[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. PubMed=6319746 [NCBI, ExPASy, EBI, Israel, Japan] Herr W.; "Nucleotide sequence of AKV murine leukemia virus."; J. Virol. 49:471-478(1984).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 353-1196. DOI=10.1093/nar/10.21.6931; PubMed=6294621 [NCBI, ExPASy, EBI, Israel, Japan] Herr W., Corbin V., Gilbert W.; "Nucleotide sequence of the 3' half of AKV."; Nucleic Acids Res. 10:6931-6944(1982).

Comments

FUNCTION: During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-phosphomonoester.
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
PTM: Specific enzymatic cleavages in vivo yield mature proteins.
MISCELLANEOUS: This protein is synthesized as a Gag-Pol polyprotein.
SIMILARITY: Belongs to the retroviral Pol polyprotein family.
SIMILARITY: Contains 1 integrase catalytic domain.
SIMILARITY: Contains 1 peptidase A2 domain [view classification].
SIMILARITY: Contains 1 reverse transcriptase domain.
SIMILARITY: Contains 1 RNase H domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

J01998; AAB03091.1; ALT_INIT; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A03958; GNVWK.

3D structure databases

HSSP

P03355; 1I6J. [HSSP ENTRY / PDB]

SMR

P03356; 144-594, 623-788.

ModBase

P03356.

Protein family/group databases

MEROPS

A02.008; -.

Ontologies

GO:0004190;	Molecular function: aspartic-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0004523;	Molecular function: ribonuclease H activity (inferred from electronic annotation from InterPro).
GO:0003723;	Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003964;	Molecular function: RNA-directed DNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0015074;	Biological process: DNA integration (inferred from electronic annotation from InterPro).
GO:0006310;	Biological process: DNA recombination (inferred from electronic annotation from UniProtKB-KW).
GO:0006508;	Biological process: proteolysis (inferred from electronic annotation from InterPro).
GO:0006278;	Biological process: RNA-dependent DNA replication (inferred from electronic annotation from InterPro).
GO:0032196;	Biological process: transposition (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR000477; DNA_pol_RVTase.
IPR001584; Integrase_cat-core.
IPR001969; Pept_Asp_AS.
IPR009007; Pept_Aspartc_cat.
IPR001995; Peptidase_A2_cat.
IPR002156; RNase_H.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.70.10; Pept_Aspartc_cat; 1.

Pfam

PF00075; RnaseH; 1.
PF00665; rve; 1.
PF00077; RVP; 1.
PF00078; RVT_1; 1.
Pfam graphical view of domain structure.

PROSITE

PS50175; ASP_PROT_RETROV; 1.
PS00141; ASP_PROTEASE; 1.
PS50994; INTEGRASE; 1.
PS50879; RNASE_H; 1.
PS50878; RT_POL; 1.
PROSITE graphical view of domain structure (profiles).

Other

ProtoNet

P03356.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Aspartyl protease; DNA integration; DNA recombination; Endonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Protease; RNA-directed DNA polymerase; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1196`	`1196`	`Pol polyprotein.`	`PRO_0000259722`
`CHAIN`	`1 103`	`103`	`Protease.`	`PRO_0000026129`
`CHAIN`	`104 ?`		`Reverse transcriptase/ribonuclease H.`	`PRO_0000259723`
`CHAIN`	`? 1196`		`Integrase.`	`PRO_0000259724`
`DOMAIN`	`22 92`	`71`	`Peptidase A2.`
`DOMAIN`	`202 393`	`192`	`Reverse transcriptase.`
`DOMAIN`	`635 781`	`147`	`RNase H.`
`DOMAIN`	`905 1063`	`159`	`Integrase catalytic.`
`ACT_SITE`	`27 27`		`By similarity.`
`CONFLICT`	`540 540`		`R -> T (in Ref. 2).`
`CONFLICT`	`996 996`		`Missing (in Ref. 2).`

Sequence information

Length: 1196 AA [This is the length of the unprocessed precursor]

