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UniProtKB/Swiss-Prot entry Q8L5W8


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PIL1_ARATH
Primary accession number Q8L5W8
Secondary accession numbers O80727 Q84WY4
Integrated into Swiss-Prot on December 16, 2008
Sequence was last modified on October 1, 2002 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 36)
Name and origin of the protein
Protein name Transcription factor PIL1
Synonyms Protein PHYTOCHROME INTERACTING FACTOR 3-LIKE 1
Transcription factor EN 110
bHLH transcription factor bHLH124
Basic helix-loop-helix protein 124
bHLH 124
AtbHLH124
Gene name
Name: PIL1
Synonyms: BHLH124, EN110
OrderedLocusNames: At2g46970
ORFNames: F14M4.20
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH APRR1/TOC1.
DOI=10.1093/pcp/pcf005; PubMed=11828023 [NCBI, ExPASy, EBI, Israel, Japan]
Makino S., Matsushika A., Kojima M., Yamashino T., Mizuno T.;
"The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana: I. Characterization with APRR1-overexpressing plants.";
Plant Cell Physiol. 43:58-69(2002).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/45471; PubMed=10617197 [NCBI, ExPASy, EBI, Israel, Japan]
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
Nature 402:761-768(1999).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1104/pp.010207; PubMed=12481096 [NCBI, ExPASy, EBI, Israel, Japan]
Xiao Y.-L., Malik M., Whitelaw C.A., Town C.D.;
"Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2 of Arabidopsis.";
Plant Physiol. 130:2118-2128(2002).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
Underwood B.A., Xiao Y., Moskal W., Monaghan E., Wang W., Redman J., Wu H.C., Utterback T., Town C.D.;
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
[5]
GENE FAMILY, AND NOMENCLATURE.
DOI=10.1093/molbev/msg088; PubMed=12679534 [NCBI, ExPASy, EBI, Israel, Japan]
Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
"The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.";
Mol. Biol. Evol. 20:735-747(2003).
[6]
FUNCTION, AND INDUCTION.
DOI=10.1038/nature02174; PubMed=14668869 [NCBI, ExPASy, EBI, Israel, Japan]
Salter M.G., Franklin K.A., Whitelam G.C.;
"Gating of the rapid shade-avoidance response by the circadian clock in plants.";
Nature 426:680-683(2003).
[7]
GENE FAMILY.
DOI=10.1105/tpc.013839; PubMed=12897250 [NCBI, ExPASy, EBI, Israel, Japan]
Toledo-Ortiz G., Huq E., Quail P.H.;
"The Arabidopsis basic/helix-loop-helix transcription factor family.";
Plant Cell 15:1749-1770(2003).
[8]
INTERACTION WITH APRR1/TOC1, AND TISSUE SPECIFICITY.
DOI=10.1093/pcp/pcg078; PubMed=12826627 [NCBI, ExPASy, EBI, Israel, Japan]
Yamashino T., Matsushika A., Fujimori T., Sato S., Kato T., Tabata S., Mizuno T.;
"A Link between circadian-controlled bHLH factors and the APRR1/TOC1 quintet in Arabidopsis thaliana.";
Plant Cell Physiol. 44:619-629(2003).
[9]
FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
DOI=10.1105/tpc.106.042200; PubMed=16891401 [NCBI, ExPASy, EBI, Israel, Japan]
Khanna R., Shen Y., Toledo-Ortiz G., Kikis E.A., Johannesson H., Hwang Y.-S., Quail P.H.;
"Functional profiling reveals that only a small number of phytochrome-regulated early-response genes in Arabidopsis are necessary for optimal deetiolation.";
Plant Cell 18:2157-2171(2006).
[10]
FUNCTION, AND INDUCTION BY FAR-RED LIGHT.
DOI=10.1104/pp.105.076331; PubMed=16565297 [NCBI, ExPASy, EBI, Israel, Japan]
Roig-Villanova I., Bou J., Sorin C., Devlin P.F., Martinez-Garcia J.F.;
"Identification of primary target genes of phytochrome signaling. Early transcriptional control during shade avoidance responses in Arabidopsis.";
Plant Physiol. 141:85-96(2006).
Comments
  • FUNCTION: Transcription factor. Involved in responses to transient and long-term shade. Required for the light-mediated inhibition of hypocotyl elongation. Necessary for rapid light-induced expression of the photomorphogenesis- and circadian-related gene APRR9. Seems to play a role in multiple PHYB responses, such as flowering transition and petiole elongation.
  • SUBUNIT: Homodimer (Probable). Interacts with APRR1/TOC1.
  • SUBCELLULAR LOCATION: Nucleus.
  • TISSUE SPECIFICITY: Etiolated seedlings.
  • INDUCTION: Expressed with a circadian rhythm showing peaks during the light period. Up-regulated by simulated shade in light-grown plants, in a phytochrome-dependent manner; low red/far-red ratio (R/FR) light, but repressed by a high R/FR light. Rapidly down-regulated after seedling deetiolation.
  • SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain.
  • SEQUENCE CAUTION:
    • Sequence=AAC34226.1; Type=Erroneous gene model prediction;
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB090873; BAC10689.1; -; pre-RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC004411; AAC34226.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY219127; AAO37214.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY954840; AAX55166.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T02190; T02190.
RefSeq NP_182220.2; -.
UniGene At.36425
3D structure databases
ModBase Q8L5W8.
Organism-specific databases
TAIR At2g46970; -.
Family and domain databases
InterPro IPR001092; HLH_basic.
IPR011598; HLH_DNA_bd.
Graphical view of domain structure.
Gene3D G3DSA:4.10.280.10; HLH_DNA_bd; 1.
Pfam PF00010; HLH; 1.
Pfam graphical view of domain structure.
SMART SM00353; HLH; 1.
SMART graphical view of domain structure.
PROSITE PS50888; HLH; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
GeneID 819311; -.
KEGG ath:AT2G46970; -.
NMPDR fig|3702.1.peg.11885; -.
Other
ProtoNet Q8L5W8.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Coiled coil; Complete proteome; DNA-binding; Nucleus; Transcription; Transcription regulation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   416  416     Transcription factor PIL1. PRO_0000358853
DOMAIN   243   283  41     Helix-loop-helix motif. 
DNA_BIND   219   242  24     Basic motif (By similarity). 
COILED   95   124  30     Potential. 
CONFLICT   302   302        H -> K (in Ref. 3; AAO37214). 
Sequence information
Length: 416 AA [This is the length of the unprocessed precursor] Molecular weight: 46580 Da [This is the MW of the unprocessed precursor] CRC64: 69B86687CC1901CF [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEAKPLASSS SEPNMISPSS NIKPKLKDED YMELVCENGQ ILAKIRRPKN NGSFQKQRRQ 

        70         80         90        100        110        120 
SLLDLYETEY SEGFKKNIKI LGDTQVVPVS QSKPQQDKET NEQMNNNKKK LKSSKIEFER 

       130        140        150        160        170        180 
NVSKSNKCVE SSTLIDVSAK GPKNVEVTTA PPDEQSAAVG RSTELYFASS SKFSRGTSRD 

       190        200        210        220        230        240 
LSCCSLKRKY GDIEEEESTY LSNNSDDESD DAKTQVHART RKPVTKRKRS TEVHKLYERK 

       250        260        270        280        290        300 
RRDEFNKKMR ALQDLLPNCY KDDKASLLDE AIKYMRTLQL QVQMMSMGNG LIRPPTMLPM 

       310        320        330        340        350        360 
GHYSPMGLGM HMGAAATPTS IPQFLPMNVQ ATGFPGMNNA PPQMLSFLNH PSGLIPNTPI 

       370        380        390        400        410 
FSPLENCSQP FVVPSCVSQT QATSFTQFPK SASASNLEDA MQYRGSNGFS YYRSPN 

Q8L5W8 in FASTA format

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