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UniProtKB/Swiss-Prot entry O64900

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name C80B2_ESCCA
Primary accession number O64900
Secondary accession numbers None
Integrated into Swiss-Prot on December 15, 1998
Sequence was last modified on August 1, 1998 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 53)
Name and origin of the protein
Protein name (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
Synonyms EC 1.14.13.71
Cytochrome P450 80B2
Gene name
Name: CYP80B2
From
Eschscholzia californica (California poppy) [TaxID: 3467] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; Ranunculales; Papaveraceae; Eschscholzioideae; Eschscholzia.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
PubMed=9681018 [NCBI, ExPASy, EBI, Israel, Japan]
Pauli H.H., Kutchan T.M.;
"Molecular cloning and functional heterologous expression of two alleles encoding (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1), a new methyl jasmonate-inducible cytochrome P-450-dependent mono-oxygenase of benzylisoquinoline alkaloid biosynthesis.";
Plant J. 13:793-801(1998).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF014801; AAC39453.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T07963; T07963.
3D structure databases
HSSP P00179; 1DT6. [HSSP ENTRY / PDB]
ModBase O64900.
Ontologies
GO
GO:0005789; Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005792; Cellular component: microsome (inferred from electronic annotation from UniProtKB-KW).
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0020037; Molecular function: heme binding (inferred from electronic annotation from InterPro).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0050593; Molecular function: N-methylcoclaurine 3'-monooxygenase activity (inferred from electronic annotation from EC).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.
Gene3D G3DSA:1.10.630.10; Cyt_P450; 1.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PRINTS PR00463; EP450I.
PR00385; P450.
PROSITE PS00086; CYTOCHROME_P450; 1.
Other
ProtoNet O64900.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   488  488     (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2. PRO_0000052158
TRANSMEM   3    23  21     Potential. 
METAL   430   430        Iron (heme axial ligand) (By similarity). 
Sequence information
Length: 488 AA [This is the length of the unprocessed precursor] Molecular weight: 54896 Da [This is the MW of the unprocessed precursor] CRC64: 5DBFFECBAAC1A3F2 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MEVVTVALIA VIISSILYLL FGSSGHKNLP PGPKPWPIVG NLLQLGEKPH AQFAELAQTY 

        70         80         90        100        110        120 
GDIFTLKMGT ETVVVASTSS AASEILKTHD RILSARYVFQ SFRVKGHVEN SIVWSDCTET 

       130        140        150        160        170        180 
WKNLRKVCRT ELFTQKMIES QAHVREKKCE EMVEYLMKKQ GEEVKIVEVI FGTLVNIFGN 

       190        200        210        220        230        240 
LIFSQNIFEL GXPNSGSSEF KEYLWRMLEL GNSTNPADYF PMLGKFDLFG QRKEVAECLK 

       250        260        270        280        290        300 
GIYAIWGAML QERKLAKKVD GYQSKNDFVD VCLDSGLNDY QINALLMELF GAGTETSAST 

       310        320        330        340        350        360 
IEWAMTELTK NPKITAKLRS ELQTVVGERS VKESDFPNLP YLEATVKETL RLHPPTPLLL 

       370        380        390        400        410        420 
PRRALETCTI LNYTIPKDCQ IMVNAWGIGR DPKTWIDPLT FSPERFLNSS VDFRGNDFSL 

       430        440        450        460        470        480 
IPFGAGRRIC PGLPIANQFI ALLVATFVQN LDWCLPNGMS VDHLIVEEKF GLTLQKEPPL 


FIVPKSRV
O64900 in FASTA format

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