FUNCTION: Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions (By similarity).
CATALYTIC ACTIVITY: Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin.
COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer (By similarity).
SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Single-pass membrane protein (By similarity). Note=The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer (By similarity).
MISCELLANEOUS: This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.
MISCELLANEOUS: The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.
SIMILARITY: Contains 1 Rieske domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF037456; AAC04807.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P08980; 1RFS. [HSSP ENTRY / PDB]

SMR

O49078; 105-230.

ModBase

O49078.

Ontologies

GO:0009507;	Cellular component: chloroplast (inferred from electronic annotation from UniProtKB-KW).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0042651;	Cellular component: thylakoid membrane (inferred from electronic annotation from InterPro).
GO:0051537;	Molecular function: 2 iron, 2 sulfur cluster binding (inferred from electronic annotation from InterPro).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009496;	Molecular function: plastoquinol-plastocyanin reductase activity (inferred from electronic annotation from InterPro).
GO:0022900;	Biological process: electron transport chain (inferred from electronic annotation from UniProtKB-KW).
GO:0006810;	Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR014909; Cyt_b6-F_Fe-S_su.
IPR014349; Rieske.
IPR005805; Rieske_C.
IPR005806; Rieske_reg.
Graphical view of domain structure.

Gene3D

G3DSA:2.102.10.10; Rieske_reg; 1.

PANTHER

PTHR10134; Rieske; 1.

Pfam

PF08802; CytB6-F_Fe-S; 1.
PF00355; Rieske; 1.
Pfam graphical view of domain structure.

PRINTS

PR00162; RIESKE.

PROSITE

PS51296; RIESKE; 1.
PROSITE graphical view of domain structure (profiles).

Other

ProtoNet

O49078.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; Chloroplast; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Oxidoreductase; Plastid; Thylakoid; Transit peptide; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 56`	`56`	`Chloroplast (Potential).`
`CHAIN`	`57 230`	`174`	`Cytochrome b6-f complex iron-sulfur subunit, chloroplastic.`	`PRO_0000030689`
`TRANSMEM`	`73 93`	`21`	`Potential.`
`DOMAIN`	`116 212`	`97`	`Rieske.`
`METAL`	`158 158`		`Iron-sulfur (2Fe-2S) (By similarity).`
`METAL`	`160 160`		`Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity).`
`METAL`	`176 176`		`Iron-sulfur (2Fe-2S) (By similarity).`
`METAL`	`179 179`		`Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity).`
`DISULFID`	`163 178`		`By similarity.`

Sequence information

Length: 230 AA [This is the length of the unprocessed precursor]

Molecular weight: 24298 Da [This is the MW of the unprocessed precursor]

CRC64: D1B914F0049AF51E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MASTTLSATP TPSQLSAAKN GAYSPSRALL GKTARGLYPE KEMVSRKVTC QATSIPADRV 

        70         80         90        100        110        120 
PDMGKRQTMN LLLLGALSLP TAGMLIPYGA FFVPPSSGGG GGGIVAKDAV GNDIVAAAWL 

       130        140        150        160        170        180 
KTHGPGDRTL AQGLRGDPTY LVVENDRSLA TYGINAVCTH LGCVVPWNKA ENKFLCPCHG 

       190        200        210        220        230 
SQYNNQGKVV RGPAPLSLAL SHCDISEEGK VVFVPWVETD FRTGENPWWS

O49078 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools