[1]	NUCLEOTIDE SEQUENCE [MRNA] (C ALLELE; ISOFORM 2). DOI=10.1016/S0300-9084(85)80291-1; PubMed=3002501 [NCBI, ExPASy, EBI, Israel, Japan] Mercier J.-C., Gaye P., Soulier S., Hue-Delahaie D., Vilotte J.-L.; "Construction and identification of recombinant plasmids carrying cDNAs coding for ovine alpha S1-, alpha S2-, beta-, kappa-casein and beta-lactoglobulin. Nucleotide sequence of alpha S1-casein cDNA."; Biochimie 67:959-971(1985).
[2]	PROTEIN SEQUENCE OF 16-214 (A; C AND D ALLELES; ISOFORMS 1 AND 2), PHOSPHORYLATION AT SER-27; SER-56; SER-61; SER-63; SER-79; SER-80; SER-81; SER-82; SER-83; SER-90 AND SER-130, AND MASS SPECTROMETRY. TISSUE=Milk; PubMed=7601973 [NCBI, ExPASy, EBI, Israel, Japan] Ferranti P., Malorni A., Nitti G., Laezza P., Pizzano R., Chianese L., Addeo F.; "Primary structure of ovine alpha s1-caseins: localization of phosphorylation sites and characterization of genetic variants A, C and D."; J. Dairy Res. 62:281-296(1995).
[3]	VARIANT THR-209. STRAIN=Comisana, Sarda, and Sopravissana; TISSUE=Blood; PubMed=15328285 [NCBI, ExPASy, EBI, Israel, Japan] Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E., Caroli A.; "Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism."; J. Dairy Sci. 87:2606-2613(2004).

Comments

FUNCTION: Important role in the capacity of milk to transport calcium phosphate.
SUBCELLULAR LOCATION: Secreted.

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	1
Synonyms	Long
Isoform ID	P04653-2
This is the isoform sequence displayed in this entry.

Name	2
Synonyms	Short
Isoform ID	P04653-1
Features which should be applied to build the isoform sequence: VSP_012408.

TISSUE SPECIFICITY: Mammary gland specific. Secreted in milk.
MASS SPECTROMETRY: Mass=23638.14; Mass_error=3.0; Method=Electrospray; Range=16-214 (P04653-2); Note=Allele A, with 11 phosphate groups; Source=PubMed:7601973;.
MASS SPECTROMETRY: Mass=22620.0; Mass_error=1.6; Method=Electrospray; Range=16-214 (P04653-1); Note=Allele A, with 11 phosphate groups; Source=PubMed:7601973;.
MASS SPECTROMETRY: Mass=23561.8; Mass_error=2.3; Method=Electrospray; Range=16-214 (P04653-2); Note=Allele C, with 10 phosphate groups; Source=PubMed:7601973;.
MASS SPECTROMETRY: Mass=22551.8; Mass_error=2.2; Method=Electrospray; Range=16-214 (P04653-1); Note=Allele C, with 10 phosphate groups; Source=PubMed:7601973;.
MASS SPECTROMETRY: Mass=23290.2; Mass_error=2.9; Method=Electrospray; Range=16-214 (P04653-2); Note=Allele D, with 6 phosphate groups; Source=PubMed:7601973;.
MASS SPECTROMETRY: Mass=22629.0; Mass_error=1.6; Method=Electrospray; Range=16-214 (P04653-1); Note=Allele D, with 6 phosphate groups; Source=PubMed:7601973;.
POLYMORPHISM: At least four alleles are known. The sequence shown is that of allele A. The frequency of variant Thr-209 is 0.263, 0.122 and 0.191 in Italian breeds Comisana, Sarda and Sopravissana, respectively.
SIMILARITY: Belongs to the alpha-casein family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X03237; CAA26982.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY444506; AAS17944.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

A25069; KASHS1.

NP_001009795.1; -.

3D structure databases

ModBase

P04653.

Family and domain databases

InterPro

IPR001588; Casein.
Graphical view of domain structure.

Pfam

PF00363; Casein; 1.
Pfam graphical view of domain structure.

PROSITE

PS00306; CASEIN_ALPHA_BETA; 1.

BLOCKS

P04653.

Genome annotation databases

GeneID

443382; -.

Phylogenomic databases

HOVERGEN

P04653; -.

Other

ProtoNet

P04653.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Direct protein sequencing; Phosphoprotein; Polymorphism; Secreted; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 15`	`15`
`CHAIN`	`16 214`	`199`	`Alpha-S1-casein.`	`PRO_0000004459`
`REGION`	`105 111`	`7`	`Opioid-like peptide sequence.`
`MOD_RES`	`27 27`		`Phosphoserine; in allele A.`
`MOD_RES`	`56 56`		`Phosphoserine; in allele C.`
`MOD_RES`	`61 61`		`Phosphoserine.`
`MOD_RES`	`63 63`		`Phosphoserine.`
`MOD_RES`	`79 79`		`Phosphoserine; in alleles A and C.`
`MOD_RES`	`80 80`		`Phosphoserine.`
`MOD_RES`	`81 81`		`Phosphoserine; in alleles A and C.`
`MOD_RES`	`82 82`		`Phosphoserine.`
`MOD_RES`	`83 83`		`Phosphoserine; in alleles A and C.`
`MOD_RES`	`90 90`		`Phosphoserine.`
`MOD_RES`	`130 130`		`Phosphoserine.`
`VAR_SEQ`	`156 163`		`Missing (in isoform 2).`	`VSP_012408`
`VARIANT`	`28 28`	`1`	`S -> P (in allele C and allele D).`
`VARIANT`	`83 83`	`1`	`S -> N (in allele D).`
`VARIANT`	`209 209`	`1`	`I -> T.`

Sequence information

Length: 214 AA [This is the length of the unprocessed precursor]

Molecular weight: 24306 Da [This is the MW of the unprocessed precursor]

CRC64: 9312FBFFBD6E49DC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKLLILTCLV AVALARPKHP IKHQGLSSEV LNENLLRFVV APFPEVFRKE NINELSKDIG 

        70         80         90        100        110        120 
SESIEDQAME DAKQMKAGSS SSSEEIVPNS AEQKYIQKED VPSERYLGYL EQLLRLKKYN 

       130        140        150        160        170        180 
VPQLEIVPKS AEEQLHSMKE GNPAHQKQPM IAVNQELAYF YPQLFRQFYQ LDAYPSGAWY 

       190        200        210 
YLPLGTQYTD APSFSDIPNP IGSENSGKIT MPLW

P04653 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools