ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry O80536


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name PIF3_ARATH
Primary accession number O80536
Secondary accession numbers A5Y7A2 A5Y7A3 A5Y7A4 A5Y7A5 A5Y7A6 A5Y7A7 A5Y7A8 A5Y7A9 A5Y7B0 A5Y7B1 A5Y7B2 A5Y7B3 A5Y7B4 A5Y7B5 A5Y7B6 Q9SBC5
Integrated into Swiss-Prot on May 10, 2002
Sequence was last modified on November 1, 1998 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 71)
Name and origin of the protein
Protein name Transcription factor PIF3
Synonyms Phytochrome-interacting factor 3
Phytochrome-associated protein 3
Transcription factor EN 100
bHLH transcription factor bHLH008
Basic helix-loop-helix protein 8
bHLH 8
AtbHLH8
Gene name
Name: PIF3
Synonyms: BHLH8, EN100, PAP3
OrderedLocusNames: At1g09530
ORFNames: F14J9.19
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
STRAIN=cv. Columbia;
DOI=10.1016/S0092-8674(00)81636-0; PubMed=9845368 [NCBI, ExPASy, EBI, Israel, Japan]
Ni M., Tepperman J.M., Quail P.H.;
"PIF3, a phytochrome-interacting factor necessary for normal photoinduced signal transduction, is a novel basic helix-loop-helix protein.";
Cell 95:657-667(1998).
[2]
NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY UV LIGHT, GENE FAMILY, AND NOMENCLATURE.
STRAIN=cv. Columbia;
DOI=10.1093/molbev/msg088; PubMed=12679534 [NCBI, ExPASy, EBI, Israel, Japan]
Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
"The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.";
Mol. Biol. Evol. 20:735-747(2003).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Columbia;
Lee J., Yi H., Shin B., Song P.-S., Choi G.;
"Identification and characterization of three phytochrome-associated proteins.";
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048500; PubMed=11130712 [NCBI, ExPASy, EBI, Israel, Japan]
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
Nature 408:816-820(2000).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1071006; PubMed=11910074 [NCBI, ExPASy, EBI, Israel, Japan]
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.;
"Functional annotation of a full-length Arabidopsis cDNA collection.";
Science 296:141-145(2002).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-272, AND VARIANTS ASP-124; ASN-141; HIS-176; LYS-186; LEU-209; ILE-220 AND ILE-236.
STRAIN=cv. An-2, cv. Bla-6, cv. Br-0, cv. Bu-2, cv. Columbia, cv. Di-1, cv. Et-0, cv. Kl-1, cv. Li-5:3, cv. Ma-2, cv. Mt-0, cv. Pa-2, cv. Pi-0, cv. Su-0, and cv. Tsu-1;
DOI=10.1111/j.1365-294X.2007.03298.x; PubMed=17614917 [NCBI, ExPASy, EBI, Israel, Japan]
Brock M.T., Tiffin P., Weinig C.;
"Sequence diversity and haplotype associations with phenotypic responses to crowding: GIGANTEA affects fruit set in Arabidopsis thaliana.";
Mol. Ecol. 16:3050-3062(2007).
[7]
FUNCTION.
DOI=10.1038/23500; PubMed=10466729 [NCBI, ExPASy, EBI, Israel, Japan]
Ni M., Tepperman J.M., Quail P.H.;
"Binding of phytochrome B to its nuclear signalling partner PIF3 is reversibly induced by light.";
Nature 400:781-784(1999).
[8]
FUNCTION.
DOI=10.1126/science.288.5467.859; PubMed=10797009 [NCBI, ExPASy, EBI, Israel, Japan]
Martinez-Garcia J.F., Huq E., Quail P.H.;
"Direct targeting of light signals to a promoter element-bound transcription factor.";
Science 288:859-863(2000).
[9]
INTERACTION WITH APRR1.
DOI=10.1093/pcp/pcf005; PubMed=11828023 [NCBI, ExPASy, EBI, Israel, Japan]
Makino S., Matsushika A., Kojima M., Yamashino T., Mizuno T.;
"The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana: I. Characterization with APRR1-overexpressing plants.";
Plant Cell Physiol. 43:58-69(2002).
[10]
GENE FAMILY, AND INTERACTION WITH PIF4.
DOI=10.1105/tpc.013839; PubMed=12897250 [NCBI, ExPASy, EBI, Israel, Japan]
Toledo-Ortiz G., Huq E., Quail P.H.;
"The Arabidopsis basic/helix-loop-helix transcription factor family.";
Plant Cell 15:1749-1770(2003).
[11]
INTERACTION WITH APRR1.
DOI=10.1093/pcp/pcg078; PubMed=12826627 [NCBI, ExPASy, EBI, Israel, Japan]
Yamashino T., Matsushika A., Fujimori T., Sato S., Kato T., Tabata S., Mizuno T.;
"A Link between circadian-controlled bHLH factors and the APRR1/TOC1 quintet in Arabidopsis thaliana.";
Plant Cell Physiol. 44:619-629(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF100166; AAC95156.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF251693; AAL55715.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF088280; AAC99771.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC003970; AAC33213.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK117255; BAC41930.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193482; ABP96435.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193483; ABP96436.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193484; ABP96437.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193485; ABP96438.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193486; ABP96439.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193487; ABP96440.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193488; ABP96441.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193489; ABP96442.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193490; ABP96443.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193491; ABP96444.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193492; ABP96445.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193493; ABP96446.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193494; ABP96447.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193495; ABP96448.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF193496; ABP96449.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR H86228; H86228.
RefSeq NP_172424.1; -.
NP_849626.1; -.
UniGene At.10926
3D structure databases
HSSP P25912; 1HLO. [HSSP ENTRY / PDB]
ModBase O80536.
Protein-protein interaction databases
IntAct O80536; 12.
Organism-specific databases
TAIR At1g09530; -.
Gene expression databases
ArrayExpress O80536; -.
GermOnline AT1G09530; Arabidopsis thaliana.
Ontologies
GO
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from InterPro).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from UniProtKB-KW).
GO:0042802; Molecular function: identical protein binding (inferred from physical interaction from IntAct).
GO:0030528; Molecular function: transcription regulator activity (inferred from electronic annotation from InterPro).
GO:0009704; Biological process: de-etiolation (inferred from mutant phenotype from TAIR).
GO:0009740; Biological process: gibberellic acid mediated signaling (inferred from mutant phenotype from TAIR).
GO:0031539; Biological process: positive regulation of anthocyanin metabolic process (inferred from mutant phenotype from TAIR).
GO:0010017; Biological process: red or far red light signaling pathway (inferred from electronic annotation from UniProtKB-KW).
GO:0009585; Biological process: red, far-red light phototransduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006355; Biological process: regulation of transcription, DNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001092; HLH_basic.
IPR011598; HLH_DNA_bd.
Graphical view of domain structure.
Gene3D G3DSA:4.10.280.10; HLH_DNA_bd; 1.
Pfam PF00010; HLH; 1.
Pfam graphical view of domain structure.
SMART SM00353; HLH; 1.
SMART graphical view of domain structure.
PROSITE PS50888; HLH; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
GeneID 837479; -.
GenomeReviews CT485782_GR; AT1G09530.
KEGG ath:AT1G09530; -.
NMPDR fig|3702.1.peg.1178; -.
Other
ProtoNet O80536.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; DNA-binding; Nucleus; Phytochrome signaling pathway; Transcription; Transcription regulation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   524  524     Transcription factor PIF3. PRO_0000127428
DOMAIN   357   397  41     Helix-loop-helix motif. 
DNA_BIND   340   356  17     Basic motif. 
COMPBIAS   353   358  6     Poly-Arg. 
COMPBIAS   422   428  7     Poly-Ala. 
VARIANT   124   124  1     E -> D (in strain: cv. An-2 and cv. Su-0). 
VARIANT   141   141  1     K -> N (in strain: cv. An-2 and cv. Su-0). 
VARIANT   176   176  1     Q -> H (in strain: cv. An-2 and cv. Su-0). 
VARIANT   186   186  1     Q -> K (in strain: cv. An-2 and cv. Su-0). 
VARIANT   209   209  1     F -> L (in strain: cv. An-2 and cv. Su-0). 
VARIANT   220   220  1     T -> I (in strain: cv. An-2 and cv. Su-0). 
VARIANT   236   236  1     V -> I (in strain: cv. An-2 and cv. Su-0). 
CONFLICT   15    15        E -> D (in Ref. 3; AAC99771). 
CONFLICT   344   344        S -> L (in Ref. 3; AAC99771). 
Sequence information
Length: 524 AA [This is the length of the unprocessed precursor] Molecular weight: 56990 Da [This is the MW of the unprocessed precursor] CRC64: 1044AC01D598DE7C [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPLFELFRLT KAKLESAQDR NPSPPVDEVV ELVWENGQIS TQSQSSRSRN IPPPQANSSR 

