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UniProtKB/Swiss-Prot entry P08218


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ELA2B_HUMAN
Primary accession number P08218
Secondary accession numbers Q14D16 Q6ISM5 Q96QV5
Integrated into Swiss-Prot on August 1, 1988
Sequence was last modified on July 22, 2008 (Sequence version 2)
Annotations were last modified on    December 16, 2008 (Entry version 82)
Name and origin of the protein
Protein name Elastase-2B [Precursor]
Synonym EC 3.4.21.71
Gene name
Name: ELA2B
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS ARG-79; ASN-114 AND ARG-177.
PubMed=3646943 [NCBI, ExPASy, EBI, Israel, Japan]
Kawashima I., Tani T., Shimoda K., Takiguchi Y.;
"Characterization of pancreatic elastase II cDNAs: two elastase II mRNAs are expressed in human pancreas.";
DNA 6:163-172(1987).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature04727; PubMed=16710414 [NCBI, ExPASy, EBI, Israel, Japan]
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
"The DNA sequence and biological annotation of human chromosome 1.";
Nature 441:315-321(2006).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.;
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS ARG-79; ASN-114 AND ARG-177.
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M16653; AAA52381.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL512883; CAC42422.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CH471167; EAW51728.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC069455; AAH69455.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC113540; AAI13541.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC113542; AAI13543.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR C26823; C26823.
UniGene Hs.631871
3D structure databases
HSSP P08419; 1BRU. [HSSP ENTRY / PDB]
SMR P08218; 29-269.
ModBase P08218.
Protein family/group databases
MEROPS S01.206; -.
Organism-specific databases
GeneCards GC01P015675; -.
MIM 609444; gene. [NCBI / EBI]
GeneCards P08218.
Gene expression databases
ArrayExpress P08218; -.
GermOnline ENSG00000142615; Homo sapiens.
Ontologies
GO
GO:0005576; Cellular component: extracellular region (traceable author statement from ProtInc).
GO:0004252; Molecular function: serine-type endopeptidase activity (traceable author statement from ProtInc).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001254; Peptidase_S1_S6.
IPR001314; Peptidase_S1A.
Graphical view of domain structure.
Pfam PF00089; Trypsin; 1.
Pfam graphical view of domain structure.
PRINTS PR00722; CHYMOTRYPSIN.
SMART SM00020; Tryp_SPc; 1.
SMART graphical view of domain structure.
PROSITE PS50240; TRYPSIN_DOM; 1.
PS00134; TRYPSIN_HIS; 1.
PS00135; TRYPSIN_SER; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
Ensembl ENSG00000215704; Homo sapiens. [Contig view]
Phylogenomic databases
HOVERGEN P08218; -.
Other
SOURCE ELA2B; Homo sapiens.
ProtoNet P08218.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Hydrolase; Polymorphism; Protease; Secreted; Serine protease; Signal; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    16  16      
PROPEP   17    28  12     Activation peptide. PRO_0000027695
CHAIN   29   269  241     Elastase-2B. PRO_0000027696
DOMAIN   29   267  239     Peptidase S1. 
ACT_SITE   73    73        Charge relay system (By similarity). 
ACT_SITE   121   121        Charge relay system (By similarity). 
ACT_SITE   216   216        Charge relay system (By similarity). 
DISULFID   58    74        By similarity. 
DISULFID   155   222        By similarity. 
DISULFID   186   202        By similarity. 
DISULFID   212   243        By similarity. 
VARIANT   79    79  1     G -> R (in dbSNP:rs3820071 [NCBI]). VAR_044534 [3D]
VARIANT   114   114  1     D -> N (in dbSNP:rs3766160 [NCBI]). VAR_044535 [3D]
VARIANT   177   177  1     Q -> R (in dbSNP:rs6429745 [NCBI]). VAR_044536 [3D]
VARIANT   235   235  1     G -> S (in dbSNP:rs3737703 [NCBI]). VAR_044537 [3D]
Sequence information
Length: 269 AA [This is the length of the unprocessed precursor] Molecular weight: 28810 Da [This is the MW of the unprocessed precursor] CRC64: CC81C1D18B918B5F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MIRTLLLSTL VAGALSCGVS TYAPDMSRML GGEEARPNSW PWQVSLQYSS NGQWYHTCGG 

        70         80         90        100        110        120 
SLIANSWVLT AAHCISSSGI YRVMLGQHNL YVAESGSLAV SVSKIVVHKD WNSDQVSKGN 

       130        140        150        160        170        180 
DIALLKLANP VSLTDKIQLA CLPPAGTILP NNYPCYVTGW GRLQTNGALP DDLKQGQLLV 

       190        200        210        220        230        240 
VDYATCSSSG WWGSTVKTNM ICAGGDGVIC TCNGDSGGPL NCQASDGRWE VHGIGSLTSV 

       250        260 
LGCNYYYKPS IFTRVSNYND WINSVIANN 

P08218 in FASTA format

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