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UniProtKB/Swiss-Prot entry O55528

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name L_SENDO
Primary accession number O55528
Secondary accession number O55530
Integrated into Swiss-Prot on February 1, 2005
Sequence was last modified on June 1, 1998 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 47)
Name and origin of the protein
Protein name Large structural protein
Synonyms Protein L
Transcriptase
Replicase
Includes RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)
Gene name
Name: L
From
Sendai virus (strain Ohita) (SeV) [TaxID: 302272] 
Taxonomy Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Respirovirus.
Virus hosts Cavia cutleri (Guinea pig) [TaxID: 10144]
Cricetidae sp. (Hamster) [TaxID: 36483]
Mus musculus (Mouse) [TaxID: 10090]
Rattus norvegicus (Rat) [TaxID: 10116]
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
STRAIN=Isolate M1, and Isolate MVC11;
PubMed=9400971 [NCBI, ExPASy, EBI, Israel, Japan]
Itoh M., Isegawa Y., Hotta H., Homma M.;
"Isolation of an avirulent mutant of Sendai virus with two amino acid mutations from a highly virulent field strain through adaptation to LLC-MK2 cells.";
J. Gen. Virol. 78:3207-3215(1997).
Comments
  • FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently six subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals. 5' GpppApGpG sequence is required for mRNA cap methylation by the enzyme (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
  • SUBUNIT: Homooligomer. Interacts with the P and C proteins. The L protein complexes with P protein to form the functional polymerase. C protein binding to L has an inhibitory effect (By similarity).
  • SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
  • DOMAIN: The N-terminal part (about 1-400) seems to be involved in binding to the P protein (By similarity).
  • MISCELLANEOUS: Least abundant structural protein (approximately 50 copies per virion). Unstable in the absence of P protein (By similarity).
  • SIMILARITY: Belongs to the paramyxoviruses L protein family.
  • SIMILARITY: Contains 1 RdRp catalytic domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB005795; BAA24392.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB005796; BAA24401.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_056879.1; -.
3D structure databases
ModBase O55528.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0019012; Cellular component: virion (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003968; Molecular function: RNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0006370; Biological process: mRNA capping (inferred from electronic annotation from UniProtKB-KW).
GO:0006410; Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.
Pfam PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000830; RNA_pol_ParamyxoV; 1.
PROSITE PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
GeneID 1489777; -.
Other
ProtoNet O55528.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   2228  2228     Large structural protein. PRO_0000142739
DOMAIN   656    840  185     RdRp catalytic. 
NP_BIND   1801   1810  10     ATP (Potential). 
REGION   1    174  174     Oligomerization domain (By similarity). 
REGION   1756   2228  473     Involved in mRNA cap methylation (By similarity). 
COMPBIAS   2031   2034  4     Poly-Ser. 
VARIANT   2050   2050  1     E -> A (in avirulent isolate MVC11). 
Sequence information
Length: 2228 AA [This is the length of the unprocessed precursor] Molecular weight: 253061 Da [This is the MW of the unprocessed precursor] CRC64: 9BDAC6F266B13B1F [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MDGQESTQNP SDILYPECHL NSPIVRGKIA QLHVLLDVNQ PYILKDDSII NITKHKIRNG 

        70         80         90        100        110        120 
GLSLRQIKIR SLGKALQRTI KDLDRYTFEP YPTYSQELLR LDIPEICDKI RSVFAVSDRL 

       130        140        150        160        170        180 
TKELSNGFQD LWLNIFKQLG NIEGREGYDP LQDISTIPEI TERYSRNKWY RPFLTWFSIK 

       190        200        210        220        230        240 
YDMRWMQKTR PGGPLDTSNS HNLLECKSYT LVTYGDLVMI LNKSTLTGYI LTPELVLMYC 

       250        260        270        280        290        300 
DVVEGRWNMS AAGQLDKRST GITSKGEELW ELVDSLFSSL GEEIYNVIAL LEPLSLALIQ 

       310        320        330        340        350        360 
LSDPVIPLRG AFMRHVLTEL QTVLTSKDVY TDPEADAIVE SLLAIFHGTS IDEKAEIFSF 

       370        380        390        400        410        420 
FRTFGHPSLE AVTAADKVRA HMYAQKAIKL KTLHECHAVF CTIIINGYRE RHGGQWPPCD 

       430        440        450        460        470        480 
FPDHVCLELR NAQGSNTAIS YECAVDNYTS FIGFKFRKFI EPQLDEDLTI YMKDKALSPR 

       490        500        510        520        530        540 
KEAWDSVYPD SNLYYKVPES EETRRLIEVF INDENFNPED IIDYVESGDW LKDEKFNISY 

       550        560        570        580        590        600 
SLKEKEIKQE GRLFAKMTYK MRAVQVLAET LLAKGIGELF SENGMVKGEI DLLKRLTTLS 

       610        620        630        640        650        660 
VSGVPRTDSV YNNPRSSEKR NESMKKRNSK GYWDEKKRSR HEFKATDSST DGYETLSCFL 

       670        680        690        700        710        720 
TTDLKKYCLN WRFESTALFG QRCNEIFGFK TFFNWMHPVL EKCTIYVGDP YCPVADRMHR 

       730        740        750        760        770        780 
QLQDHADSGI FIHNPRGGIE GYCQKLWTLI SISAIHLAAV RVGVRVSAMV QGDNQAIAVT 

       790        800        810        820        830        840 
SRVPVAQTYK QKKNHVYEEI TRYFGALRHV MFDIGHELKL NETIISSKMF VYSKRIYYDG 

       850        860        870        880        890        900 
KILPQCLKAL TRCVFWSETL VDENRSACSN ISTSIAKAIE NGYSPILGYC IALYKTCQQV 

       910        920        930        940        950        960 
CISLGMTINP TISPTVRDQY FKGKNWLRCA VLIPANVGGF NYMSTSRCFV RNIGDPAVAA 

       970        980        990       1000       1010       1020 
LADLKRFIRA DLLDKQVLYR VMNQEPGDSS FLDWASDPYS CNLPHSQSIT TIIKNITARS 

      1030       1040       1050       1060       1070       1080 
VLQESPNPLL SGLFTETSGE EDLNLASFLM DRKVILPRVA HEILSNSLTG VREAIAGMLD 

      1090       1100       1110       1120       1130       1140 
TTKSLVRASV KRGGLSYGIL RRLVNYDLLQ YETLTRTLRK PVKDNIEYEY MCSVELAVGL 

      1150       1160       1170       1180       1190       1200 
RQKMWIHLTY GRPIHGLETP DPLELLRGTF IEGSEVCKLC RSEGADPIYT WFYLPDNIDL 

      1210       1220       1230       1240       1250       1260 
DTLTNGSPAI RIPYFGSATD ERSEAQLGYV RNLSKPAKAA IRIAMVYTWA YGTDEISWME 

      1270       1280       1290       1300       1310       1320 
AALIAQTRAN LSLENLKLLT PVSTSTNLSH RLKDTATQMK FSSATLVRAS RFITISNDNM 

      1330       1340       1350       1360       1370       1380 
ALKEAGESKD TNLVYQQIML TGLSLFEFNM RYKKGSLEKP LILHLHLNNG CCIMESPQEA 

      1390       1400       1410       1420       1430       1440 
NIPPRSTLDL EITQENNKLI YDPDPLRDVD LELFSKVRDV VHTVDMTYWS DDEVIRATSI 

      1450       1460       1470       1480       1490       1500 
CTAMTIADTM SQLDRDNLKE MIALVNDDDV NSLITEFMVI DVPLFCSTFG GILVNQFAYS 

      1510       1520       1530       1540       1550       1560 
LYGLNIRGRE EIWGHVVRIL KDTSHAVLKV LSNALSHPKI FKRFWNAGVV EPVYGPNLSN 

      1570       1580       1590       1600       1610       1620 
QDKTLLALSV CEYSVDLFMH DWQGGVPLEV FICDNDPDVA DMRRSSFLAR HLAYLCSLAE 

      1630       1640       1650       1660       1670       1680 
ISRDGPRLES MNSLERLETL KSYLELTFLD DPVLRYSQLT GLVIKVFPST LTYIRKSSIK 

      1690       1700       1710       1720       1730       1740 
VLRTRGIGVP EVLEDWDPEA DNALLDGIAA EIQQNIPLGH QTRAPFWGLR VSKSQVLRLR 

      1750       1760       1770       1780       1790       1800 
GYEEITRGEV GRSGVGLTLP FDGRYLSHQL RLFGVNSTSC LKALELTYLL SPLVDKDKDR 

      1810       1820       1830       1840       1850       1860 
LFLGEGAGAM LSCYDATLGP CINYYNSGVY SCDVNGQREL NIYPAEVALV GKKLNNVTSL 

      1870       1880       1890       1900       1910       1920 
GQRVKVLFNG NPGSTWIGND ECEALIWNEL QNNSIGLVHC DMEGGDHKDD QVVLHEHYSV 

      1930       1940       1950       1960       1970       1980 
IRIAYLVGDR DVVLISKIAP RLGTDWTRQL SLYLRYWDEV NLVVLKTSNP ASTEMYLLSR 

      1990       2000       2010       2020       2030       2040 
HPKSDIIEDS KTVLASLHPL SKEDSIKIEK WILIEKAKAH EWVTRELREG SSSSGMLRPY 

      2050       2060       2070       2080       2090       2100 
HQALQTFGFE PNLYKLSRDF LSTMNIADTH NCMTAFNRVL KDTIFEWARI TESDKRLKLT 

      2110       2120       2130       2140       2150       2160 
GKYDLYPVRD SGKLKTISRR LVLSWVSLSM STRLVTGSFP DQKFEARLQL GIVSLSSREI 

      2170       2180       2190       2200       2210       2220 
RNLRVITKTI LDRFENTIHS ITYRFLTKEV KILMKILGAV KMFGARQNEY TTVVDDGSLD 


DIEPYDSL
O55528 in FASTA format

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