        70         80         90        100        110        120 
AREIGNGSKT TMVDEIPMSV PSLMTGLSQD DDFVPWLNHH PSLDGYCSDF LRDVSSPVTV 

       130        140        150        160        170        180 
NEQESDMAVN QTAFPLFQRR KDGNESAPAA SSSQYNGFQS HSLYGSDRAR DLPSQQTNPD 

       190        200        210        220        230        240 
RFTQTQEPLI TSNKPSLVNF SHFLRPATFA KTTNNNLHDT KEKSPQSPPN VFQTRVLGAK 

       250        260        270        280        290        300 
DSEDKVLNES VASATPKDNQ KACLISEDSC RKDQESEKAV VCSSVGSGNS LDGPSESPSL 

       310        320        330        340        350        360 
SLKRKHSNIQ DIDCHSEDVE EESGDGRKEA GPSRTGLGSK RSRSAEVHNL SERRRRDRIN 

       370        380        390        400        410        420 
EKMRALQELI PNCNKVDKAS MLDEAIEYLK SLQLQVQIMS MASGYYLPPA VMFPPGMGHY 

       430        440        450        460        470        480 
PAAAAAMAMG MGMPYAMGLP DLSRGGSSVN HGPQFQVSGM QQQPVAMGIP RVSGGGIFAG 

       490        500        510        520 
SSTIGNGSTR DLSGSKDQTT TNNNSNLKPI KRKQGSSDQF CGSS 

O80536 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by kr flag YPRC Korea Mirror sites: Australia  Brazil  Canada  China  Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!