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UniProtKB/Swiss-Prot entry P08066


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHSB_BACSU
Primary accession number P08066
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1988
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    November 25, 2008 (Entry version 84)
Name and origin of the protein
Protein name Succinate dehydrogenase iron-sulfur subunit
Synonym EC 1.3.99.1
Gene name
Name: sdhB
OrderedLocusNames: BSU28430
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168 / PY79;
PubMed=3027051 [NCBI, ExPASy, EBI, Israel, Japan]
Phillips M.K., Hederstedt L., Hasnain S., Rutberg L., Guest J.R.;
"Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex.";
J. Bacteriol. 169:864-873(1987).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
PubMed=8969504 [NCBI, ExPASy, EBI, Israel, Japan]
Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R.;
"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism.";
Microbiology 142:3067-3078(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 112-253.
PubMed=3114423 [NCBI, ExPASy, EBI, Israel, Japan]
Cutting S.M., Mandelstam J.;
"The nucleotide sequence and the transcription during sporulation of the gerE gene of Bacillus subtilis.";
J. Gen. Microbiol. 132:3013-3024(1986).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M13470; AAA22747.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z75208; CAA99548.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99118; CAB14803.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M17642; AAA22471.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B27763; B27763.
RefSeq NP_390721.1; -.
3D structure databases
HSSP P17596; 1QLA. [HSSP ENTRY / PDB]
ModBase P08066.
Enzyme and pathway databases
BioCyc BSUB224308:BSU2839-MON; -.
Organism-specific databases
SubtiList BG10353; sdhB. [Micado]
Ontologies
GO
GO:0051537; Molecular function: 2 iron, 2 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051538; Molecular function: 3 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0000104; Molecular function: succinate dehydrogenase activity (inferred from electronic annotation from EC).
GO:0022900; Biological process: electron transport chain (inferred from electronic annotation from UniProtKB-KW).
GO:0006810; Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
GO:0006099; Biological process: tricarboxylic acid cycle (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001450; 4Fe4S_Fe_S_bd.
IPR012675; b-grasp_ferredoxin-like.
IPR012285; Fum_reductase_C.
IPR004489; Succ_DHase/fum_Rdtase_Fe-S.
Graphical view of domain structure.
Gene3D G3DSA:3.10.20.30; Ferredoxin_fold; 1.
G3DSA:1.10.1060.10; Fum_reductase_C; 1.
Pfam PF00037; Fer4; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00384; dhsB; 1.
PROSITE PS00198; 4FE4S_FER_1; 1.
PS51379; 4FE4S_FER_2; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
GeneID 937460; -.
GenomeReviews AL009126_GR; BSU28430.
KEGG bsu:BSU28430; -.
NMPDR fig|224308.1.peg.2846; -.
Phylogenomic databases
HOGENOM P08066; -.
Genome annotation databases
CMR P08066; BSU28430.
Other
ProtoNet P08066.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
2Fe-2S; 3Fe-4S; 4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Transport; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   253  252     Succinate dehydrogenase iron-sulfur subunit. PRO_0000158686
DOMAIN   146   174  29     4Fe-4S ferredoxin-type. 
METAL   64    64        Iron-sulfur 1 (2Fe-2S) (By similarity). 
METAL   69    69        Iron-sulfur 1 (2Fe-2S) (By similarity). 
METAL   72    72        Iron-sulfur 1 (2Fe-2S) (By similarity). 
METAL   84    84        Iron-sulfur 1 (2Fe-2S) (By similarity). 
METAL   155   155        Iron-sulfur 2 (4Fe-4S) (By similarity). 
METAL   158   158        Iron-sulfur 2 (4Fe-4S) (By similarity). 
METAL   161   161        Iron-sulfur 2 (4Fe-4S) (By similarity). 
METAL   165   165        Iron-sulfur 3 (3Fe-4S) (By similarity). 
METAL   212   212        Iron-sulfur 3 (3Fe-4S) (By similarity). 
METAL   218   218        Iron-sulfur 3 (3Fe-4S) (By similarity). 
METAL   222   222        Iron-sulfur 2 (4Fe-4S) (By similarity). 
Sequence information
Length: 253 AA [This is the length of the unprocessed precursor] Molecular weight: 28418 Da [This is the MW of the unprocessed precursor] CRC64: C20E095CDD960B3E [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSEQKTIRFI ITRQDTADST PYDEEFEIPY RPNLNVISAL MEIRRNPVNV KGEKTTPVTW 

        70         80         90        100        110        120 
DMNCLEEVCG ACSMVINGKP RQSCTALIDQ LEQPIRLKPM KTFPVVRDLQ VDRSRMFDSL 

       130        140        150        160        170        180 
KKVKAWIPID GTYDLGPGPR MPEKRRQWAY ELSKCMTCGV CLEACPNVNS KSKFMGPAPM 

       190        200        210        220        230        240 
SQVRLFNAHP TGAMNKSERL EALMDEGGLA DCGNSQNCVQ SCPKGIPLTT SIAALNRDTN 

       250 
LQAFRNFFGS DRV 

P08066 in FASTA format

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