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UniProtKB/Swiss-Prot entry O82663


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHSA1_ARATH
Primary accession number O82663
Secondary accession numbers None
Integrated into Swiss-Prot on May 2, 2002
Sequence was last modified on November 1, 1998 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 81)
Name and origin of the protein
Protein name Succinate dehydrogenase [ubiquinone] flavoprotein subunit 1, mitochondrial [Precursor]
Synonyms EC 1.3.5.1
Flavoprotein subunit 1 of complex II
FP
Gene name
Name: SDH1-1
OrderedLocusNames: At5g66760
ORFNames: MSN2.16
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. C24;
Machuy N., Klein M., Mueller-Roeber B.;
"Cloning and characterization of succinyl-CoA-ligase from Arabidopsis thaliana.";
Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/7.1.31; PubMed=10718197 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones.";
DNA Res. 7:31-63(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[4]
PROTEIN SEQUENCE OF 160-170.
TISSUE=Leaf, and Stem;
DOI=10.1104/pp.127.4.1694; PubMed=11743114 [NCBI, ExPASy, EBI, Israel, Japan]
Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.;
"Proteomic approach to identify novel mitochondrial proteins in Arabidopsis.";
Plant Physiol. 127:1694-1710(2001).
[5]
SUBUNIT, AND TISSUE SPECIFICITY.
DOI=10.1023/A:1019926301981; PubMed=12374303 [NCBI, ExPASy, EBI, Israel, Japan]
Figueroa P., Leon G., Elorza A., Holuigue L., Araya A., Jordana X.;
"The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes and targeted to mitochondria in Arabidopsis thaliana.";
Plant Mol. Biol. 50:725-734(2002).
[6]
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1105/tpc.016055; PubMed=14671022 [NCBI, ExPASy, EBI, Israel, Japan]
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.;
"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins.";
Plant Cell 16:241-256(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AJ001809; CAA05025.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB018119; BAA97282.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF367341; AAK32928.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY045674; AAK74032.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF436833; AAL32015.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY124812; AAM70521.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T51815; T51815.
RefSeq NP_201477.1; -.
UniGene At.22655
3D structure databases
HSSP P10444; 1NEK. [HSSP ENTRY / PDB]
SMR O82663; 41-634.
ModBase O82663.
Enzyme and pathway databases
BioCyc MetaCyc:AT5G66760-MON; -.
Organism-specific databases
GeneFarm 2175; 174.
TAIR At5g66760; -.
Gene expression databases
ArrayExpress O82663; -.
GermOnline AT5G66760; Arabidopsis thaliana.
Ontologies
GO
GO:0005618; Cellular component: cell wall (inferred from direct assay from TAIR).
GO:0005743; Cellular component: mitochondrial inner membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from direct assay from TAIR).
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0050660; Molecular function: FAD binding (inferred from electronic annotation from InterPro).
GO:0008177; Molecular function: succinate dehydrogenase (ubiquinone) activity (inferred from electronic annotation from EC).
GO:0000104; Molecular function: succinate dehydrogenase activity (traceable author statement from TAIR).
GO:0006121; Biological process: mitochondrial electron transport, succinate to ubiquinone (traceable author statement from TAIR).
GO:0006810; Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
GO:0006099; Biological process: tricarboxylic acid cycle (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR003953; FAD_bind2_N.
IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR003952; FRD_SDH_FAD_BS.
IPR004112; Fum_Rdtase/Succ_DHase_flav_C.
IPR011281; Succ_DHase_flav_su_fwd.
IPR014006; Succ_Dhase_frdA_Gneg.
Graphical view of domain structure.
Pfam PF00890; FAD_binding_2; 1.
PF02910; Succ_DH_flav_C; 1.
Pfam graphical view of domain structure.
PRINTS PR00368; FADPNR.
TIGRFAMs TIGR01816; sdhA_forward; 1.
TIGR01812; sdhA_frdA_Gneg; 1.
PROSITE PS00504; FRD_SDH_FAD_BINDING; 1.
Proteomics databases
PRIDE O82663; -.
Genome annotation databases
GeneID 836809; -.
GenomeReviews BA000015_GR; AT5G66760.
KEGG ath:AT5G66760; -.
NMPDR fig|3702.1.peg.28782; -.
Other
ProtoNet O82663.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; Electron transport; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Transit peptide; Transport; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    32  32     Mitochondrion (Potential). 
CHAIN   33   634  602     Succinate dehydrogenase [ubiquinone] flavoprotein subunit 1, mitochondrial. PRO_0000158659
NP_BIND   79    94  16     FAD (By similarity). 
ACT_SITE   329   329        Proton acceptor (By similarity). 
BINDING   285   285        Substrate (By similarity). 
BINDING   297   297        Substrate (By similarity). 
BINDING   396   396        Substrate (By similarity). 
BINDING   441   441        Substrate (By similarity). 
MOD_RES   87    87        Tele-8alpha-FAD histidine (By similarity). 
Sequence information
Length: 634 AA [This is the length of the unprocessed precursor] Molecular weight: 69656 Da [This is the MW of the unprocessed precursor] CRC64: AECE471C7AD43B84 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MWRCVSRGFR APASKTSSLF DGVSGSRFSR FFSTGSTDTR SSYTIVDHTY DAVVVGAGGA 

        70         80         90        100        110        120 
GLRAAIGLSE HGFNTACITK LFPTRSHTVA AQGGINAALG NMSEDDWRWH MYDTVKGSDW 

       130        140        150        160        170        180 
LGDQDAIQYM CREAPKAVIE LENYGLPFSR TEEGKIYQRA FGGQSLDFGK GGQAYRCACA 

       190        200        210        220        230        240 
ADRTGHALLH TLYGQAMKHN TQFFVEYFAL DLLMASDGSC QGVIALNMED GTLHRFRSSQ 

       250        260        270        280        290        300 
TILATGGYGR AYFSATSAHT CTGDGNAMVA RAGLPLQDLE FVQFHPTGIY GAGCLITEGS 

       310        320        330        340        350        360 
RGEGGILRNS EGERFMERYA PTAKDLASRD VVSRSMTMEI REGRGVGPHK DHIYLHLNHL 

       370        380        390        400        410        420 
PPEVLKERLP GISETAAIFA GVDVTKEPIP VLPTVHYNMG GIPTNYHGEV VTIKGDDPDA 

       430        440        450        460        470        480 
VIPGLMAAGE AACASVHGAN RLGANSLLDI VVFGRACANR VAEISKPGEK QKPLEKDAGE 

       490        500        510        520        530        540 
KTIAWLDRLR NSNGSLPTST IRLNMQRIMQ NNAAVFRTQE TLEEGCQLID KAWESFGDVQ 

       550        560        570        580        590        600 
VKDRSMIWNS DLIETLELEN LLINASITMH SAEARKESRG AHAREDFTKR EDGEWMKHTL 

       610        620        630 
GYWEDEKVRL DYRPVHMDTL DDEIDTFPPK ARVY 

O82663 in FASTA format

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View entry in raw text format (no links)
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