NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=66 / 1326;
PubMed=9515933 [NCBI, ExPASy, EBI, Israel, Japan]
Blanco J., Coque J.R., Martin J.;
"The folate branch of the methionine biosynthesis pathway in Streptomyces lividans: disruption of the 5,10-methylenetetrahydrofolate reductase gene leads to methionine auxotrophy.";
J. Bacteriol. 180:1586-1591(1998).

Comments

CATALYTIC ACTIVITY: 5-methyltetrahydrofolate + NAD(P)⁺ = 5,10-methylenetetrahydrofolate + NAD(P)H.
COFACTOR: FAD (By similarity).
PATHWAY: One-carbon metabolism; tetrahydrofolate pathway .
SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ001630; CAA04885.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P00394; 1B5T. [HSSP ENTRY / PDB]

ModBase

O54235.

Ontologies

GO:0004489;	Molecular function: methylenetetrahydrofolate reductase (NADPH) activity (inferred from electronic annotation from InterPro).
GO:0009086;	Biological process: methionine biosynthetic process (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR003171; Mehydrof_redctse.
IPR004620; MTHF_reductase_bac.
Graphical view of domain structure.

Pfam

PF02219; MTHFR; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00676; fadh2; 1.

Other

ProtoNet

O54235.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Amino-acid biosynthesis; FAD; Flavoprotein; Methionine biosynthesis; NAD; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 307`	`307`		`5,10-methylenetetrahydrofolate reductase.`	`PRO_0000190266`

Sequence information

Length: 307 AA [This is the length of the unprocessed precursor]

Molecular weight: 33268 Da [This is the MW of the unprocessed precursor]

CRC64: 0CA09C336036D8A9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MALGTASTRT DRARTVRDIL ATGKTTYSFE FSAPKTPKGE KNLWSALRRV EAVAPDFVSV 

        70         80         90        100        110        120 
TYGAGGSTRA GTVRETQQIV ADTTLTPVAH LTAVDHSVAE LRNIIGQYAD AGIRNMLAVR 

       130        140        150        160        170        180 
GDPPGDPNAD WIAHPEGLTY AAELVRLIKE SGDFCVGVAA FPEMHPRSAD WDTDVTNFVD 

       190        200        210        220        230        240 
KCRAGADYAI TQMFFQPDSY LRLRDRVAAA GCATPVIPEV MPVTSVKMLE RLPKLSNASF 

       250        260        270        280        290        300 
PAELKERILT AKDDPAAVRS IGIEFATEFC ARLLAEGVPG LHFITLNNST ATLEIYENLG 


LHHPPRA

O54235 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools