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[1]
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NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, INDUCTION, AND TISSUE SPECIFICITY.
TISSUE=Prostate;
DOI=10.1074/jbc.272.25.15959; PubMed=9188497 [NCBI, ExPASy, EBI, Israel, Japan]
Biswas M.G.,
Russell D.W.;
"Expression cloning and characterization of oxidative 17beta- and 3alpha-hydroxysteroid dehydrogenases from rat and human prostate.";
J. Biol. Chem. 272:15959-15966(1997).
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[2]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Liver;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
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- FUNCTION: NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro) (By similarity). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro).
- CATALYTIC ACTIVITY: Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H.
- CATALYTIC ACTIVITY: Testosterone + NAD+ = androst-4-ene-3,17-dione + NADH.
- CATALYTIC ACTIVITY: Retinol + NAD+ = retinal + NADH.
- ENZYME REGULATION: Competitively inhibited by 9-cis-retinoic ancid and 13-cis-retinoic acid.
- BIOPHYSICOCHEMICAL PROPERTIES:
| Kinetic parameters: |
KM=0.1 µM for 5-alpha-androstan-3-alpha,17-beta-diol; | | KM=0.2 µM for androsterone; | | KM=0.5 µM for dihydrotestosterone; | | KM=1.1 µM for testosterone; | | KM=0.8 µM for estradiol; | | KM=3 µM for NAD; | | KM=1 mM for NADP; | | Vmax=2.5 nmol/min/mg enzyme for 5-alpha-androstan-3-alpha,17- beta-diol; | | Vmax=0.1 nmol/min/mg enzyme for androsterone; | | Vmax=0.5 nmol/min/mg enzyme for dihydrotestosterone; | | Vmax=1.1 nmol/min/mg enzyme for testosterone; | | Vmax=2.1 nmol/min/mg enzyme for estradiol; | | Note=Vmax was measured using transfected cell lysates; | |
- SUBCELLULAR LOCATION: Microsome membrane; Peripheral membrane protein; Lumenal side (By similarity). Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side (By similarity).
- TISSUE SPECIFICITY: Detected in prostate, liver and kidney.
- INDUCTION: Up-regulated by testosterone. Levels are very low in castrated male rats.
- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 317 AA [This is the length of the unprocessed precursor] |
Molecular weight: 36145 Da [This is the MW of the unprocessed precursor] |
CRC64: 4AB3F3160C6675A9 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MWFYLVTLVG LYYLLRWYRE RQVVSHLHDK YVFITGCDSG FGNLLARQLD RRGMRVLAAC
70 80 90 100 110 120
LTEKGAEELK SKTSDRLETV ILDVTNTDSI SAATQWVKEH VGDKGLWGLV NNAGVFQAFA
130 140 150 160 170 180
YIEWCRPEDC MSIFQVNLIG LAQVTLSMLF LVKKARGRIV NVSSVLGRVA LFGGFYSCSK
190 200 210 220 230 240
YGVEAFSDVL RREIRDFGVK VSIIEPGSFK TRMTDAELII EKTKKTWEAT PEHIRESYGQ
250 260 270 280 290 300
QFFDDFCNTT RRELKKCSTN LSLVTDCMEH ALTSKYPRTR YSAGWDARLF FIPLSYLPTS
310
LVDCLLAISR RKPAQAV
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O54753 in FASTA format |
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