Molecular weight: 133413 Da [This is the MW of the unprocessed precursor]

CRC64: EDBAE9F550839A01 [This is a checksum on the sequence]

        10         20         30         40         50         60 
GGQGQEPPPE PRITLTVGGQ PVTFLVDTGA QHSVLTQNPG PLSDRSAWVQ GATGGKRYRW 

        70         80         90        100        110        120 
TTDRKVHLAT GKVTHSFLHV PDCPYPLLGR DLLTKLKAQI HFEGSGAQVV GPKGQPLQVL 

       130        140        150        160        170        180 
TLNLEDEYRL YETSAEPEVS PGSTWLSDFP QAWAETGGMG LAVRQAPLII PLKATSTPVS 

       190        200        210        220        230        240 
IKQYPMSQEA KLGIKPHIQR LLDQGILVPC QSPWNTPLLP VKKPGTNDYR PVQDLREVNK 

       250        260        270        280        290        300 
RVEDIHPTVP NPYNLLSGLP PSHRWYTVLD LKDAFFCLRL HPTSQPLFAF EWRDPGMGIS 

       310        320        330        340        350        360 
GQLTWTRLPQ GFKNSPTLFD EALHRDLADF RIQHPDLILL QYVDDILLAA TSELDCQQGT 

       370        380        390        400        410        420 
RALLLTLGNL GYRASAKKAQ LCQKQVKYLG YLLKEGQRWL TEARKETVMG QPTPKTPRQL 

       430        440        450        460        470        480 
REFLGTAGFC RLWIPGFAEM AAPLYPLTKT GTLFNWGPDQ QKAYQEIKQA LLTAPALGLP 

       490        500        510        520        530        540 
DLTKPFELFV DEKQGYAKGV LTQKLGPWRR PVAYLSKKLD PVAAGWPPCL RMVAAIAVLR 

       550        560        570        580        590        600 
KDAGKLTMGQ PLVILAPHAV EALVKQPPDR WLSNARMTHY QAMLLDTDRV QFGPVVALNP 

       610        620        630        640        650        660 
ATLLPLPEEG APHDCLEILA ETHGTRPDLT DQPIPDADHT WYTDGSSFLQ EGQRKAGAAV 

       670        680        690        700        710        720 
TTETEVIWAR ALPAGTSAQR AELIALTQAL KMAEGKRLNV YTDSRYAFAT AHIHGEIYRR 

       730        740        750        760        770        780 
RGLLTSEGRE IKNKSEILAL LKALFLPKRL SIIHCLGHQK GDSAEARGNR LADQAAREAA 

       790        800        810        820        830        840 
IKTPPDTSTL LIEDSTPYTP AYFHYTETDL KKLRELGATY NQSKGYWVFQ GKPVMPDQFV 

       850        860        870        880        890        900 
FELLDSLHRL THLGYQKMKA LLDRGESPYY MLNRDKTLQY VADSCTVCAQ VNASKAKIGA 

       910        920        930        940        950        960 
GVRVRGHRPG SHWEIDFTEV KPGLYGYKYL LVFVDTFSGW VEAFPTKRET ARVVSKKLLE 

       970        980        990       1000       1010       1020 
EIFPRFGMPQ VLGSDNGPAF TSQVSQSVAD LLGIDWKLHC AYRPQSSGQV ERMNRTIKET 

      1030       1040       1050       1060       1070       1080 
LTKLTLAAGT RDWVLLLPLA LYRARNTPGP HGLTPYEILY GAPPPLVNFH DPDMSELTNS 

      1090       1100       1110       1120       1130       1140 
PSLQAHLQAL QTVQREIWKP LAEAYRDQLD QPVIPHPFRI GDSVWVRRHQ TKNLEPRWKG 

      1150       1160       1170       1180       1190 
PYTVLLTTPT ALKVDGISAW IHAAHVKAAT TPPIKPSWRV QRSQNPLKIR LTRGAP

P03356 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools