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UniProtKB/Swiss-Prot entry P08659


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LUCI_PHOPY
Primary accession number P08659
Secondary accession number Q27755
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on January 1, 1988 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 68)
Name and origin of the protein
Protein name Luciferin 4-monooxygenase
Synonyms Luciferase
EC 1.13.12.7
Gene name None
From
Photinus pyralis (North American firefly) [TaxID: 7054] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Elateriformia; Elateroidea; Lampyridae; Lampyrinae; Photinus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3821727 [NCBI, ExPASy, EBI, Israel, Japan]
de Wet J.R., Wood K.V., Deluca M., Helinski D.R., Subramani S.;
"Firefly luciferase gene: structure and expression in mammalian cells.";
Mol. Cell. Biol. 7:725-737(1987).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Croizier G.M.J.;
"Construction and utilization of an Autographa californica nuclear polyhedrosis virus vector with a unique cloning site: expression of genes amplified by the polymerase chain reaction.";
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
[3]
SUBCELLULAR LOCATION.
PubMed=3554235 [NCBI, ExPASy, EBI, Israel, Japan]
Keller G.-A., Gould S., de Luca M., Subramani S.;
"Firefly luciferase is targeted to peroxisomes in mammalian cells.";
Proc. Natl. Acad. Sci. U.S.A. 84:3264-3268(1987).
[4]
X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
DOI=10.1016/S0969-2126(96)00033-0; PubMed=8805533 [NCBI, ExPASy, EBI, Israel, Japan]
Conti E., Franks N.P., Brick P.;
"Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes.";
Structure 4:287-298(1996).
[5]
X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
PubMed=9788915 [NCBI, ExPASy, EBI, Israel, Japan]
Franks N.P., Jenkins A., Conti E., Lieb W.R., Brick P.;
"Structural basis for the inhibition of firefly luciferase by a general anesthetic.";
Biophys. J. 75:2205-2211(1998).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M15077; AAA29795.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X84846; CAA59281.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X84848; CAA59283.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U03687; AAA03561.1; -; Unassigned_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U89934; AAB64396.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U89935; AAB64399.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A26772; A26772.
3D structure databases
PDB
1BA3; X-ray; 2.20 A; A=1-550.[ExPASy / RCSB / EBI]
1LCI; X-ray; 2.00 A; A=1-550.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1BA3; -.
1LCI; -.
ModBase P08659.
Ontologies
GO
GO:0005777; Cellular component: peroxisome (inferred from direct assay from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR000873; AMP-dep_Synth/Lig.
Graphical view of domain structure.
Pfam PF00501; AMP-binding; 1.
Pfam graphical view of domain structure.
PRINTS PR00154; AMPBINDING.
PROSITE PS00455; AMP_BINDING; 1.
BLOCKS P08659.
Other
LinkHub P08659; -.
ProtoNet P08659.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Luminescence; Magnesium; Metal-binding; Monooxygenase; Oxidoreductase; Peroxisome; Photoprotein.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   550  550     Luciferin 4-monooxygenase. PRO_0000193147
MOTIF   548   550  3     Microbody targeting signal. 
CONFLICT   361   361        A -> G (in Ref. 2; CAA59281). 
STRAND   7     9  3      
HELIX   22    34  13      
STRAND   40    44  5      
TURN   45    47  3      
STRAND   50    52  3      
HELIX   53    70  18      
STRAND   77    81  5      
HELIX   89    98  10      
STRAND   101   104  4      
HELIX   111   121  11      
STRAND   124   128  5      
HELIX   130   132  3      
HELIX   133   142  10      
STRAND   148   153  6      
STRAND   161   163  3      
HELIX   164   171  8      
TURN   178   180  3      
TURN   188   190  3      
STRAND   191   197  7      
STRAND   207   211  5      
HELIX   212   222  11      
TURN   225   227  3      
STRAND   236   239  4      
HELIX   246   258  13      
STRAND   261   264  4      
HELIX   270   279  10      
STRAND   283   286  4      
HELIX   289   297  9      
HELIX   301   303  3      
STRAND   311   313  3      
HELIX   321   330  10      
STRAND   339   341  3      
HELIX   343   345  3      
STRAND   346   351  6      
STRAND   363   365  3      
STRAND   370   374  5      
TURN   376   378  3      
STRAND   388   394  7      
STRAND   399   401  3      
HELIX   405   411  7      
STRAND   418   426  9      
STRAND   432   434  3      
HELIX   437   439  3      
STRAND   442   444  3      
STRAND   447   449  3      
HELIX   451   459  9      
STRAND   464   474  11      
TURN   475   477  3      
STRAND   478   487  10      
HELIX   495   503  9      
STRAND   515   518  4      
STRAND   528   530  3      
HELIX   532   542  11      
Sequence information
Length: 550 AA [This is the length of the unprocessed precursor] Molecular weight: 60745 Da [This is the MW of the unprocessed precursor] CRC64: E380FCE9D56ACCDE [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN ITYAEYFEMS 

        70         80         90        100        110        120 
VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV AVAPANDIYN ERELLNSMNI 

       130        140        150        160        170        180 
SQPTVVFVSK KGLQKILNVQ KKLPIIQKII IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD 

       190        200        210        220        230        240 
FVPESFDRDK TIALIMNSSG STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV 

       250        260        270        280        290        300 
VPFHHGFGMF TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL 

       310        320        330        340        350        360 
IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL ITPEGDDKPG 

       370        380        390        400        410        420 
AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG YVNNPEATNA LIDKDGWLHS 

       430        440        450        460        470        480 
GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA PAELESILLQ HPNIFDAGVA GLPDDDAGEL 

       490        500        510        520        530        540 
PAAVVVLEHG KTMTEKEIVD YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI 

       550 
KAKKGGKSKL 

P08659 in FASTA format

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