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UniProtKB/Swiss-Prot entry O74557


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NCB5R_SCHPO
Primary accession number O74557
Secondary accession numbers None
Integrated into Swiss-Prot on November 13, 2007
Sequence was last modified on November 1, 1998 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 40)
Name and origin of the protein
Protein name NADH-cytochrome b5 reductase 1
Synonyms EC 1.6.2.2
Microsomal cytochrome b reductase
Gene name
Name: cbr1
ORFNames: SPCC970.03
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
[2]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1038/nbt1222; PubMed=16823372 [NCBI, ExPASy, EBI, Israel, Japan]
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.;
"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.";
Nat. Biotechnol. 24:841-847(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
CU329672; CAA20696.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T41677; T41677.
RefSeq NP_587852.1; -.
3D structure databases
HSSP P20070; 1I7P. [HSSP ENTRY / PDB]
ModBase O74557.
Organism-specific databases
GeneDB_Spombe SPCC970.03; -.
Gene expression databases
ArrayExpress O74557; -.
Ontologies
GO
GO:0005789; Cellular component: endoplasmic reticulum membrane (inferred by curator from GeneDB_SPombe).
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005741; Cellular component: mitochondrial outer membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0004128; Molecular function: cytochrome-b5 reductase activity (inferred from electronic annotation from EC).
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0016192; Biological process: vesicle-mediated transport (inferred by curator from GeneDB_SPombe).
QuickGo view.
Family and domain databases
InterPro IPR001834; Cyt_B5_reductase.
IPR001709; FPN_cyt_redctse.
IPR008333; OxRdtase_FAD-bd.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.
Pfam PF00970; FAD_binding_6; 1.
PF00175; NAD_binding_1; 1.
Pfam graphical view of domain structure.
PRINTS PR00406; CYTB5RDTASE.
PR00371; FPNCR.
PROSITE PS51384; FAD_FR; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
GeneID 2539526; -.
KEGG spo:SPCC970.03; -.
NMPDR fig|4896.1.peg.190; -.
Other
ProtoNet O74557.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Endoplasmic reticulum; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion outer membrane; NAD; Oxidoreductase; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   301  301     NADH-cytochrome b5 reductase 1. PRO_0000310844
TRANSMEM   8    28  21     Potential. 
TRANSMEM   32    49  18     Potential. 
DOMAIN   59   162  104     FAD-binding FR-type. 
NP_BIND   142   157  16     FAD (By similarity). 
NP_BIND   168   199  32     FAD (By similarity). 
Sequence information
Length: 301 AA [This is the length of the unprocessed precursor] Molecular weight: 33723 Da [This is the MW of the unprocessed precursor] CRC64: 455412A9AB47AA6B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAKQTLLSTP LHGVYIPVFL IIFGTYIVKR EWVGYAIVVA FSLGFHKFWR GRVRKVLSDK 

        70         80         90        100        110        120 
IQQFELSDKA VLNHNTAIYR FRLPRANDVL GLPIGQHLKV FVDVDGKEYS RSYTPLSSDA 

       130        140        150        160        170        180 
DKGYFDLLVK SYPNGKVSKK FSELKIGDTI GVRGPKGNWK HRTGLARHFG MIAGGTGITP 

       190        200        210        220        230        240 
MLQIIRAVLS NFEDPTEITL LYANVSEGDI VLRDEIDALA KKDPRFTVHY VLNNPPENWK 

       250        260        270        280        290        300 
GSVGFVTQEL IKAHFPAPSP ETKVLICGPT PMVNSLREAT VALGYEKSRA ISKLEDQVFV 


F 

O74557 in FASTA format

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