NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=VF5;
DOI=10.1038/32831; PubMed=9537320 [NCBI, ExPASy, EBI, Israel, Japan]
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
Nature 392:353-358(1998).

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE000657; AAC07039.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

F70380; F70380.

RefSeq

NP_213635.1; -.

3D structure databases

ModBase

O67073.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_931-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0003934;	Molecular function: GTP cyclohydrolase I activity (inferred from electronic annotation from InterPro).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0033739;	Molecular function: queuine synthase activity (inferred from electronic annotation from EC).
GO:0019438;	Biological process: aromatic compound biosynthetic process (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00818; -; 1.
PBIL [Tree]

InterPro

IPR016856; CN_OxRdtase_NADPH-dep_QueF.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF027377; Nitrile_oxidored_QueF; 1.

ProDom

PD003330; GTP_cyclohydroI; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR03139; QueF-II; 1.

Genome annotation databases

1193615; -.

AE000657_GR; aq_931.

aae:aq_931; -.

fig|224324.1.peg.650; -.

Phylogenomic databases

HOGENOM

O67073; -.

Other

LinkHub

O67073; -.

Genome annotation databases

CMR

O67073; aq_931.

Other

ProtoNet

O67073.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 129`	`129`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_0000162949`

Sequence information

Length: 129 AA [This is the length of the unprocessed precursor]

Molecular weight: 15331 Da [This is the MW of the unprocessed precursor]

CRC64: 3B8E508D83A3B3B2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEAKEKKYGE IEIEKAQLEA WPNPNPERDY MIEITFPEFT CLCPRSGYPD FATIKIRYIP 

        70         80         90        100        110        120 
DKYIVELKSL KLWLNKFRNR YISHEAATNE IYQALYDLLK PRFLEVVGDF HPRGNVHTVV 


RVRSDENYG

O67073 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools