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UniProtKB/Swiss-Prot entry P08217


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ELA2A_HUMAN
Primary accession number P08217
Secondary accession number Q14243
Integrated into Swiss-Prot on August 1, 1988
Sequence was last modified on August 1, 1988 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 86)
Name and origin of the protein
Protein name Elastase-2A [Precursor]
Synonym EC 3.4.21.71
Gene name
Name: ELA2A
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1021/bi00397a010; PubMed=3427074 [NCBI, ExPASy, EBI, Israel, Japan]
Fletcher T.S., Shen W.F., Largman C.;
"Primary structure of human pancreatic elastase 2 determined by sequence analysis of the cloned mRNA.";
Biochemistry 26:7256-7261(1987).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=3646943 [NCBI, ExPASy, EBI, Israel, Japan]
Kawashima I., Tani T., Shimoda K., Takiguchi Y.;
"Characterization of pancreatic elastase II cDNAs: two elastase II mRNAs are expressed in human pancreas.";
DNA 6:163-172(1987).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Pancreas;
PubMed=2834346 [NCBI, ExPASy, EBI, Israel, Japan]
Shirasu Y., Yoshida H., Matsuki S., Takemura K., Ikeda N., Shimada Y., Ozawa T., Mikayama T., Iijima H., Ishida A., Sato Y., Tamai Y., Tanaka J., Ikenaga H.;
"Molecular cloning and expression in Escherichia coli of a cDNA encoding human pancreatic elastase 2.";
J. Biochem. 102:1555-1563(1987).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature04727; PubMed=16710414 [NCBI, ExPASy, EBI, Israel, Japan]
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
"The DNA sequence and biological annotation of human chromosome 1.";
Nature 441:315-321(2006).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Pancreas;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[6]
PROTEIN SEQUENCE OF 18-50, AND INTERACTION WITH CPA1.
DOI=10.1016/0014-5793(90)80665-6; PubMed=2307232 [NCBI, ExPASy, EBI, Israel, Japan]
Moulard M., Michon T., Kerfelec B., Chapus C.;
"Further studies on the human pancreatic binary complexes involving procarboxypeptidase A.";
FEBS Lett. 261:179-183(1990).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M16631; AAA52374.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M16652; AAA52380.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D00236; BAA00165.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL512883; CAC42421.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC007031; AAH07031.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B26823; B26823.
RefSeq NP_254275.1; -.
UniGene Hs.631866
3D structure databases
HSSP P08419; 1BRU. [HSSP ENTRY / PDB]
SMR P08217; 29-269.
ModBase P08217.
Protein family/group databases
MEROPS S01.155; -.
Organism-specific databases
GeneCards GC01P015655; -.
H-InvDB HIX0000152; -.
MIM 609443; gene. [NCBI / EBI]
GeneCards P08217.
Gene expression databases
ArrayExpress P08217; -.
GermOnline ENSG00000142615; Homo sapiens.
Ontologies
GO
GO:0005576; Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-KW).
GO:0004252; Molecular function: serine-type endopeptidase activity (traceable author statement from ProtInc).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001254; Peptidase_S1_S6.
IPR001314; Peptidase_S1A.
Graphical view of domain structure.
Pfam PF00089; Trypsin; 1.
Pfam graphical view of domain structure.
PRINTS PR00722; CHYMOTRYPSIN.
SMART SM00020; Tryp_SPc; 1.
SMART graphical view of domain structure.
PROSITE PS50240; TRYPSIN_DOM; 1.
PS00134; TRYPSIN_HIS; 1.
PS00135; TRYPSIN_SER; 1.
PROSITE graphical view of domain structure (profiles).
Proteomics databases
PRIDE P08217; -.
Genome annotation databases
Ensembl ENSG00000142615; Homo sapiens. [Contig view]
GeneID 63036; -.
KEGG hsa:63036; -.
Phylogenomic databases
HOVERGEN P08217; -.
Other
LinkHub P08217; -.
NextBio 65535; -.
SOURCE ELA2A; Homo sapiens.
ProtoNet P08217.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Direct protein sequencing; Hydrolase; Polymorphism; Protease; Secreted; Serine protease; Signal; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    16  16      
PROPEP   17    28  12     Activation peptide. PRO_0000027693
CHAIN   29   269  241     Elastase-2A. PRO_0000027694
DOMAIN   29   267  239     Peptidase S1. 
ACT_SITE   73    73        Charge relay system (By similarity). 
ACT_SITE   121   121        Charge relay system (By similarity). 
ACT_SITE   216   216        Charge relay system (By similarity). 
DISULFID   58    74        By similarity. 
DISULFID   155   222        By similarity. 
DISULFID   186   202        By similarity. 
DISULFID   212   243        By similarity. 
VARIANT   257   257  1     N -> S (in dbSNP:rs2303193 [NCBI]). VAR_051837 [3D]
CONFLICT   202   202        C -> V (in Ref. 3; BAA00165). 
Sequence information
Length: 269 AA [This is the length of the unprocessed precursor] Molecular weight: 28888 Da [This is the MW of the unprocessed precursor] CRC64: A2E05143EFF4987C [This is a checksum on the sequence]
        10         20         30         40         50         60 
MIRTLLLSTL VAGALSCGDP TYPPYVTRVV GGEEARPNSW PWQVSLQYSS NGKWYHTCGG 

        70         80         90        100        110        120 
SLIANSWVLT AAHCISSSRT YRVGLGRHNL YVAESGSLAV SVSKIVVHKD WNSNQISKGN 

       130        140        150        160        170        180 
DIALLKLANP VSLTDKIQLA CLPPAGTILP NNYPCYVTGW GRLQTNGAVP DVLQQGRLLV 

       190        200        210        220        230        240 
VDYATCSSSA WWGSSVKTSM ICAGGDGVIS SCNGDSGGPL NCQASDGRWQ VHGIVSFGSR 

       250        260 
LGCNYYHKPS VFTRVSNYID WINSVIANN 

P08217 in FASTA format

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