[1]	NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND DEVELOPMENTAL STAGE. STRAIN=cv. ILC 3279; DOI=10.1016/S0168-9452(00)00214-4; PubMed=10773344 [NCBI, ExPASy, EBI, Israel, Japan] Overkamp S., Hein F., Barz W.; "Cloning and characterization of eight cytochrome P450 cDNAs from chickpea (Cicer arietinum L.) cell suspension cultures."; Plant Sci. 155:101-108(2000).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=cv. ILC 3279; Overkamp S., Barz W.; "Isolation of a full length cDNA encoding trans-cinnamate 4-hydroxylase from chickpea (Cicer arietinum L.)."; (er) Plant Gene Register PGR99-086.

Comments

FUNCTION: Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.
CATALYTIC ACTIVITY: Trans-cinnamate + NADPH + O₂ = 4-hydroxycinnamate + NADP⁺ + H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Phenylpropanoid metabolism; 4-coumaric acid biosynthesis; 4-coumaric acid from trans-cinnamic acid: step 1/1 .
DEVELOPMENTAL STAGE: Detectable 2 hours after elicitation and disappears after 6 hours.
INDUCTION: By elicitation.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ007449; CAA07519.2; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T09525; T09525.

3D structure databases

ModBase

O81928.

Ontologies

GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0020037;	Molecular function: heme binding (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0016710;	Molecular function: trans-cinnamate 4-monooxygenase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

Other

ProtoNet

O81928.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 505`	`505`	`Trans-cinnamate 4-monooxygenase.`	`PRO_0000052244`
`METAL`	`446 446`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 505 AA [This is the length of the unprocessed precursor]

Molecular weight: 57889 Da [This is the MW of the unprocessed precursor]

CRC64: 453D1858FA996DC0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDLLLLEKTL LALFIAATIA ITISKLRGKR FKLPPGPIPV PVFGNWLQVG DDLNHRNLTD 

        70         80         90        100        110        120 
LAKRFGDIFL LRMGQRNLVV VSSPELAKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT 

       130        140        150        160        170        180 
VYGTLAEMRR IMTVPFFTNK VVQQYRFGWE FEAQSVVDDV KKNPEACSSG IVLRRRLQLM 

       190        200        210        220        230        240 
MYNIMYRIMF DRRFESEEDP LFVKLKALNG ERSRLAQSFE YNYGDFIPIL RPFLKGYLKL 

       250        260        270        280        290        300 
CKEVKDRRLQ LFKDYFVDER KKLGSTKSTT NEGLKCAIDH ILDAQQKGEI NDDNVLYIVE 

       310        320        330        340        350        360 
NINVAAIETT LWSIEWGIAE LVNHQKIQNK VREEIDRVLG PGHQVTEPDL QKLPYLQAVI 

       370        380        390        400        410        420 
KETLRLRMAI PLLVPHMNLH DAKLSGFDIP AESKILVNAW WLANNPAQWK KPEEFRPERF 

       430        440        450        460        470        480 
LEEESHVEAN GNDFRYLPFG VGRRSCPGII LALPILGITL GRLVQNFELL PPPGQSKIDT 

       490        500 
AEKGGQFSLH ILKHSTIVCK PRSFN

O81928 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools