPGER 

        70         80         90        100        110        120 
FEKLDAIRNI QFAILERLRY PNVIREEIER LLENITVLAE AAALATSPAL TDELVSHGEL 

       130        140        150        160        170        180 
MSTLLFVEIL RERDVQAQWF DVRKVMRTND RFGRAEPDIA ALAELAALQL LPRLNEGLVI 

       190        200        210        220        230        240 
TQGFIGSENK GRTTTLGRGG SDYTAALLAE ALHASRVDIW TDVPGIYTTD PRVVSAAKRI 

       250        260        270        280        290        300 
DEIAFAEAAE MATFGAKVLH PATLLPAVRS DIPVFVGSSK DPRAGGTLVC NKTENPPLFR 

       310        320        330        340        350        360 
ALALRRNQTL LTLHSLNMLH SRGFLAEVFG ILARHNISVD LITTSEVSVA LTLDTTGSTS 

       370        380        390        400        410        420 
TGDTLLTQSL LMELSALCRV EVEEGLALVA LIGNDLSKAC GVGKEVFGVL EPFNIRMICY 

       430        440 
GASSHNLCFL VPGEDAEQVV QKLHSNLFE 

P08660 in FASTA format

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