[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=cv. Acala SJ2; Loguercio L.L., Wilkins T.A.; "Two genomic clones encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase from cotton (Gossypium hirsutum L.)."; (er) Plant Gene Register PGR98-031.

Comments

FUNCTION: Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP⁺ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3 .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Plastid membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the HMG-CoA reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF038046; AAC05089.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T09785; T09785.

3D structure databases

HSSP

P04035; 1HWI. [HSSP ENTRY / PDB]

ModBase

O64967.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0031966;	Cellular component: mitochondrial membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0042170;	Cellular component: plastid membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0004420;	Molecular function: hydroxymethylglutaryl-CoA reductase (NADPH) activity (inferred from electronic annotation from InterPro).
GO:0050661;	Molecular function: NADP binding (inferred from electronic annotation from InterPro).
GO:0015936;	Biological process: coenzyme A metabolic process (inferred from electronic annotation from InterPro).
GO:0008299;	Biological process: isoprenoid biosynthetic process (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR002202; HMG_CoA_Rdtase_cat.
IPR004554; HMG_CoA_Rdtase_I_cat.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.770.10; HMG-CoA_red; 1.

PANTHER

PTHR10572; HMG-CoA_red; 1.

Pfam

PF00368; HMG-CoA_red; 1.
Pfam graphical view of domain structure.

PRINTS

PR00071; HMGCOARDTASE.

TIGRFAMs

TIGR00533; HMG_CoA_R_NADP; 1.

PROSITE

PS00066; HMG_COA_REDUCTASE_1; 1.
PS00318; HMG_COA_REDUCTASE_2; 1.
PS01192; HMG_COA_REDUCTASE_3; 1.
PS50065; HMG_COA_REDUCTASE_4; 1.
PROSITE graphical view of domain structure (profiles).

Other

ProtoNet

O64967.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Glycoprotein; Isoprene biosynthesis; Membrane; Mitochondrion; NADP; Oxidoreductase; Plastid; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 628`	`628`	`3-hydroxy-3-methylglutaryl-coenzyme A reductase 2.`	`PRO_0000114439`
`TRANSMEM`	`38 58`	`21`	`Potential.`
`TRANSMEM`	`78 98`	`21`	`Potential.`
`REGION`	`99 212`	`114`	`Linker (By similarity).`
`REGION`	`213 628`	`416`	`Catalytic (By similarity).`
`ACT_SITE`	`307 307`		`Charge relay system (By similarity).`
`ACT_SITE`	`439 439`		`Charge relay system (By similarity).`
`ACT_SITE`	`515 515`		`Charge relay system (By similarity).`
`ACT_SITE`	`613 613`		`Proton donor (By similarity).`
`CARBOHYD`	`153 153`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`371 371`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`484 484`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`617 617`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`625 625`		`N-linked (GlcNAc...) (Potential).`

Sequence information

Length: 628 AA [This is the length of the unprocessed precursor]

Molecular weight: 67603 Da [This is the MW of the unprocessed precursor]

CRC64: BF7704C30EA334F5 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEASRRSAVK PVIVLKPSKR FPLVEDTPGK ASDALPLPLY LTNAVFFTLF FTVVYFLLSR 

        70         80         90        100        110        120 
WREKIRASIP LHAVTFPEIV AIFAFVASLI YLLGFFGIDF VQSLIIRPSG DVWSGEDYEE 

       130        140        150        160        170        180 
ENEVLLHEED ARTVPCGQAL DCSVPSLPHM ARNVTAQRLF DEKPVRVATE EDARKVSCGQ 

       190        200        210        220        230        240 
AVDCSLHSLP PRPPIVTSQK LFHEKTVIVT TEEDEEIIKS VVAGTLPSYS LESKLGDCKR 

       250        260        270        280        290        300 
AAAIRREALQ RLTGRSLSGL PLDGFDYESI LGQCCEMPVG YVQIPVGIAG PLLLNGREYS 

       310        320        330        340        350        360 
VPMATTEGCL VASTNRGCKA IHLSGGATSI LLKDGMTRAP VVRFSTAKRA AELKFYLEDP 

       370        380        390        400        410        420 
ENFDTLAVVF NRSSRFGRLQ SIKCAIAGKN LYLRFTCSTG DAMGMNMVSK GVQNVLDFLQ 

       430        440        450        460        470        480 
TDFPDMDVIG ISGNFCSDKK PAAVNWIEGR GKSVVCEAII EGDVVRKVLK TSVESLVELN 

       490        500        510        520        530        540 
MLKNLTGSAM AGALGGFNAH ASNIVTAIYI ATGQDPAQNV ESSHCITMME AVNDGKDLHI 

       550        560        570        580        590        600 
SVTMPSIEVG TVGGGTQLAS QSACLNLLGV KGASKDVAGA NSRMLATIVT GAVLAGELSL 

       610        620 
MSALAAGQLV KSHMKYNRSS KDMSNLSS

O64967 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools